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pCE-BiFC-VC155

Plasmid

#22020

Depositor

Chang-Deng Hu

Article

Shyu et al Nat Protoc. 2008 . 3(4):588-96.

Insert

VC155

Tags

HA

Expression

Worm

Mutation

Changed Alanine 206 to Lysine

Available Since

Nov. 2, 2009

Availability

Academic Institutions and Nonprofits only

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Str-Ii_SBP-EGFP-Golgin84

Plasmid

#65303

Purpose

synchronize trafficking of Golgin-84 from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Golgin-84 (GOLGA5 Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pSFFV_sfCherry2_TagBFP

Plasmid

#83031

Purpose

Expresses full length sfCherry2 and TagBFP fusion in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry+full length_TagBFP

Use

Lentiviral

Expression

Mammalian

Mutation

3 mutations in sfCherry full length: E118Q, T128I…

Promoter

SFFV

Available Since

Jan. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pTwist-CMV-HDGFL2_Cryptic_TwinstrepTEV_6xHis

Plasmid

#232344

Purpose

Expresses human HDGFL2 protein with TDP-43 regulated cryptic exon, along with an N-terminal twinstrep tag + TEV protease site and C-terminal His-tag

Depositor

Philip Wong

Article

Irwin et al Nat Med. 2024 Feb;30(2):382-393. doi: 10

Insert

HDGF Like 2 (HDGFL2 Human)

Tags

GGGS linker, 6xHis and Twin-strep, TEV protease s…

Expression

Mammalian

Mutation

TDP-43 regulated cryptic exon

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pTET2-RlucCP

Plasmid

#198352

Purpose

Part of Firefly reporter system, encodes Renilla luciferase

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

Renilla Luciferase

Use

Luciferase

Expression

Mammalian

Promoter

pTET2

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR223-BGLF5

Plasmid

#195813

Purpose

Gateway Entry Clone of Epstein-Barr virus BGLF5

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BGLF5 (BGLF5 Epstein-Barr virus)

Use

Gateway Cloning

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-His6

Plasmid

#182386

Purpose

Bacterial expression of Vesicle Nucleating peptide

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-mCherry-raptor

Plasmid

#73386

Purpose

Expresses raptor tagged with mCherry and HA

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

Regulatory Associated Protein Of MTOR, Complex 1 (RPTOR Human)

Tags

HA and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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KRAB-dSpCas9

Plasmid

#205248

Purpose

In vitro transcription plasmid for production of KOX1 KRAB-dSpCas9 mRNA for transcriptional repression (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #85449.

Depositor

Rasmus Bak

Article

Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

Insert

KRAB-dSpCas9

Use

CRISPR

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCE-BiFC-VN173

Plasmid

#22019

Depositor

Chang-Deng Hu

Article

Shyu et al Nat Protoc. 2008 . 3(4):588-96.

Insert

VN173

Tags

Myc

Expression

Worm

Mutation

N/A

Available Since

Oct. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaA

Plasmid

#50386

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PanK (pantothenate kinase), induced with IPTG. Contain 6xHis-tag for nickel affinity purification.

Depositor

Erick Strauss

Article

Brand et al J Biol Chem. 2005 May 27;280(21):20185-8

Insert

PanK

Tags

6XHis-tag

Expression

Bacterial

Promoter

T7

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only

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pCSC-NES-RFP

Plasmid

#221812

Purpose

Lentiviral vector expressing red fluorescent protein (RFP) fused with nuclear export sequence (NES)

Depositor

Baojin Ding

Article

Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

Insert

NES-mCherry

Use

Lentiviral

Tags

NES

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pACYC-prk:nptII

Plasmid

#239580

Purpose

Selection plasmid for Rubisco directed evolution

Depositor

Matthew D. Shoulders

Article

McDonald et al Proc Natl Acad Sci U S A. 2025 Jul 8;122

Insert

PRK-NPTII

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_CKAR3

Plasmid

#238411

Purpose

Mammalian expression of CKAR3 under a CMV promotor.

Depositor

Haining Zhong

Article

Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

Insert

CKAR3 (PKC sensor)

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209724

Purpose

Expresses cytosolic low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(cyto).iATPSnFR2.A95A.A119L.HaloTag

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CMVs.TurboRFP.WPRE.RBG

Plasmid

#105548

Purpose

AAV expression of TurboRFP from CMV promoter

Depositor

James M. Wilson

Has Service

AAV1 and AAV8

Insert

TurboRFP

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pfMDH_WT

Plasmid

#67721

Purpose

Expresses WT mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

Depositor

Justin Siegel

Article

Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

Insert

Mannitol 2-Dehydrogenase from Pseudomonas fluorescens

Tags

6x-His tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
Cultivarium MACKEREL Library

Pooled Library

#208054

Purpose

pGL0_151 [RS421] is a library of broad host range prokaryotic promoters-RBS regulatory sequences formatted as a golden gate assembly Level 0 part.

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Use

Synthetic Biology

Available Since

March 21, 2024

Availability

Academic Institutions and Nonprofits only
pSF280

Plasmid

#167675

Purpose

Intermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCFD1-dU6:1gRNA

Plasmid

#49408

Purpose

expression of gRNA under control of the Drosophila U6:1 promoter

Depositor

Simon Bullock

Article

Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

Insert

dU6-1:gRNA

Use

CRISPR

Expression

Insect

Promoter

dU6-1

Available Since

Jan. 10, 2014

Availability

Academic Institutions and Nonprofits only

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