-
Plasmid#112085PurposeControl for NMD sensor: pCMV-3XFLAG-Fluc-betaglobinDepositorInsert3XFLAG firefly luciferase-beta-globin (HBB Human, Mouse, Photinus pyralis)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TID-HA3-KanMX6
Plasmid#227980PurposeTo insert biotin ligase, TurboID, at the C-terminus of genes; yeast BioID, KanMX6 markerDepositorInsertTurboID
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A no ccDB (w48-1)
Plasmid#17398PurposeGateway entry 1A vector, no ccDB geneDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseEntry vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBBR15.2-2H12.D11opt-scarRef
Plasmid#221150PurposecdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)DepositorInsertscdGreen2.1
mScarlet-I
UseTagsExpressionBacterialMutationN-terminus changed: starts with MSKKYGEAVIKE ...PromoterConstitutive and None (same as cdGreen2.1)Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ki67-mNeonGreen-IRESpuro2
Plasmid#183737PurposeExpresses Ki67-mNeonGreenDepositorInsertMKI67 (MKI67 Human)
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMIEG3-JunDN
Plasmid#40350DepositorInsertJun dominant negative (JUN Human)
UseRetroviralTagsHA and IRES eGFPExpressionMammalianMutationfirst 122 amino acids deletedPromoterAvailable sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
CMV-Brainbow-2.1 R
Plasmid#18723DepositorInserthrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
phOct4-EGFP
Plasmid#38776DepositorInsertOct-4 promoter (POU5F1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterhuman OCT4Available sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (AAV Retrograde)
Viral Prep#100853-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 (#100853). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven, Cre-dependent GECO1a calcium sensor. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-G-Flamp1-mut
Plasmid#188568Purposecontrol probe of G-Flamp1, insensitive to cAMPDepositorInsertG-Flamp1-mut
UseTagsHis tagExpressionMammalianMutationPromoterCAGAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-INPP5B
Plasmid#171947PurposeExpresses INPP5B in mammalian cellsDepositorInsertINPP5B (INPP5B Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWPI
Plasmid#12254Purpose2nd gen bicistronic lentiviral vector allows for simultaneous expression of a transgene and EGFP marker to facilitate tracking of transduced cells.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox and LentiviralTagsEMCV IRES-EGFP cassetteExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTP1005
Plasmid#104160PurposeBacterial expression plasmid of anti-mouse IgG2a Fc nanobody TP1129 (3x Cysteines)DepositorInsertAnti-mouse IgG2a Fc specific nanobody TP1129 (3xCysteines)
UseTags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn1-SIO-stGtACR2-FusionRed (AAV Retrograde)
Viral Prep#105677-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV_hSyn1-SIO-stGtACR2-FusionRed (#105677). In addition to the viral particles, you will also receive purified pAAV_hSyn1-SIO-stGtACR2-FusionRed plasmid DNA. Synapsin-driven, Cre-dependent, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsFusionRed (Cre-dependent)Available sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
Sirius/pRSETB
Plasmid#51956Purposebacterial expression of Sirius, a pH-insensitive and photostable ultramarine fluorescent proteinDepositorInsertSirius
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
GfaABC1D BARK D110A p2A mCherry
Plasmid#117692PurposeGfaABC1D-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for astrocyte Gaq silencingDepositorInsertBARKrgs D110A peptide
UseAAVTagsHA / FLAGExpressionMutationD110APromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
lenti sgRNA(MS2)_puro optimized backbone
Plasmid#73797Purposeoptimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 and EF1AAvailable sinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEvol-pAzFRS.2.t1
Plasmid#73546PurposetRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAGDepositorInsertspAzFRS.2.t1
pAzFRS.2.t1
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.GCaMP6m.WPRE.SV40 (AAV1)
Viral Prep#100839-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.CAG.Flex.GCaMP6m.WPRE.SV40 (#100839). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.GCaMP6m.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent GCaMP6m calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only