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Plasmid#168462PurposeBig-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA-P2A-mScarlet expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.DepositorTypeEmpty backboneUseCre/Lox, Mouse Targeting, and Synthetic Biology ;…TagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only
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pLP-PIGA3 (pLP305)
Plasmid#196992PurposeBig-IN Landing Pad plasmid encoding pEF1a-driven PuroR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites, sleeping beauty ITRs and BsmBI homology arms cloning sites.DepositorTypeEmpty backboneUseCre/Lox, Mouse Targeting, and Synthetic Biology ;…TagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP
Plasmid#50834PurposeAn AAV packaging vector that expresses channel rhodopsin 2 (H134R) (fused to EYFP) under the EF1a promoter, which can be deactivated (deleted) by recombination between the flanking loxP sites.DepositorInserthChR2 (H134R)
UseAAV and Cre/LoxTagsEYFPExpressionMammalianMutationH134RPromoterEF1aAvailable sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGECPL.BC10.MG
Plasmid#223822PurposeContains Cas9-editable barcode, marking guide (MG) for lineage tracing, Cre for Cas9 activation and loxP-mediated gene deletion, and Firefly Luciferase (FLuc) for live luminescence-based monitoring.DepositorInsertEf1a-Driven Cre-P2A-FLuc
UseCRISPR, Cre/Lox, Lentiviral, Luciferase, and Mous…TagsP2AExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pW34
Plasmid#158210PurposeLuxCDABE operon (R6K ori) under T7, in loxP and lox5171 sites.DepositorInsertLuxABCDE
UseTagsExpressionMutationPromoterAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pW5Y_Apr
Plasmid#158211PurposeBAC-based, lacUV5 regulon and Cre recombinase in loxP and lox5171 sitesDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
p53 S23A targeting vector
Plasmid#12167DepositorInsertp53 (Trp53 Mouse)
UseCre/LoxTagsExpressionMammalianMutationSer23 mutation (S23A) in exon 2 and puromycin gen…PromoterAvailable sinceJune 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
AA365
Plasmid#215969PurposeFragmid fragment: (Pol 2 promoter) Gal4-induced expressionDepositorHas ServiceCloning Grade DNAInsertLoxP_v1; 5xUAS_v1; LoxP_v1; 5xUAS_v1; Hsp70_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pW17
Plasmid#158207Purpose2lox landing pad plasmid with mariner transposon and transposase used for CRAGEDepositorInsertloxP/Cre/KmR/Lox5171/Mariner IR oriT/transposase
UseTagsExpressionMutationPromoterAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
dRMCE Plasmids
Plasmid Kit#1000000015PurposeTakes advantage of wild-type loxP and FRT sites present in these conditional alleles in mouse embryonic stem cells to introduce tags, reporters, and mutant coding regions into an endogenous lociDepositorAvailable sinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pT3EF1aH-myr-Akt
Plasmid#179909PurposeThis plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used with Cre for in vivo studies.DepositorInsertAkt1 (Akt1 Mouse)
UseTagsHA and MyrExpressionMammalianMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pREPROII-attB
Plasmid#86557PurposeDox inducible expression of reprogramming factors (Klf4, Sox2, Oct4, & cMyc), integration via phiC31 attB sites, excision via Cre/loxPDepositorUseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMyt1GFPicre-Knock-In
Plasmid#22657DepositorInsertMyelin Transcription Factor 1 (Myt1 Mouse)
UseTargeting vectorTagsExpressionMutationPromoterAvailable sinceNov. 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUltra-puro-RTTA3
Plasmid#58750Purposesilence doxycyclin inducible pUltra-doxDepositorInsertReverse TET-Transactivator 3
UseLentiviralTagsExpressionMammalianMutationloxP5171PromoterUbiquitin C promoterAvailable sinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUltra-Smurf-luc-RTTA3
Plasmid#59438Purposesilence doxycyclin inducible pUltra-dox (also expresses luciferase)DepositorInsertsluciferase
Reverse TET-Transactivator 3
UseLentiviralTagsExpressionMammalianMutationloxP5171PromoterAvailable sinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMVS223
Plasmid#176293PurposeSyntheticTranscriptionFactor_loxFAS_PGK_MCS_scarlett_ERD_ZIFDBD_P2A_Neo_loxP_AMPDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pMLS253
Plasmid#114271PurposeSapTrap donor vector for point mutagenesis; pENTR-221::syntron(loxp:unr-119+)DepositorInsertCbr-Unc-119
UseCRISPR and Cre/LoxTagsExpressionMutation*please see depositor comment belowPromoterAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Sox17 Flox
Plasmid#23335DepositorInsertSex determining region Y-box containing gene 17 (Sox17 Mouse)
UseMouse TargetingTagsExpressionMutationNonePromoterAvailable sinceJuly 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pC-(lox2-attB2-SA-T2A-Gal4-Hsp70)3
Plasmid#62957PurposeTriplet donor for in vivo RMCE with MiMIC inserts to make Gal4 driver.DepositorInsertT2A-Gal4 in three phases
UseTagsExpressionMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only