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MSCV-PIG-TAF4(827-909)

Plasmid

#105616

Purpose

retrovirally express express mouse TAF4 HFD with GFP marker and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF4 HFD-827-909 (Taf4 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ARAF_D429A

Plasmid

#51080

Purpose

mammalian expression of ARAF D429A

Depositor

Matthew Meyerson

Article

Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

Insert

ARAF (ARAF Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

D429A

Promoter

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-BAIAP2 wt

Plasmid

#31675

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

BAI1-associated protein 2 (BAIAP2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pDY1173_ADAR1p150

Plasmid

#193192

Purpose

Express full length ADAR1p150

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

ADAR1p150 (ADAR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-KRAB-dCas9-P2A-mCherry

Plasmid

#60954

Purpose

2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

Depositor

Jonathan Weissman

Article

Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

Insert

KRAB-dCas9-P2A-mCherry fusion

Use

CRISPR and Lentiviral

Tags

2x NLS, HA, KRAB domain, and mCherry

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only

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pW34

Plasmid

#158210

Purpose

LuxCDABE operon (R6K ori) under T7, in loxP and lox5171 sites.

Depositor

Yasuo Yoshikuni

Article

Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

Insert

LuxABCDE

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP

Plasmid

#60955

Purpose

Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

Depositor

Jonathan Weissman

Article

Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

Inserts

sgGFP-NT2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3-AUP1-FLAG

Plasmid

#185336

Purpose

Mammalian expression of AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Use

Tags

FLAG, 6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA AXL

Plasmid

#105932

Purpose

Mammalian expression of myc-His tagged AXL

Depositor

Rosa Marina Melillo

Article

Krishnamoorthy et al J Biol Chem. 2013 Jun 14;288(24):17481-9

Insert

AXL (AXL Human)

Use

Tags

His and myc

Expression

Mammalian

Mutation

Promoter

tet-inducible CMV

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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Rab8WT(Flag) in pcDNA3.1neo

Plasmid

#46783

Purpose

expression of WT Rab8a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

RAB8A (RAB8A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pT7-7 aSyn C141

Plasmid

#108866

Purpose

Expresses human WT alpha synuclein with a C-terminal cysteine

Depositor

Gabriele Kaminski Schierle

Article

Stephens et al bioRxiv 2022.10.04.510646

Insert

alpha synuclein (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

C141 mutation to allow C-terminal maleimide dye l…

Promoter

T7

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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Braf

Plasmid

#40775

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

Braf (BRAF Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Starting Met removed

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-LivePAR-Hygro

Plasmid

#176063

Purpose

EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

LivePAR

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pDY1462_SERPINA1_CCA32_Caspase

Plasmid

#193194

Purpose

Express Caspase conditional upon RNA transcript

Depositor

Jonathan Gootenberg, Omar Abudayyeh

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

iCaspase9 (CASP9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Chr7_Centromere-Targeting_gRNA

Plasmid

#195129

Purpose

dual gRNA vector targeting centromere-proximal locations on Chromosome 7p and 7q in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr7 gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Chr8q_Centromere-Targeting_gRNA

Plasmid

#195128

Purpose

gRNA targeting centromere-proximal location on Chromosome 8q in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr8q gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Chr1q_Centromere-Targeting_gRNA

Plasmid

#195125

Purpose

gRNA targeting centromere-proximal location on Chromosome 1q in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr1q gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV-Neo-GFP/empty

Plasmid

#105505

Purpose

Empty backbone of MSCV-driven retroviral cDNA expression

Depositor

Christopher Vakoc

Article

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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h-Plekhm1_FLAG-pcDNA 3.1

Plasmid

#73593

Purpose

Express human Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

Insert

Plekhm1 (PLEKHM1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pECE-M2-BAIAP2 wt

Plasmid

#31656

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

BAI1-associated protein 2 (BAIAP2 Human)

Use

Tags

M2

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only