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Plasmid#190877PurposeThe plasmid of CRISPR/SpaCas12f1(Syntrophomonas palmitatica Cas12f1) for genome editing in human cellsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only
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All-in-one lenti-ABE8e-NG
Plasmid#200447PurposeLentivirus vector for base editingDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-GP
Plasmid#60495PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and puromycin resistance geneDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
SpyDock
Plasmid#124618PurposeExpresses SpyDock to bind SpyTag non-covalently for affinity purificationDepositorInsertSpyDock
UseTagsHis6ExpressionBacterialMutationE77A mutation prevents isopeptide bond formation,…PromoterT7Available sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-2xFYVE
Plasmid#140050PurposeExpresses mCherry-tagged HRS 2xFYVE in mammalian cellsDepositorInserttandem HRS FYVE domain (Hgs Mouse)
UseTagsmCherryExpressionMammalianMutationtwo FYVE domains (amino acids 147-223), linked by…PromoterCMVAvailable sinceMay 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_T2A_iRFP670 sgAAVS1
Plasmid#192477PurposeAll-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a controlDepositorInsertSpCas9
UseTagsT2A iRFP670ExpressionMammalianMutationPromoterAvailable sinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
phage-cmv-cfp-24xms2
Plasmid#40651DepositorInsertCFP-24xMBS
UseLentiviralTagsCFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
GFP-hPIKfyve
Plasmid#121148PurposeExpresses GFP-tagged human PIKfyve in mammalian cells.DepositorInsert1-phosphatidylinositol 3-phosphate 5-kinase isoform 2 (PIKFYVE Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFv_Her2-BFP_RHL027
Plasmid#164823PurposeLentiviral vector for constitutive expression of HER2+TM(23-675)-BFPDepositorInsertExtracellular and transmembrane region of ERBB2 (ERBB2 Human)
UseLentiviralTagstagBFPExpressionMutationPromoterSFFVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLBH531_MBP-Cas14a1 expression
Plasmid#112500PurposeExpresses MBP fused to Cas14a1DepositorInsertCas14a1
UseCRISPRTags10xHis-MBPExpressionBacterialMutationPromoterT7Available sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-JAG1-Fc(DAPA)-AviTag-6xHis
Plasmid#157024PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertJAG1 (JAG1 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBMCL1
Plasmid#178203PurposeAn improved vector for BacMam systemDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
TFORF2260
Plasmid#141998PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSOX17 (SOX17 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFJ350 - MCS(ttTi5605, II)
Plasmid#34866DepositorInsertttTi5605 targeting
UseWorm targetingTagsExpressionMutationPromoterAvailable sinceFeb. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
YFVdelNS1-Cre
Plasmid#179952PurposepCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) but with CreDepositorInsertYFV-17D lacking nonstructural protein 1 (NS1), but with Cre
UseTransneuronal tracingTagsExpressionMutationPromoterSP6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Non tagged Avidin
Plasmid#214835PurposeThis plasmid encodes a protein (Non tagged Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertNon tagged Avidin
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0
Plasmid#174081PurposeExpression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.DepositorInsertNES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
UseTagsNES-FlagExpressionMammalianMutationL270G, Y271L, N311G, C313APromoterCMVAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Vim-SNAP-tag2
Plasmid#226518PurposeMamalian cell expression of intermediate filaments (vimentin) fused to SNAP-tag2DepositorInsertVimentin-SNAP-tag2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN114 - pCAGGS-Tir1-V5-BpA-Frt-PGK-EM7-PuroR-bpA-Frt-Rosa26
Plasmid#92143PurposeTargeting vector to introduce an osTir1 expressing cassette at the mouse Rosa26 locus using Puromycin selection. Auxin-inducible degron system.DepositorInsertosTir1
UseMouse TargetingTagsV5 tagExpressionMammalianMutationPromoterAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pB-rtTA
Plasmid#126034PurposePiggyBac cargo vector expressing rtTA. Also confers resistance to G418.DepositorInsertrtTA3-IRES-Neo cassette cloned from Addgene Plasmid #25735
UsePiggybacTagsExpressionMammalianMutationPromoterhUbiCAvailable sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only