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Plasmid#164784PurposeNanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptorsDepositorInsertNanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Oplophorus gracilirostris, Human)
UseTagsNanoLucExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_PAX5_NOL4L
Plasmid#205886PurposeExpress mEGFP-tagged fusion protein, PAX5_NOL4L from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLigify_Isoeugenol
Plasmid#215563PurposeExpresses GFP in response to IsoeugenolDepositorInsertsGFP mut2
iemR
UseTagsExpressionBacterialMutationPromoterP250 and PiemrAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLigify_4Vinylphenol
Plasmid#215564PurposeExpresses GFP in response to 4-VinylphenolDepositorInsertsGFP mut2
vprR
UseTagsExpressionBacterialMutationPromoterPvprrAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUAS-NanoLuc
Plasmid#87696PurposeGal4VP16 driven expression of NanoLucDepositorInsertNanoLuc
UseTagsnuclear localization signalExpressionMammalianMutationPromoterAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-NanoLuc
Plasmid#189975PurposePart 3-4-5 plasmid to introduce 3xFLAG-NanoLuc into circRNAsDepositorInsertNanoLuc
UseCloningTags3xFLAGExpressionMutationPromoterN/AAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB-TopFlash-NanoLucP
Plasmid#204347PurposeTopFlash reporterDepositorInsertTopFlash-NanoLucP
UseLuciferaseTagsNanoLucPExpressionMutationPromoterminiPAvailable sinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
NanoLuc-PM-Venus
Plasmid#164786PurposeNanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent proteinDepositorInsertNanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Oplophorus gracilirostris, Human)
UseTagsNanoLuc and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNH-NanoLuc
Plasmid#173075PurposeExpression vector for E. coli producing target protein with N-terminal Nanoluciferase fusionDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV_CMV_CCR6-Nanoluc-V5
Plasmid#199672Purposeexpresses CCR6-NanoLuc-V5 in mammalian cells, AAV vectorDepositorInsertCCR6 (CCR6 Human)
UseAAVTagsNanoLuc and V5ExpressionMammalianMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LiPOP1-NanoLuc
Plasmid#168265PurposeChemo-optogenetic control of necroptotic cell death by coupling LiPOP1 with NanoLuc-aided bioluminescence optogenetic stimulation (NanoLOGS)DepositorInsertMLKL(1-125) (MLKL Human)
UseTagsmCherry, NanoLuc, CRY2-PHRExpressionMammalianMutationH15A, K16A, R17APromoterCMVAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-NanoLuc
Plasmid#67178PurposeCRISPaint universal donor for NanoLuc fusion taggingDepositorInsertNanoLuc
UseTagsExpressionMutationPromoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR_221_middle-entry_NanoLuc
Plasmid#128673PurposeNanoLuc CDS middle entry gateway cloneDepositorInsertNanoLuc
UseTagsExpressionMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
NanoLuc-SZ2
Plasmid#176645PurposeNanoLuc enzyme linked with SynZip2DepositorInsertNanoLuc-SZ2
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA2-LA-nanoLuc-RA
Plasmid#128692PurposeDonor plasmid that promotes integration of the NanoLuc coding sequence at the apobb.1 locusDepositorInsertapobb.1 NanoLuc
UseMultisite-gateway assemblyTagsNanoLucExpressionMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ydf NanoLuc
Plasmid#225656PurposeReporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.DepositorInsertNanoLuciferase
UseCell free expressionTagsMycExpressionBacterialMutationPromoterPydfJ115Available sinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57_NRAS-5'UTR_Nanoluc
Plasmid#219990PurposeNRAS-5'UTR-NanoLuc mRNA synthesisDepositorInsertNRAS-5'UTR-NanoLuc (NRAS Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-Quartet_NoLinker
Plasmid#214731PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 with no linkers between the RBDs and an N-terminal SpyTagDepositorInsertSpyTag-Quartet_NoLinker (S Synthetic)
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBP-C_NanoLuc
Plasmid#204112PurposeType 0 CDS partDepositorInsertNanoLuc luciferase
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
RPL13A_PQR_mScarlet_NOL_LoxP_PQR_zeo_PQR_MCS_RPL13A-3'UTR
Plasmid#165043PurposeRepair template for CAS9 complex genome editingDepositorInsertRPL13A (RPL13A Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only