235,241 results
-
Plasmid#64697PurposeH6-Ulp1 from pHYRS52 into pET-28b(+)DepositorInsertULP1 (ULP1 Budding Yeast, Saccharomyces cerevisiae)
Tags6xHisExpressionBacterialMutationUlp1(403-621)PromoterT7Available sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MCS-13X Linker-BioID2-HA
Plasmid#80899PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.DepositorTypeEmpty backboneTagsHA and humanized A. aeolicus BioID2 with R40GExpressionMammalianPromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MCS-13X Linker-BioID2-HA
Plasmid#80899PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.DepositorTypeEmpty backboneTagsHA and humanized A. aeolicus BioID2 with R40GExpressionMammalianPromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMXs-Klf4
Plasmid#13370PurposeRetroviral expression of mouse Klf4DepositorAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMXs-Klf4
Plasmid#13370PurposeRetroviral expression of mouse Klf4DepositorAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEF-GFP
Plasmid#11154PurposeMammalian expression vector for expression of GFP (EF1a promoter)DepositorAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEF-GFP
Plasmid#11154PurposeMammalian expression vector for expression of GFP (EF1a promoter)DepositorAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA4 myc PGC-1 alpha
Plasmid#10974PurposeMammalian expression of PGC-1a with myc and his tagsDepositorAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA4 myc PGC-1 alpha
Plasmid#10974PurposeMammalian expression of PGC-1a with myc and his tagsDepositorAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MLM3636
Plasmid#43860Purposeguide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoterDepositorInsertNone
UseCRISPRExpressionMammalianPromoterU6Available sinceMarch 8, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MLM3636
Plasmid#43860Purposeguide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoterDepositorInsertNone
UseCRISPRExpressionMammalianPromoterU6Available sinceMarch 8, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-EGFP (AAV9)
Viral prep#50465-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEGFPAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPSU1
Plasmid#89439PurposeHigh copy number plasmid 1 to prepare Penn State DNA molecular weight laddersDepositorInsertsExpressionBacterialAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pgRNA-bacteria
Plasmid#44251PurposeExpression of customizable guide RNA (gRNA) for bacterial gene knockdownDepositorInsertguide RNA (bacteria)
UseCRISPRExpressionBacterialPromoterBBa_J23119 (SpeI)Available sinceApril 5, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pgRNA-bacteria
Plasmid#44251PurposeExpression of customizable guide RNA (gRNA) for bacterial gene knockdownDepositorInsertguide RNA (bacteria)
UseCRISPRExpressionBacterialPromoterBBa_J23119 (SpeI)Available sinceApril 5, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEVOL-pAzF
Plasmid#31186PurposetRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAGDepositorInsertM.j. p-azidohenylalanine RS (2 copies +tRNA)
ExpressionBacterialMutationY32T, E107N, D158P, I159L, L162Q, D286RAvailable sinceDec. 9, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEVOL-pAzF
Plasmid#31186PurposetRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAGDepositorInsertM.j. p-azidohenylalanine RS (2 copies +tRNA)
ExpressionBacterialMutationY32T, E107N, D158P, I159L, L162Q, D286RAvailable sinceDec. 9, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
3X ERE TATA luc
Plasmid#11354PurposeLuciferase reporter containing three copies of vitellogenin Estrogen Response ElementDepositorInsertERE
UseLuciferaseExpressionBacterialAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
3X ERE TATA luc
Plasmid#11354PurposeLuciferase reporter containing three copies of vitellogenin Estrogen Response ElementDepositorInsertERE
UseLuciferaseExpressionBacterialAvailable sinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-PEmax-P2A-hMLH1dn
Plasmid#174828PurposeMammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)DepositorInsertPEmax-P2A-hMLH1dn
TagsSV40 bpNLS and c-Myc NLSExpressionMammalianMutationDetailed in manuscript; deletion of residues 754-…PromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by