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Showing: 1 - 20 of 5979 results

  3. pCMVMagFRET-1

    Plasmid
    #50742
    Purpose
    Expresses MagFRET-1 in the cytosol of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal cytosolic Ca2+ levels.
    Depositor
    Maarten Merkx
    Article
    Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:
    Insert
    gene encoding for MagFRET-1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    50742-plasmid-map-sequence-id-327713

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  4. pORTMAGE-4

    Plasmid
    #72679
    Purpose
    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Cam resistance marker.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    Available since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    72679-plasmid-map-sequence-id-198908

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  5. pORTMAGE-2

    Plasmid
    #72677
    Purpose
    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Ap resistance marker.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    pL promoter
    Available since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    72677-plasmid-map-sequence-id-451033

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  7. pORTMAGE-3

    Plasmid
    #72678
    Purpose
    Expresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    Available since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    72678-plasmid-map-sequence-id-198909

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  8. pORTMAGE311B

    Plasmid
    #120418
    Purpose
    Expresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanR
    Depositor
    Csaba Pál
    Article
    Szili et al bioRxiv (2018)
    Inserts
    MutL E32K
    Lambda Beta
    XylS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    Pm
    Available since
    Feb. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    120418-plasmid-map-sequence-id-236013

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  12. pORTMAGE-1

    Plasmid
    #72680
    Purpose
    Same as pORTMAGE-2, but with a non-optimized RBS before mutant MutL, thus lower level of expression of the enzyme.
    Depositor
    Csaba Pál
    Article
    Nyerges et al Proc Natl Acad Sci U S A. 2016 Feb 16. p
    Insert
    mutL E32K
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    lower strength RBS before mutL E32K allele than i…
    Promoter
    Available since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only

  15. MAGEA4

    Plasmid
    #39001
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAGEA4 (MAGEA4 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    39001-plasmid-map-sequence-id-218943

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  16. pORTMAGE502B

    Plasmid
    #128971
    Purpose
    Broad-host-range GenR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid induction
    Depositor
    George Church
    Inserts
    PapRecT
    mutL E32K
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E32K
    Promoter
    xyl-Pm
    Available since
    Aug. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    128971-plasmid-map-sequence-id-252654

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  17. MAGEA3

    Plasmid
    #73246
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAGEA3 (MAGEA3 Human)
    Use
    Tags
    His6-Thioredoxin-TEV
    Expression
    Bacterial
    Mutation
    pfam01454:MAGE 116-286
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73246-plasmid-map-sequence-id-137200

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  18. pORTMAGE302A

    Plasmid
    #128970
    Purpose
    Broad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with high temperature induction
    Depositor
    George Church
    Inserts
    PapRecT
    mutL E32K
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E32K
    Promoter
    cI-pL
    Available since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    128970-plasmid-map-sequence-id-252505

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Showing: 1 - 20 of 5979 results