-
Plasmid#79469PurposesgRNA shuttle vector; module 5DepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE13
Plasmid#79471PurposesgRNA shuttle vector; module 6DepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE14
Plasmid#79473PurposesgRNA shuttle vector; module 7DepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE15
Plasmid#79474PurposesgRNA shuttle vector; module 8EDepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE10
Plasmid#79466PurposesgRNA shuttle vector; module 4DepositorInsertpAtU6-ccdB-sgRNA (ccdB(letD) )
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTd-4-15-13-10
Plasmid#63835PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
UseTagsExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pReporter_3
Plasmid#60564PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_3
UseTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pChlr-8CQ
Plasmid#52171PurposeEncodes module 8 with amino acids 12C-16Q for recognition of nt 1ADepositorInsertPumilio homology domain amino acids 284-319 (PUM1 Human)
UseTagsExpressionBacterialMutationChanged 12N to 12C in module 8PromoterAvailable sinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
-
pReporter_12
Plasmid#60572PurposeA reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).DepositorInsertReporter_12
UseTagsExpressionBacterialMutationPromoterBBa_J23119Available sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB310
Plasmid#68638PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTags3xFLAGExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM843
Plasmid#84169Purposetarget miRNA843 for destruction in Arabidopsis thalianaDepositorInsertSTTM843
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM845
Plasmid#84170Purposetarget miRNA845 for destruction in Arabidopsis thalianaDepositorInsertSTTM845
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDEST-APIC-Cas9
Plasmid#121657Purposegateway cloning destination vector for expressing enhancer driven Cas9DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB212
Plasmid#68603PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsGlucocorticoid receptor (GR)ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZA-mCherry-R5
Plasmid#106258PurposeExpresses mCherry fused with silaffin-R5 silica affinity peptide under the control of TetODepositorInsertmCherry
UseTagsAdded silaffin R5 peptide to C terminus of mCherr…ExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-006
Plasmid#78693PurposeA weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAF257
Plasmid#105497PurposepRPF185 derived plasmid encoding an anhydrotetracyclin inducible SmBiT and HupA-LgBiTDepositorInsertSmBiT/HupA-LgBiT
UseAtc-dependent expression in c. difficileTagsExpressionMutationPromoterPtetAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFPL-Yh2
Plasmid#61650PurposeGateway vector for generating yellow fluorescent protein fusions in fungiDepositorInsertenhanced yellow fluorescent protein
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST-actc1b-ccdb-mKate2
Plasmid#109731PurposeMultisite gateway destination vector for muscle specific expression of constructs tagged at the 3' end with mKate2DepositorTypeEmpty backboneUseZebrafish plasmidsTagsExpressionMutationPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only