Addgene

We narrowed to 6,570 results for: ski

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Halo-H1DBD-H2

Plasmid

#200205

Purpose

Expression of HSF2 with HSF1 DBD

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

Halo-H1DBD-H2 (Hsf1, Hsf2 Mouse)

Use

Tags

Halo

Expression

Mammalian

Mutation

HSF1 (1-399); HSF2 (366-1554)

Promoter

EF_1alpha

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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H2DBD-H1-Halo

Plasmid

#200193

Purpose

Expression of HSF1 with HSF2 DBD

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

H2DBD-H1-Halo (Hsf1, Hsf2 Mouse)

Use

Tags

Halo

Expression

Mammalian

Mutation

HSF2 (1-366); HSF1 (399-1576)

Promoter

EF_1alpha

Available since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-pSMANTIS_SMAD-FOS_Mutant

Plasmid

#194188

Purpose

Includes the promoter (1kb) of SMTS with mutated SMAD/FOS binding site

Depositor

Matthias Leisegang, Ralf Brandes

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Tags

Expression

Mutation

mutated SMAD/FOS Site, Region 3-15nt of 1000nt

Promoter

SMANTIS promoter (1kb), mutated

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-pSMANTIS_STAT1-2_Mutant

Plasmid

#194189

Purpose

Includes the promoter (1kb) of SMTS with mutated STAT1/2 binding site

Depositor

Matthias Leisegang, Ralf Brandes

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Tags

Expression

Mutation

mutated STAT1/2 site, Region 653-673nt of 1000nt

Promoter

SMANTIS promoter (1kb), mutated

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-hSOX2AV

Plasmid

#193352

Purpose

Retroviral vector expressing human SOX2A61V point mutant

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

SOX2AV (SOX2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

SOX2A61V is a highly cooperative point mutant of …

Promoter

5'LTR

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hZBTB7B_R363L

Plasmid

#180381

Purpose

lentiviral transduction of human ZBTB7B gene with a point mutation (R363L) in the first ZF domain

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

ZBTB7B_R363L (ZBTB7B Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

R363L

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hZBTB7B_D35N_R49Q

Plasmid

#180383

Purpose

lentiviral transduction of human ZBTB7B gene with point mutations (D35N and R49Q) in the BTB domain

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

ZBTB7B_D35N/R49Q (ZBTB7B Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

D35N/R49Q

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hZBTB7B_R49H

Plasmid

#180382

Purpose

lentiviral transduction of human ZBTB7B gene with a point mutation (R49H) in the BTB domain

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

ZBTB7B_R49H (ZBTB7B Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

R49H

Promoter

CMV

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shIrak1-2

Plasmid

#180398

Purpose

Producing AAV that encodes mouse Irak1 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shIrak1-2 (Irak1 Mouse)

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CBA

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shIrak1-3

Plasmid

#180399

Purpose

Producing AAV that encodes mouse Irak1 shRNA-3 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shIrak1-3 (Irak1 Mouse)

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CBA

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shIrak1-1

Plasmid

#180397

Purpose

Producing AAV that encodes mouse Irak1 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shIrak1-1 (Irak1 Mouse)

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CBA

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-osr1-KI-donor

Plasmid

#184875

Purpose

Donor template for knock-in of p2a-CreERT2 into the medaka osr1 locus via CRISPR/Cas9-mediated homology directed repair

Depositor

Christoph Winkler

Article

Hui et al Development. 2022 May 20. pii: 275483. d

Inserts

Odd-Skipped Related Transcription Factor 1 5' homology arm
Odd-Skipped Related Transcription Factor 1 3' homology arm
p2a-CreERT2
Myosin light chain 2 promoter
EGFP

Use

CRISPR and Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pFUGW-shRIIα-RIIαS97A-IRES-EGFP

Plasmid

#183457

Purpose

Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variant

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA and Prkar2a (Prkar2a Rat)

Use

Lentiviral

Tags

Expression

Mutation

shRNA-resistant mutations: C135>G, G138>A, …

Promoter

shRNA: H1 / gene: ubiquitin

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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pFUGW-shRIIα-RIIαS97E-IRES-EGFP

Plasmid

#183458

Purpose

Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variant

Depositor

Matthew Gold

Article

Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

Insert

sgRNA and Prkar2a (Prkar2a Rat)

Use

Lentiviral

Tags

Expression

Mutation

shRNA-resistant mutations: C135>G, G138>A, …

Promoter

shRNA: H1 / gene: ubiquitin

Available since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shRps6ka2-3

Plasmid

#180390

Purpose

Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-3 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

shRps6ka2-3 (Rps6ka2 Mouse)

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CBA

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shRps6ka2-2

Plasmid

#180389

Purpose

Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

shRps6ka2-2 (Rps6ka2 Mouse)

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CBA

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shRps6ka2-1

Plasmid

#180388

Purpose

Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al EMBO J. 2021 Apr 1;40(7):e106106. doi: 1

Insert

shRps6ka2-1 (Rps6ka2 Mouse)

Use

AAV and RNAi

Tags

Expression

Mutation

Promoter

CBA

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.mArid3a.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#169318

Purpose

Doxycycline-inducible expression of murine Arid3a cDNA with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Arid3a (Arid3a Mouse)

Use

Lentiviral

Tags

IRES-dTomato

Expression

Mutation

Promoter

TRE

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.KRAB-mArid3a.IRES.GFP

Plasmid

#169306

Purpose

Constitutive overexpression of murine KRAB-Arid3a fusion protein with GFP as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

KRAB-Arid3a (Arid3a Mouse)

Use

Lentiviral

Tags

IRES-EGFP

Expression

Mutation

Promoter

SFFV

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.VP64-mArid3a.IRES.GFP

Plasmid

#169313

Purpose

Constitutive overexpression of murine VP64-Arid3a fusion protein with GFP as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

VP64-Arid3a (Arid3a Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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