Skip to main content
Addgene
Showing: 4981 - 5000 of 11161 results

  3. pBetaActin-FKBP-mScarlet-Dync1h1motorK2599T

    Plasmid
    #191331
    Purpose
    Expresses the fluorescently labeled motor-deficient dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    FKBP-mScarlet-Dync1h1motorK2599T (Dync1h1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    DYNC1H1 motor domain aa 1453-4644
    Promoter
    Available since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    191331-plasmid-map-sequence-id-381751

    Enlarge

  4. TFORF0795

    Plasmid
    #141703
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ATF7 (ATF7 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141703-plasmid-map-sequence-id-404524

    Enlarge

  5. TFORF0362

    Plasmid
    #141589
    Purpose
    Lentiviral vector for overexpressing the TCEB2 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TCEB2 (ELOB Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141589-plasmid-map-sequence-id-409098

    Enlarge

  7. ATX3-D1

    Plasmid
    #184246
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain and UIM1 fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    Ataxin-3 Josephin domain and UIM1 (ATXN3 Human)
    Use
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Premature stop codon N263X
    Promoter
    T7 promoter
    Available since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184246-plasmid-map-sequence-id-370314

    Enlarge

  11. pET24TEV-tSHIP2

    Plasmid
    #183771
    Purpose
    Expresses C-terminal truncated SHIP2 (tSHIP2) with C-terminal TEV and 6HIS tags from pET24 backbone
    Depositor
    William Kerr
    Article
    Sudan et al Front Immunol. 2022 May 4;13:830961. doi
    Insert
    SHIP2 (INPPL1 Human)
    Use
    Tags
    6HIS and Tobacco Etch Virus (TEV) Cleavage site
    Expression
    Bacterial
    Mutation
    includes aa18 to aa985 only
    Promoter
    T7 lac
    Available since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    183771-plasmid-map-sequence-id-362691

    Enlarge

  14. pDONR_P4-P1R:PER36modp

    Plasmid
    #128431
    Purpose
    Gateway (Invitrogen) promoter clone (pDONR_P4-P1R) with premature stop codons introduced within the Xero1 and Xero2 genes in the PER36 promoter entry clone (Kunieda et al. 2013).
    Depositor
    George Haughn
    Article
    McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100
    Insert
    PEROXISADE36 (AT3G50990 Mustard Weed)
    Use
    Gateway promoter entry clone
    Tags
    Expression
    Mutation
    Premature stop codons introduced into the Xero1 (…
    Promoter
    PEROXISADE36
    Available since
    Oct. 4, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    128431-plasmid-map-sequence-id-257603

    Enlarge

  15. 289aa ELL2

    Plasmid
    #127266
    Purpose
    For in vitro translation of shorter human ELL2 from Met1. Also contains Met133I, M1381I, M186I mutations.
    Depositor
    Christine Milcarek
    Article
    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi
    Insert
    NH2-ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
    Use
    In vitro translation
    Tags
    Expression
    Mutation
    Three Mets (133, 138, 186) to Ileu. Contains 289…
    Promoter
    T7
    Available since
    July 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    127266-plasmid-map-sequence-id-250216

    Enlarge

  17. HA tagged Sph ELL2 Met133, 138, 186 to Ileu

    Plasmid
    #127271
    Purpose
    For in vitro translation of human ELL2 with Met133, 138, 186 to Ileu with HA tag after 8th Met
    Depositor
    Christine Milcarek
    Article
    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi
    Insert
    ELL2 Met133, 138, 186 to Ileu (ELL2 Human)
    Use
    In vitro translation
    Tags
    Expression
    Mutation
    Met133, 138, 186 to Ileu with HA tag after 8th Met
    Promoter
    T7
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127271-plasmid-map-sequence-id-250013

    Enlarge

  18. HA tagged mid ELL2 M133, 138, 186ILeu

    Plasmid
    #127276
    Purpose
    Expresses human ELL2 with M133, 138, 186ILeu to prevent internal Met initiation peptides and contains middle HA tag that doesn't disrupt function
    Depositor
    Christine Milcarek
    Article
    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi
    Insert
    ELL2 (ELL2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    M133, 138, 186 to Ileu, HA tag at XbaI after Met …
    Promoter
    pEF1a
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127276-plasmid-map-sequence-id-249998

    Enlarge

  19. EF1a_MYC Glu39Ala_P2A_Hygro_Barcode

    Plasmid
    #120512
    Purpose
    Barcoded lentiviral vector to express MYC Glu39Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.
    Depositor
    Prashant Mali
    Article
    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(
    Insert
    MYC Glu39Ala (MYC Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Point mutation changing Glutamic Acid to Alanine …
    Promoter
    EF1a
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    120512-plasmid-map-sequence-id-234964

    Enlarge

  20. N295A (EcN), E166Q (EcN) MetNI LmH

    Plasmid
    #118269
    Purpose
    N295A and E166Q Mutation of MetN in Methionine ABC Transporter with 6x His Tag. Used to solve crystal structure
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetI (metI Escherichia coli)
    MetN (E166Q ; N295A) (metN Escherichia coli)
    Use
    Tags
    6x His Tag
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 166 to Glutamine (Gln…
    Promoter
    T7 Promoter
    Available since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118269-plasmid-map-sequence-id-228542

    Enlarge

Showing: 4981 - 5000 of 11161 results