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pLVX-Puro-TDP-43-M337V

Plasmid

#133759

Purpose

Lentiviral expression of human TDP-43 M337V disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TDP-43 (TARDBP Human)

Use

Lentiviral

Expression

Mammalian

Mutation

Changed methionine 337 to valine

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-hMYC

Plasmid

#20723

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

Insert

c-MYC (MYC Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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plex_307-ccdB-hygro

Plasmid

#201143

Purpose

pLEX_307 plasmid with hygro marker

Depositor

Alejandro Chavez

Type

Empty backbone

Expression

Mammalian

Promoter

EF-1α

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pTRE3G-Bi-α-chain

Plasmid

#133730

Purpose

Expresses the gePSI-α-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.

Depositor

Erin Schuman

Article

Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Inserts

a-Chain_gePSI
ZsGreen1

Expression

Mammalian

Mutation

Only one part of the gePSI-system (aa1-141 + stop…

Promoter

pTRE3G-Bi

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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TCARZ-CMV-GFP-PAmin-GF3-R4A22

Plasmid

#203768

Purpose

For production of template RNA for use in PRINT, GFP under CMV promoter

Depositor

Kathleen Collins

Article

Zhang et al Nat Biotechnol. 2024 Feb 20. doi: 10.103

Insert

PRINT template RNA for GFP

Use

Ivt template

Promoter

CMV

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-dSa-VPR mini.-syn pA

Plasmid

#99689

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains with syn pA (poly adenylation) signal

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Mutation

dead Cas9

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT(EF1a)

Plasmid

#172326

Purpose

LV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

monomeric nuclear Tomato
d2eGFP
HygR

Use

Lentiviral and Synthetic Biology

Tags

3x SV40 NLS

Expression

Mammalian

Promoter

EF1-alpha

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pH2B-miRFP703

Plasmid

#80001

Purpose

Histone 2B labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

histone cluster 1 H2B family member j (H2BC11 Human)

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PRPF3

Plasmid

#155848

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PRPF3 (PRPF3 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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mOrange-N1

Plasmid

#54499

Purpose

Localization: N1 Cloning Vector, Excitation: 548, Emission: 562

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Type

Empty backbone

Tags

mOrange

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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Lyso-GA

Plasmid

#209874

Purpose

Dimerization dependent fluorescent protein GA anchored to cytosolic face of the lysosomal membrane with human LAMP1

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddGFP A

Tags

LAMP1

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pGEMT-PTE2A

Plasmid

#111773

Purpose

Quadcistronic construct: Co-express four genes by three 2A peptides -- P2A, T2A and E2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Type

Empty backbone

Use

Cloning intermediate

Promoter

T7

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221-FURIN

Plasmid

#158442

Purpose

Gateway Cloning compatible entry vector for the human FURIN gene.

Depositor

Alejandro Chavez, Sho Iketani

Insert

FURIN (FURIN Human)

Use

Gateway entry vector

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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CTCF-mAC donor (Neo)

Plasmid

#140645

Purpose

CTCF tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

CTCF (CTCF Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pTomatoSlicer

Plasmid

#221085

Purpose

Dual-color splicing reporter expressing eGFP and tdTomato from the CMV early enhancer/chicken ß-actin (CAGGS) promoter.

Depositor

Chaolin Zhang

Article

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

Aug. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCAIP-hXRCC1wt-eGFP

Plasmid

#206032

Purpose

Mammalian expression of hXRCC1wt coupled to eGFP under the control of a CAG promoter

Depositor

Primo Schaer

Article

Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

Insert

hXRCC1

Tags

eGFP

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4

Plasmid

#193345

Purpose

tetO-OS*K (Dox-inducible reprogramming lentiviral OSK vector expressing mouse Oct4 + super-Sox + Klf4)

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

Oct4-p2a-Sox2-17-t2a-Klf4 (Klf4, Pou5f1, Sox2 Mouse)

Use

Lentiviral

Expression

Mammalian

Mutation

Sox2-17 is engineered highly cooperative chimeric…

Promoter

TetO mini CMV

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLenLox_U6 p53

Plasmid

#59360

Purpose

Lentiviral expression vector: p53 shRNA under control of mouse U6 promoter, EGFP-expression driven by CAG promoter to monitor expression.

Depositor

Peter Scheiffele

Article

Chih et al Science. 2005 Feb 25;307(5713):1324-8. E

Inserts

shRNA p53 (mouse) (Trp53 Mouse)
eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CAG and mouse U6

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV10-mApg16L

Plasmid

#24302

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Sci. 2003 May 1. 116(Pt 9):1679-8

Insert

autophagy related genes 16L (Atg16l1 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

V583M

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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pBAD/myc-his A Rv0712 (FGE)

Plasmid

#16132

Purpose

Bacterial expression of Mycobacterium tuberculosis FGE

Depositor

Carolyn Bertozzi

Article

Carrico et al Nat Chem Biol. 2007 Jun . 3(6):321-2.

Insert

Formylglycine-generating enzyme

Expression

Bacterial

Available Since

Nov. 14, 2007

Availability

Academic Institutions and Nonprofits only

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