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Plasmid#185331PurposeBacterial expression of AUP1 with GST fusion protein for binding assaysDepositorInsertAUP1 (AUP1 Human)
UseTagsGSTExpressionBacterialMutationaa 292-410 onlyPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-f:PGC1-3D
Plasmid#47DepositorInsertPGC-1a (Ppargc1a Mouse)
UseTagsflagExpressionMammalianMutationAmino acids 262, 265, and 298 have been changed t…PromoterAvailable sinceOct. 4, 2005AvailabilityAcademic Institutions and Nonprofits only -
pET21-10XHis-GST-HRV-Her2-dL5-Her2
Plasmid#85624PurposeBacterial expression vector for Z342-dL5(E52D)-Z342 affibody fusion protein (AFA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP, AffiFAP)DepositorInsert10XHis-GST-HRV-Her2-dL5-Her2 (EGFR Human, Synthetic, Schistosoma japonicum)
UseTags10XHis-GST-HRV and The Her2 Affibody and dL5 FAP …ExpressionBacterialMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterT7Available sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX/PD-L1 3SA
Plasmid#121467PurposeExpression of GST-human PD-L1 in E. ColiDepositorInsertCD274 (CD274 Human)
UseTagsGSTExpressionBacterialMutationS176A, T180A, S184APromoterTacAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hARdelta120-472
Plasmid#89088Purposemammalian expression of human androgen receptor with deletion of 120-472 amino acid residuesDepositorInserthAR-120-472 N-term deletion (AR Human)
UseTagsExpressionMammalianMutationhAR-120-472PromoterCMVAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV TIP60 1-211
Plasmid#78794PurposeTo overexpress TIP60 1-211 in Mammalian CellsDepositorInsertTIP60 (KAT5 Human)
UseTags3xFLAGExpressionMammalianMutation1-211PromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRRLHygro-pEF1a-p53ash-mKate2-splitmVenusC
Plasmid#69583PurposeExpresses p53 tagged with mKate2 and split C-term mVenusDepositorInsertp53 (TP53 Human)
UseLentiviralTagsmKate2 and split mVenus - C termExpressionMammalianMutation7 silent mutations in p53 DNA to prevent targetin…PromoterEF1alphaAvailable sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2 ECD-MHC
Plasmid#113957Purposemammalian expression plasmid for FLAG-tagged human T1R2 ECD with signal peptide of influenza hemagglutinin fused to a canonical transmembrane domain from MHC class IDepositorInsertT1R2 (TAS1R2 Human)
UseTagsFLAG, Signal/leader sequence from influenza hemag…ExpressionMammalianMutationresidues 22-568 fused to a transmembrane tetherPromotercmvAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-f:PGC1-L2/3A
Plasmid#48DepositorInsertPGC-1a (Ppargc1a Mouse)
UseTagsflagExpressionMammalianMutationLXXLL at aa140 is changed to AXXLL; LLXXL at aa 2…PromoterAvailable sinceOct. 4, 2005AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG1_Arg322Lys
Plasmid#105849PurposeExpression plasmid coding for mutated heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. Mutation: Arg322Lys (EU numbering).DepositorInsertIgG1 heavy chain (Ighg1 Mouse)
UseTagsExpressionMammalianMutationArg322Lys in constant region of IgG1 heavy chainPromoterhEF1-HTLVAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMS04 [myo-3p::bPGC::SL2::mCherry]
Plasmid#168172PurposeExpression of bPGC in BWMs of C. elegansDepositorInsertbPGC
UseTagsExpressionWormMutationPromoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA p21 K161R, K163R
Plasmid#78789PurposeTo overexpress p21 K161R, K163R in Mammalian CellsDepositorInsertp21 (CDKN1A Human)
UseTagsHAExpressionMammalianMutationK161R, K163R_muationPromoterCMVAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/BirA/FLAG-DDX54
Plasmid#97063PurposeExpresses BirA/FLAG-tagged DDX54 in mammalian cellsDepositorInsertDDX54 (DDX54 Human)
UseTagsBirA and FLAGExpressionMammalianMutationPromotertetO2Available sinceJuly 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPBbsr2-Gnaq
Plasmid#170298PurposeEncoding mouse GnaqDepositorInsertA recombinant mouse Gnaq (Gnaq Mouse)
UseTagsN/AExpressionMammalianMutationAsilent mutation was introduced into the sgRNA-ta…PromoterAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB-hCRY1-SNAP-CD4-bla
Plasmid#179447PurposeDonor vector to knock in SNAPtag C-terminal to human CRY1 geneDepositorInsertSnapTag
UseCRISPRTagsHis/FlagExpressionMutationPromoterNoneAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-hDDB2-R273H-V5
Plasmid#167469Purposeectopic expression of hDDB2 in mammalian cellsDepositorInsertDDB2 (DDB2 Human)
UseTags6xHis and V5ExpressionMammalianMutationR273HPromoterCMVAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-MAPTau-N-10
Plasmid#55498PurposeLocalization: Microtubules, Excitation: 462, Emission: 492DepositorInsertMAPTau (MAPT Human)
UseTagsmTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCS3-MT-BX-6MYC p21
Plasmid#78780PurposeTo overexpress p21 in Mammalian CellsDepositorInsertp21 (CDKN1A Human)
UseTags6xMYCExpressionMammalianMutationPromoterCMV IE94Available sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCardinal-H3-23
Plasmid#56163PurposeLocalization: Nucleus/Histones, Excitation: 604, Emission: 659DepositorInsertH3 (H3C2 Human)
UseTagsmCardinalExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCardinal-Vimentin-7
Plasmid#56178PurposeLocalization: Intermediate Filaments, Excitation: 604, Emission: 659DepositorInsertVimentin (VIM Human)
UseTagsmCardinalExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits