pAV-U6+27-tCorn
(Plasmid #106233)

• Purpose: Express tRNA-Corn as a reporter of U6 promoter activity in mammalian cells
• Depositing Lab: Samie Jaffrey
• Publication: Song et al Nat Chem Biol. 2017 Nov;13(11):1187-1194. doi: 10.1038/nchembio.2477. Epub 2017 Sep 25.

Backbone

• Vector backbone: pAV U6+27
• Backbone manufacturer: Addgene plasmid # 25709
• Backbone size w/o insert (bp): 6092
• Total vector size (bp): 6252
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: tRNA-Corn
• Alt name: tCorn
• Species: Synthetic
• Insert Size (bp): 160
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Sal1 (not destroyed)
• 3′ cloning site: Xba1 (not destroyed)
• 5′ sequencing primer: TGACACACATTCCACAGCC
• 3′ sequencing primer: CGGCCGCCATATGGATATC

Resource Information

• Supplemental Documents:
  • pAVU6+27-tCorn.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry