184,493 results

1. pTS106 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-GFP

   Plasmid

   #199359

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   3C-SUMOEu-EGFP

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJ1996_v2

   Plasmid

   #140664

   Purpose

   dCas9 & Csy4

   Depositor

   Yolanda Schaerli

   Article

   Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

   Insert

   dCas9 & Csy4
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRS416-yZ3EV-Z3pr-yEGFP (RB3579)

   Plasmid

   #69100

   Purpose

   URA+ yeast shuttle vector containing yZ3EV TF cassette and a Z3pr driving expression of yEGFP

   Depositor

   David Botstein

   Inserts

   yZ3EV cassette
   Z3 promoter
   yEGFP
   

   Expression

   Yeast

   Available Since

   Sept. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Ai139 (TIT2L-GFP-ICL-TPT) targeting vector

   Plasmid

   #114426

   Purpose

   Target a Cre-dependent GFP and a tdTomato-P2A-tTA2 expression cassette into the mouse TIGRE locus

   Depositor

   Hongkui Zeng

   Article

   Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

   Insert

   GFP, tdTomato, tTA2,
   

   Use

   Cre/Lox and Mouse Targeting

   Promoter

   TRE2, CAG

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLX302 EGFP-V5

   Plasmid

   #141348

   Purpose

   Expresses V5 tagged GFP

   Depositor

   Kevin Janes

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   V5 tag

   Expression

   Mammalian

   Available Since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. DL015:CMV::iPAK4

   Plasmid

   #177880

   Purpose

   ibox-PAK4cat (iPAK4) fusion with CMV promoter, cloned into a lentivirus backbone

   Depositor

   Adam Cohen

   Article

   Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   iPAK4
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCVL.SFFV.d14GFP.EF1a.HA.NLS.Sce(opt).T2A.TagBFP

   Plasmid

   #32627

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   Donor + Sce BFP
   

   Use

   Lentiviral

   Available Since

   Nov. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDAS12222_U6-pegRNA-BFP

   Plasmid

   #177181

   Purpose

   pegRNA cloning plasmid with BFP marker

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BirA-Myc_N_PAX6

   Plasmid

   #167721

   Purpose

   Expresses PAX6 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   PAX6 (PAX6 Human)
   

   Tags

   Myc-BirA

   Expression

   Mammalian

   Mutation

   no mutations

   Available Since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.2/NS1

    Plasmid

    #120824

    Purpose

    Ectopic expression of ZIKV protein

    Depositor

    Jan Rehwinkel

    Article

    Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

    Insert

    NS1 ZIKV
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GFP-LiMETER-v2

    Plasmid

    #113934

    Purpose

    Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP

    Depositor

    Yubin Zhou

    Article

    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

    Insert

    LOV2 and Stim1 polybasic domain (PB)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Frt-hspB5

    Plasmid

    #63096

    Purpose

    Expression of HSPB5 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb5 (CRYAB Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. DL016:CMV::eGFP-iPAK4

    Plasmid

    #177881

    Purpose

    eGFP-IPAK4 fusion with CMV promoter, cloned into a lentivirus backbone

    Depositor

    Adam Cohen

    Article

    Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    iPAK4
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLminP_Luc2P_RE50

    Plasmid

    #90389

    Purpose

    PPARg/RARa - PPARg gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    ACO1

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDule-3-nitroTyrosine (5B)

    Plasmid

    #85498

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32H H70C D158S I159A L162R

    Promoter

    lpp (constitutive)

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST-CMV mCherry-GFP-LC3B G120A

    Plasmid

    #123235

    Purpose

    Expresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Mutation

    Glycine 120 to Alanine

    Promoter

    CMV

    Available Since

    Aug. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBAD-pHlorina

    Plasmid

    #182763

    Purpose

    pH-sensitive RFP pHlorina for detection of efferocytosis

    Depositor

    Robert Campbell

    Article

    Raymond et al Science. 2022 Mar 11;375(6585):1182-1187

    Insert

    pHlorina
    

    Expression

    Bacterial

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIRES-hrGFP II-TET1

    Plasmid

    #83568

    Purpose

    Expresses TET1 in mammalian cells

    Depositor

    Jean-Pierre Issa

    Article

    Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

    Insert

    Tet Methylcytosine Dioxygenase 1 (TET1 Human)
    

    Tags

    3X FLAG tag and hr GFP II

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV5-AML1B

    Plasmid

    #12426

    Purpose

    pCMV5-AML1B is the original plasmid used in Meyers et al 1995. However, please note that the insert sequence has now been identified as AML1C.

    Depositor

    Scott Hiebert

    Article

    Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

    Insert

    AML1B (RUNX1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV5-AML1B

    Plasmid

    #12426

    Purpose

    pCMV5-AML1B is the original plasmid used in Meyers et al 1995. However, please note that the insert sequence has now been identified as AML1C.

    Depositor

    Scott Hiebert

    Article

    Meyers et al Mol Cell Biol. 1995 Apr . 15(4):1974-82.

    Insert

    AML1B (RUNX1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge