-
Plasmid#108524PurposePhlFAM + PPhlF-YFP Reporter. DAPG sensor for use in non-Marionette strain.DepositorInsertPhlFAM + PPhlF-YFP
UseTagsExpressionBacterialMutationPhlF: S50G S73I R95H A121V M137I N144D T155I R200CPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKM126
Plasmid#105134PurposeClostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriTDepositorInsertsUpstream & Downstream pyrE deletion region
tetR
Cas9
gRNA
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTC217
Plasmid#70018PurposeCas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1bDepositorInsertNuclease (Cas9/sgRNA) + Donor + GVR
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLJC5-LAMP1-RFP-2xFlag
Plasmid#102931PurposeExpress a lysosomal TagDepositorInsertLAMP1
UseLentiviralTagsmRFP-2xFlagExpressionMutationPromoterubcAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
-
pBbdCas9S_P0526-sgRNAmreB
Plasmid#149654Purposeall-in-one CRISPRi vector for targeting B. burgdorferi mreBDepositorInsertdCas9, lacI, sgRNAmreB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, P0526Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST-HemmarI2
Plasmid#112815Purposegateway cloning destination vector for expressing enhancer driven CD4-IFP2.0-T2A-HO1DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2-UTY-F
Plasmid#17439PurposeExpression of UTY in mammalian cellsDepositorInsertUTY (UTY Human)
UseTags6xMyc and FlagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-dCas9-P2A-HSA
Plasmid#121936PurposeCRISPR-Sirius plasmidDepositorInsertdCas9-P2A-HSA
UseCRISPR and LentiviralTagsHSAExpressionMammalianMutationPromoterCMV-TOAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI-HA-ERK5-FL
Plasmid#31817DepositorInsertErk5 (Mapk7 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJK02
Plasmid#105133PurposeClostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriTDepositorInsertsUpstream & Downstream pyrE deletion region
tetR
Cas9
gRNA
UseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSR0
Plasmid#161984PurposeAllele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile ribotype 027 and other ribotypes. Uses inducible expression of CD2517.1 toxin as a counter-selection markerDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAJM.1642
Plasmid#108535PurposeCinRAM + PCin-YFP Reporter. OHC14 sensor for use in non-Marionette strain.DepositorInsertCinRAM + PCin-YFP
UseTagsExpressionBacterialMutationCinR: S72C V80I G114D G115D R137H K220R A224T *24…PromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-mMALAT1_3' WT Sense
Plasmid#46834PurposeExpress cGFP transcript ending in the wildtype MALAT1 triple helixDepositorInsertCoral Green Fluorescent Protein (cGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTRE2-Bla(HA–RILP)
Plasmid#102425PurposeHA tag fused to the N-terminus of RILP for the expression in mammalian cells.DepositorInsertRab interacting lysosomal protein (RILP Human)
UseTagsHAExpressionMammalianMutationPromoterTet-responsiveAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
VAMP2-pHuji
Plasmid#105289PurposeVAMP2 tagged with pH-sensitive red fluorescent protein pHujiDepositorInsertVAMP2 (Vamp2 Rat)
UseTagspHujiExpressionMammalianMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
ER-EGFP-eMagA
Plasmid#162245PurposeBlue-light dependent heterodimerization systemDepositorInsertCytP450 ER targeting sequencel-EGFP-eMagA
UseTagsExpressionMammalianMutationPromoterbeta-actin promoterAvailable sinceJan. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV-U6+27-tCorn
Plasmid#106233PurposeExpress tRNA-Corn as a reporter of U6 promoter activity in mammalian cellsDepositorInserttRNA-Corn
UseTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
EcSTH-LbNOX
Plasmid#211924PurposeThis plasmid contains human codon optimized sequence of EcSTH-LbNOX which can be used to decrease NADPH/NADP ratio in mammalian cells.DepositorInsertEcSTH-LbNOX
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet_peroxisome_C1
Plasmid#85063PurposeIn vivo visualization of the peroxisome (can be used for colocalization studies)DepositorInsertPeroxisomal targeting sequence
UseTagsmScarletExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only