Addgene

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pcDNA5FRT/Flag-OGT1

Plasmid

#29760

Depositor

Joan Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

OGT1 (OGT Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

N764D and K1010E

Promoter

Available since

July 3, 2013

Availability

Academic Institutions and Nonprofits only

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Nsp3 -mCherry

Plasmid

#165131

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp3 (ORF1ab codon optimized SARS-COV-2)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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RCAS GFP (CT#22)

Plasmid

#13878

Depositor

Connie Cepko

Article

Chen et al Dev Biol. 1999 Oct 15. 214(2):370-84.

Insert

GFP

Use

Retroviral; Avian expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pBMN YFP-FKBP(DD)

Plasmid

#31766

Depositor

Thomas Wandless

Article

Chu et al Bioorg Med Chem Lett. 2008 Nov 15;18(22)

Insert

HA-YFP-FKBP(DD) (FKBP1A Human)

Use

Retroviral

Tags

HA and YFP

Expression

Mutation

E31G, F36V, R71G and K105E on FKBP12

Promoter

LTR

Available since

Oct. 4, 2011

Availability

Academic Institutions and Nonprofits only

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pMo168

Plasmid

#27389

Depositor

Martin Voskuil

Article

Hamad et al Gene. 2009 Feb 1. 430(1-2):123-31.

Insert

pMo168

Use

Tags

Expression

Bacterial

Mutation

A replicative vector for gene expression in Burkh…

Promoter

Available since

March 7, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-HA-BACE1

Plasmid

#165032

Purpose

HA-BACE1

Depositor

Juan Bonifacino

Article

Prabhu et al Mol Biol Cell. 2012 Jun;23(12):2339-51.

Insert

BACE1 (BACE1 Human)

Use

Tags

HA (embedded after propeptide cleavage site)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pScI_dCas9-CDA_J23119-sgRNA

Plasmid

#108550

Purpose

Bacterial Target-AID vector with sgRNA

Depositor

Akihiko Kondo

Article

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Inserts

dCas9-PmCDA1
Streptococcus pyogenes sgRNA

Use

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

J23119 and lambda OR

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

Plasmid

#85008

Purpose

For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

EGFP-ires-tTA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pGfaABC1D-nLac

Plasmid

#53131

Purpose

expression of lacZ driven by human GfaABC1D promoter

Depositor

Michael Brenner

Article

Lee et al Glia. 2008 Apr;56(5):481-93. doi: 10.100

Insert

Gfa2 (GFAP Human)

Use

Mouse Targeting

Tags

LacZ, NLS, and mP1

Expression

Mammalian

Mutation

contains ABC1D regions of Gfa2 promoter, -1757 to…

Promoter

human Gfa2

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV asyn WT

Plasmid

#36055

Depositor

Hilal Lashuel

Article

Oueslati et al J Neurosci. 2012 Feb 1;32(5):1536-44.

Insert

alpha-synuclein WT (SNCA Human)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pHLS-EF1a-FRB-SpCas9-A

Plasmid

#138477

Purpose

Expresses FRB fused SpCas9 for NanoMEDIC packaging.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

SpCas9, human codon optimized

Use

CRISPR

Tags

3x HA tag and FRB

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EFNB2-RBD-Fc (WT)

Plasmid

#200978

Purpose

Mammalian expression plasmid for soluble EFNB2 (wild type) fused to IgG1 Fc

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EFNB2 (EFNB2 Human)

Use

Tags

IgG1 Fc

Expression

Mammalian

Mutation

Soluble Receptor-Binding Domain

Promoter

CMV

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pRT498-OtUBD

Plasmid

#190089

Purpose

Expresses a single OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

Depositor

Mark Hochstrasser

Article

Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

Insert

OtUBD

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pK036.TRE-Flpe-WPRE (Supernova)

Plasmid

#85007

Purpose

For Flpe/FRT-based Supernova, pK036 should be used with pK068 etc.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

NLS-FLPe-WPRE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pAC-VIOL

Plasmid

#53087

Purpose

Produces the carotenoid violaxanthin in E. coli.

Depositor

Francis X Cunningham Jr

Article

Neuman et al Plant J. 2014 Apr;78(1):80-93. doi: 10.1

Insert

zep (ABA1 Mustard Weed)

Use

Low copy number bacterial cloning vector

Tags

Expression

Mutation

Lacks codons for the first 60 N-terminal amino ac…

Promoter

T7

Available since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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CALM-pmCherryN1

Plasmid

#27691

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

CALM (Picalm Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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pOpen-Tthlig

Plasmid

#165569

Purpose

Taq DNA Ligase

Depositor

FreeGenes Project, Jennifer Molloy, Drew Endy

Insert

Tth DNA Ligase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pRT498-OtUBD(V203D)

Plasmid

#190090

Purpose

Expresses OtUBD(V203D) mutant that is unable to bind ubiquitin with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

Depositor

Mark Hochstrasser

Article

Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

Insert

OtUBD(V203D)

Use

Tags

6xHis and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_ETV6_NTRK3

Plasmid

#205817

Purpose

Express mEGFP-tagged fusion protein, ETV6_NTRK3 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

ETV6_NTRK3 (ETV6, NTRK3 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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FCHo2-pmCherryC1

Plasmid

#27686

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

FCHo2 (Fcho2 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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