Addgene

We narrowed to 14,048 results for: LEA

Showing: 541 - 560 of 14048 results

Results per page:

pcDNA3.1-Cerulean-Epac2(L426W)-Venus

Plasmid

#138200

Purpose

FRET-based Epac2 activation sensor with L426W mutation in Epac2 coding sequence.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2(L426W)-Venus (Rapgef4 Synthetic, Mouse)

Use

Tags

Cerulean and Venus

Expression

Mammalian

Mutation

Contains leucine-to-tryptophan substitution at re…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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PhOTO-N (pMTB:memb-Cerulean-2A-H2B-Dendra2)

Plasmid

#92400

Purpose

Constitutive expression of nuclear green-to-red photoconvertible Dendra2 and the membrane blue fluorescent protein Cerulean.

Depositor

Periklis Pantazis

Article

Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371

Inserts

Dendra2
Cerulean

Use

Zebrafish expression

Tags

H2B tag and membrane tag

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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PhOTO-M (pMTB:memb-Dendra2-2A-H2B-Cerulean)

Plasmid

#92401

Purpose

Constitutive expression of membrane green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.

Depositor

Periklis Pantazis

Article

Dempsey et al PLoS One. 2012;7(3):e32888. doi: 10.1371

Inserts

Dendra2
Cerulean

Use

Zebrafish expression

Tags

H2B tag and membrane tag

Expression

Mutation

Promoter

bactin2

Availability

Academic Institutions and Nonprofits only

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PhOTO-C (pMTB:cyto-Dendra2-2A-H2B-Cerulean)

Plasmid

#92402

Purpose

Constitutive expression of cytosolic green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.

Depositor

Periklis Pantazis

Article

Kalyviotis et al (2020) Behavioral and Neural Genetics of

Inserts

Dendra2
Cerulean

Use

Zebrafish expression

Tags

H2B tag and No tag, cytosolic expression

Expression

Mutation

Promoter

bactin2

Availability

Academic Institutions and Nonprofits only

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pLentiPGK Hygro DEST H2B-mCerulean3

Plasmid

#90234

Purpose

Lentiviral vector to express H2B-mCerulean3 under PGK promoter (With Hygromycin Resistance)

Depositor

Markus Covert

Article

Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

Insert

H2B

Use

Lentiviral

Tags

mCerulean3

Expression

Mammalian

Mutation

Promoter

PGK

Availability

Academic Institutions and Nonprofits only

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pDUET-1-alpha-synuclein-mCerulean3-His6

Plasmid

#110061

Purpose

For bacterial expression of human alpha-synuclein, carboxyl terminally tagged with mCerulean3 and poly-histidines

Depositor

Dan Mulvihill

Article

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Insert

alpha-synuclein (SNCA Human)

Use

Tags

6 x His and mCerulean3

Expression

Bacterial

Mutation

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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LOC5567066 Gastrin-releasing peptide receptor

Plasmid

#115372

Purpose

Expresses Ae. aegypti receptor in mammalian cells

Depositor

Leslie Vosshall

Article

Duvall et al Cell. 2019 Feb 7;176(4):687-701.e5. doi:

Insert

LOC5567066 Gastrin-releasing peptide receptor (LOC5567066 Aedes aegypti)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds

Plasmid

#119074

Purpose

Zebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporter

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

ubb:MCP-p65-HSF1-2a-Cerulean

Use

Tags

Expression

Bacterial

Mutation

Promoter

Zebrafish ubiquitin promoter

Availability

Academic Institutions and Nonprofits only

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pCDH-CAG-IntCCCre-EF1a-Cerulean

Plasmid

#160508

Purpose

Expresses IntCCCre in mammalian cells in split intein-mediated split-Cre recombinase applications

Depositor

Shawn Je

Article

Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

Insert

IntCCCre

Use

Cre/Lox and Lentiviral

Tags

mCerulean

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mCerulean 156-239-GGGS

Plasmid

#162616

Purpose

Allows for transcription of control mCerulean fluorophore half for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Article

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Insert

mCerulean

Use

Tags

Expression

Mammalian

Mutation

156-239

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MUC2 D1 with cleavable His tag

Plasmid

#181813

Purpose

Expresses human MUC2 D1 with cleavable His tag, residues 21-389

Depositor

Deborah Fass

Article

Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

Insert

Muc2 D1 (MUC2 Human)

Use

Tags

6xHistadine followed by TEV cleavage site

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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MsACR1-TS-Kv-P2A-mCerulean3-pAAV-CaMKII

Plasmid

#204970

Purpose

Soma-targeted neuronal expression of MsACR1 and mCerulean3 separated by a P2A site.

