Addgene

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T-B18

Bacterial Strain

#159446

Purpose

E. coli BW25113 ΔfrmA, harboring plasmid Addgene plasmid # 129104, was adaptively evolved to grow efficiently on threonine and perform significant biosynthesis while employing methanol-derived carbon

Depositor

Maciek R. Antoniewicz, Eleftherios Terry Papoutsakis

Article

Har et al Appl Microbiol Biotechnol. 2021 Jan;105(

Bacterial Resistance

Ampicillin and kanamycin

Available since

Aug. 18, 2021

Availability

Industry, Academic Institutions, and Nonprofits
US0hisB-pyrF-

Bacterial Strain

#12614

Depositor

Scot Wolfe

Article

Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

Bacterial Resistance

Tetracycline

Available since

Sept. 26, 2006

Availability

Academic Institutions and Nonprofits only
C321.∆A T7RNAP

Bacterial Strain

#182776

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Spectinomycin

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
MW005 (bacterial strain)

Bacterial Strain

#24545

Depositor

Colin Dolphin

Article

Westenberg et al BMC Biotechnol. 2010 Mar 29. 10(1):27.

Bacterial Resistance

Streptomycin

Available since

Sept. 13, 2010

Availability

Academic Institutions and Nonprofits only
DHIP1

Bacterial Strain

#226851

Purpose

Expresses HIP1 methyltransferase to methylate plasmids for delivery to cyanobacteria

Depositor

David Nielsen

Article

Kamoku et al Biotechnol Prog. 2025 Jan 23:e3518. doi:

Bacterial Resistance

Streptomycin

Species

Escherichia coli

Available since

Nov. 15, 2024

Availability

Academic Institutions and Nonprofits only
C321.∆A ompT- rne- endA-

Bacterial Strain

#182774

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA inactivated

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Ampicillin

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
C321.∆A ompT- rne- endA- lon-

Bacterial Strain

#182775

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, endA, lon inactivated

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Ampicillin

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
C321.∆A T7RNAP ompT- rne-

Bacterial Strain

#182777

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT and rne inactivated

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Spectinomycin

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
C321.∆A ompT- rne- lon-

Bacterial Strain

#182773

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); ompT, rne, lon inactivated

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Ampicillin

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
C321.∆A T7RNAP ompT- rne- lon-

Bacterial Strain

#182778

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted; ompT, rne, and lon inactivated

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Spectinomycin

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
TB204

Bacterial Strain

#230033

Purpose

Fluorescently labelled (monomeric sfGFP) E. coli

Depositor

Tobias Bergmiller, Calin Guet

Article

Tomasek et al J Biotechnol. 2018 Feb 20;268:40-52. doi

Bacterial Resistance

None

Available since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only
TB201

Bacterial Strain

#230031

Purpose

Fluorescently labelled (YFP) E. coli

Depositor

Tobias Bergmiller, Calin Guet

Article

Tomasek et al J Biotechnol. 2018 Feb 20;268:40-52. doi

Bacterial Resistance

None

Available since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only
RHO3

Bacterial Strain

#124700

Purpose

E. coli mobilizer strain that facilitates conjugation of mobilizable plasmids by using metabolic counter-selection against the donor strain.

Depositor

Herbert Schweizer

Article

Lopez et al Appl Environ Microbiol. 2009 Oct;75(20):

Bacterial Resistance

Dap (400 µg/ml)

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only
C321.deltaA (Isaacs lab)

Bacterial Strain

#73581

Purpose

Genomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB gene

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Bacterial Resistance

Ampicillin

Available since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
DH10B E. coli aptI/gidB::Landing Pad

Bacterial Strain

#83036

Purpose

For site-specific chromosomal insertion of a target DNA sequence at an engineered landing pad site in the atpI/gidB locus.

Depositor

Cammie Lesser

Article

Reeves et al ACS Synth Biol. 2015 May 15;4(5):644-54.

Bacterial Resistance

Tetracycline

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only
ML40K1

Bacterial Strain

#61920

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG argH metA lysA genes. deleted. Contains pACYC plasmid.

Depositor

Robert Gennis, Toshio Iwasaki

Article

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Bacterial Resistance

Chloramphenicol

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only

Showing: 1 - 16 of 16 results

Results per page:

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T-B18

US0hisB-pyrF-

C321.∆A T7RNAP

MW005 (bacterial strain)

DHIP1

C321.∆A ompT- rne- endA-

C321.∆A ompT- rne- endA- lon-

C321.∆A T7RNAP ompT- rne-

C321.∆A ompT- rne- lon-

C321.∆A T7RNAP ompT- rne- lon-

TB204

TB201

RHO3

C321.deltaA (Isaacs lab)

DH10B E. coli aptI/gidB::Landing Pad

ML40K1

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