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Plasmid#110086PurposeSuicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.DepositorTypeEmpty backboneUseBacterial knockout generationTagsExpressionMutationPromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only
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pSTARR-seq_fly
Plasmid#71499PurposeflySTARR-seq screening vector with DSCP core promoter.DepositorTypeEmpty backboneUseFlystarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionInsectMutationPromoterAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKO (Apr)
Plasmid#110088PurposeSuicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.DepositorTypeEmpty backboneUseBacterial knockout generationTagsExpressionMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKO (Hyg)
Plasmid#110087PurposeSuicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.DepositorTypeEmpty backboneUseBacterial knockout generationTagsExpressionMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT7-L1T1L-NLuc
Plasmid#128942PurposeExpresses L1-NLuc geneDepositorInsertL1 minor core protein
UseTagsNanolucExpressionBacterialMutationPromoterT7 promotorAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMIAII-Cd4
Plasmid#194401PurposeRetroviral expression of mouse CD4DepositorInsertCd4 (Cd4 Mouse)
UseRetroviralTagsExpressionMutationWTPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet_WTDHFR_WTTS
Plasmid#91226Purposebacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genesDepositorInsertsfolA
thyA
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM733
Plasmid#68898PurposeIPTG-inducible CRISPRi vector targeting NanoLuc (NL4), constiutive NanoLuc, pNBU2 backbone, AmpRDepositorInsertsdCas9
LacI
sgRNA
NanoLuc
UseTagsExpressionMutationPromoterAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-PCYT2
Plasmid#81074PurposeExpresses PCYT2 in mammalian cellsDepositorInsertphosphate cytidylyltransferase 2, ethanolamine (PCYT2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSTARR-seq_fly-hsp70
Plasmid#71500PurposeflySTARR-seq screening vector with hsp70 core promoterDepositorTypeEmpty backboneUseFlystarr-seq screening vectorTagssgGFP (SuperGlo, Qbiogene, Inc)ExpressionInsectMutationPromoterAvailable sinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMINTNH3
Plasmid#110074PurposeIntegrative vector for expression in mycobacteria based on the Tet promoter. Contains an N-terminal His10 Tag followed by a 3C cleavage siteDepositorTypeEmpty backboneUseTagsHis10 Tag - 3C cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMINTC3GH (Hyg)
Plasmid#110077PurposeAnalogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMEXC3GH (Hyg)
Plasmid#110082PurposeAnalogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMEXC3GH (Kan)
Plasmid#110081PurposeAnalogous to pMINTC3GH but containing oriM for episomal replication in mycobacteria.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMINTC3GH (Kan)
Plasmid#110076PurposeAnalogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMM731
Plasmid#68896PurposeIPTG-inducible CRISPRi vector targeting NanoLuc (NL1), constiutive NanoLuc, pNBU2 backbone, AmpRDepositorInsertsdCas9
LacI
sgRNA
NanoLuc
UseTagsExpressionMutationPromoterAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
KG#45
Plasmid#110926PurposeVector for expressing proteins in most or all tissues, including nervous system, under control of the inducible heat shock promoter in C. elegansDepositorInsertshsp-16-2 promoter
unc-54 3' control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMVTCRa_candidate5
Plasmid#164999PurposeExpression of candidate TCR #5 TCRalpha cDNADepositorInsertTRAV (TRA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMVTCRb_candidate5
Plasmid#165000PurposeExpression of candidate TCR #5 TCRbeta cDNADepositorInsertTRB (TRB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCoofy41
Plasmid#55184PurposeInsect expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneUseTagsHis10-Stop-OneStrep-Stop-S-tag-Stop-CBP-HPC4-Stop…ExpressionBacterialMutationPromoterpPH (Polyhedrin promoter)Available sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only