Depositor

Peter Hegemann

Article

Oppermann et al eLife 2023 (Reviewed Preprint)

Insert

MsACR1

Use

AAV

Tags

mCerulean3

Expression

Mutation

Promoter

CaMKII

Availability

Academic Institutions and Nonprofits only

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raACR-TS-Kv-P2A-mCerulean3-pAAV-CaMKII

Plasmid

#204971

Purpose

Soma-targeted neuronal expression of the MsACR1 mutant raACR and mCerulean3 separated by a P2A site.

Depositor

Peter Hegemann

Article

Oppermann et al eLife 2023 (Reviewed Preprint)

Insert

raACR

Use

AAV

Tags

mCerulean3

Expression

Mutation

Promoter

CaMKII

Availability

Academic Institutions and Nonprofits only

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Ef1a-somBiPOLES-mCerulean-IRES-Puro

Plasmid

#192585

Purpose

lentiviral vector for bidirectional optogenetic manipulation of Cre-positive neurons

Depositor

Simon Wiegert

Insert

somBiPOLES

Use

Lentiviral

Tags

soma-targeting motif from Kv2.1 channel

Expression

Mammalian

Mutation

Promoter

Ef1-alpha

Availability

Academic Institutions and Nonprofits only

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pSems-leader-mXFPm-IFNGR1(18-489)

Plasmid

#192786

Purpose

Expression of mXFPm-tagged IFNGR1 at the plasma membrane, e.g. for single molecule fluorescence microscopy

Depositor

Jacob Piehler

Article

Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

Insert

IFNGR1 (IFNGR1 Human)

Use

Tags

Ig k-chain leader sequence and mXFPm

Expression

Mammalian

Mutation

mXFPm: tryptophan 66 to phenylalanine, gluatmic a…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pSems-leader-mXFPe-IFNGR2(30-337)

Plasmid

#192787

Purpose

Expression of mXFPe-tagged IFNGR2 at the plasma membrane, e.g. for single molecule fluorescence microscopy

Depositor

Jacob Piehler

Article

Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

Insert

IFNGR2 (IFNGR2 Human)

Use

Tags

Ig k-chain leader sequence and mXFPe

Expression

Mammalian

Mutation

mXFPe: tyrosine 66 to phenylalanine, asparagine 1…

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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paavCAG-pre-mGRASP-mCerulean-2A-nls-mCherry

Plasmid

#34911

Depositor

Jinhyun Kim

Article

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Insert

presynaptic mGRASP

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_HA-p115-TEV-cleaved #957#382

Plasmid

#42107

Depositor

Ayano Satoh

Article

Koreishi et al Antibody Technology Journal. 2011;1:5-11

Insert

p115

Use

Tags

HA

Expression

Mammalian

Mutation

deleted 886 to end and added ENLYFQ

Promoter

CMV

Availability

Academic Institutions and Nonprofits only
pME-H2B-mCerulean3-P2A-mKOFP2-CAAX

Plasmid

#75154

Purpose

Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

H2B-mCerulean3-P2A-mKOFP2-CAAX

Use

Middle entry vector for multisite gateway three f…

Tags

Expression

Mutation

Promoter

n/a

Availability

Academic Institutions and Nonprofits only

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p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX

Plasmid

#75177

Purpose

Nuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

H2B-mCerulean3-P2A-mKOFP2-CAAX

Use

Three prime entry vector for multisite gateway th…

Tags

Expression

Mutation

Promoter

n/a

Availability

Academic Institutions and Nonprofits only

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