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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 31817 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCI
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameErk5
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SpeciesM. musculus (mouse)
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Entrez GeneMapk7 (a.k.a. BMK-1, BMK1, ERK, ERK-5, ERK5, Erk5-, Erk5-T, PRKM7, b2b2346C, b2b2346Clo)
- Promoter CMV
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site none (unknown if destroyed)
- 3′ cloning site none (unknown if destroyed)
- 5′ sequencing primer CMV forward
- 3′ sequencing primer EBV reverse (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCI-HA-ERK5-FL was a gift from Astar Winoto (Addgene plasmid # 31817 ; http://n2t.net/addgene:31817 ; RRID:Addgene_31817) -
For your References section:
ERK5 is a novel type of mitogen-activated protein kinase containing a transcriptional activation domain. Kasler HG, Victoria J, Duramad O, Winoto A. Mol Cell Biol. 2000 Nov;20(22):8382-9. 10.1128/MCB.20.22.8382-8389.2000 PubMed 11046135