Addgene

We narrowed to 6,499 results for: mag

Showing: 4701 - 4720 of 6499 results

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pAAV-syn-jYCaMP1

Plasmid

#135423

Purpose

Yellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct transfection

Depositor

Kaspar Podgorski

Article

Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

Insert

jYCaMP1

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-SomArchon-P2A-CoChR-Kv2.1

Plasmid

#126945

Purpose

Expression of SomArchon-P2A-CoChR-Kv2.1 under Syn promoter

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon-P2A-CoChR-Kv2.1

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

June 24, 2019

Availability

Academic Institutions and Nonprofits only

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pELPS-eDHFR-YFP-T2A-Renilla

Plasmid

#193253

Purpose

Triple reporter plasmid in pELPS with eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase

Depositor

Mark Sellmyer

Article

Sellmyer et al Mol Ther. 2020 Jan 8;28(1):42-51. doi: 1

Insert

The eDHFR (PET reporter gene) fused to yellow fluorescent protein (YFP) and a T2A cleavage site followed by Renilla luciferase

Use

Lentiviral

Tags

Renilla luciferase (rLuc) and yellow fluorescent …

Expression

Mutation

None

Promoter

EF1a

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-eGFP-NLS-RNF168

Plasmid

#133977

Purpose

mammalian expression vector of eGFP tagged wild type RNF168

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

siRNA resistant sequence: GAGGAGTCGTGTTTATTGA

Promoter

CMV

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-BLM DM

Plasmid

#80071

Purpose

Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations

Depositor

Nathan Ellis

Article

Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-6

Insert

BLM (BLM Human)

Use

Tags

GFP-BLM

Expression

Mammalian

Mutation

K317R (AAA>AGA) and K331R (AAA>AGA)

Promoter

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-dL5**-mCER-TRF1

Plasmid

#168176

Purpose

Expresses FAP (dL5**) fused to mCerulean and the telomere binding protein TRF1

Depositor

Patricia Opresko

Article

Fouquerel et al Mol Cell. 2019 Jul 11;75(1):117-130.e6.

Insert

TRF1 (TERF1 Human)

Use

Lentiviral

Tags

FAP (dL5**) and mCerulean

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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hRAD18 dC2-EGFP

Plasmid

#68826

Purpose

Expresses human RAD18 (deleting Polymerase eta binding domain) tagged with EGFP

Depositor

Satoshi Tateishi

Article

Tsuji et al Genes Cells. 2008 Apr;13(4):343-54. doi:

Insert

human RAD18 (deleting Polymerase eta binding domain) tagged with EGFP (RAD18 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
ATG-3925 (CBG2)

Plasmid

#167839

Purpose

Expresses CBG2 (Click Beetle Green 2) in E. coli and mammalian cells

Depositor

Lance Encell

Article

Zambito et al iScience. 2020 Dec 26;24(1):101986. doi:

Insert

Click Beetle Green 2

Use

Tags

6-His

Expression

Bacterial and Mammalian

Mutation

Q348H. Click Beetle Green 2 (CBR2) has 3 amino a…

Promoter

CMV, T7

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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tetO-GATA6

Plasmid

#170688

Purpose

doxycycline-inducible overexpression of GATA6

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

GATA6 (GATA6 Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-RNF51

Plasmid

#126546

Purpose

Expresses GFP-tagged RNF51 in mammalian cells

Depositor

Michele Pagano

Article

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

RNF51 (BMI1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

LTR

Available since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1/PD-L1_ICD

Plasmid

#121480

Purpose

expression of human PD-L1 in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

TM+ICD

Promoter

CMV

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1/PD-L1_ECD

Plasmid

#121479

Purpose

expression of human PD-L1 in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

ECD+TM

Promoter

CMV

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pSEMS-Tom20-mEGFP-reHaloTagS

Plasmid

#200189

Purpose

Tom20 with reversible HaloTagS variant for fluorescent labeling

Depositor

Jacob Piehler

Article

Holtmannspotter et al Angew Chem Int Ed Engl. 2023 Feb 3:e2022

Insert

Tom20 (TOMM20 Human)

Use

Tags

mEGFP and reHaloTagS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pNK3203

Plasmid

#219749

Purpose

DVK_AF CIDAR vector encoding hispidin-synthase from Mycena citricolor under control of CMV promoter, for mammalian expression

Depositor

Alexander Mishin

Article

Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

Insert

pCMV - mcitHispS - tSV40

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-miRFP-6xFLAG

Plasmid

#161614

Purpose

Cell morphology marker construct. Contains a near infrared fluorescent protein miRFP and six FLAG tags.

Depositor

Edward Boyden

Article

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

miRFP-6xFLAG

Use

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Human synapsin 1 promoter

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mRuby3-6xFLAG

Plasmid

#161613

Purpose

Cell morphology marker construct. Contains a red fluorescent protein mRuby3 and six FLAG tags.

Depositor

Edward Boyden

Article

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

mRuby3-6xFLAG

Use

Tags

Expression

Mammalian

Mutation

N/A

Promoter

Human synapsin 1 promoter

Available since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-SECFP-Galectin3

Plasmid

#64150

Purpose

Expression in mammalian cells (production of retrovirus vector)

Depositor

Tamotsu Yoshimori

Article

Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

Insert

Galectin3 (LGALS3 Human)

Use

Retroviral

Tags

SECFP

Expression

Mammalian

Mutation

Promoter

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pACEBac1-PARP1-Flag-His-7xKQ

Plasmid

#111573

Purpose

Expresses full length PARP1 with K to Q mutations in automodification domain in Sf9 (insect) cells

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP1 (PARP1 Human)

Use

Tags

C-terminal 6xHis and C-terminal Flag

Expression

Insect

Mutation

lysine to glutamine mutation at the following ami…

Promoter

polyhedrin promoter

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pD2sE_SC10

Plasmid

#219716

Purpose

DV2 stable E dimer with fusion loop mutation

Depositor

Brian Kuhlman

Article

Kudlacek et al Sci Adv. 2021 Oct 15;7(42):eabg4084. doi

Insert

DV2 sE SC10

Use

Tags

8xHis and human serum albumin (HSA)

Expression

Mammalian

Mutation

G106D, A259W, T262R, F279W, T280P

Promoter

Available since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pDB-hPER2-mScarlet-I-CD4-bla

Plasmid

#179449

Purpose

Donor vector to knock in mScarlet-I C-terminal to human PER2 gene

Depositor

Achim Kramer

Article

Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

Insert

mScarletI

Use

CRISPR

Tags

His/Flag

Expression

Mutation

Promoter

None

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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