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pDESTmycIGF2BP3

Plasmid

#19879

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3 Human)

Tags

MYC

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pDESTmycIGF2BP3

Plasmid

#19879

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3 Human)

Tags

MYC

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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Mouse sgRNA library Brie in lentiGuide-Puro

Pooled Library

#73633

Purpose

Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Has Service

Lentiviral Prep

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only
Mouse sgRNA library Brie in lentiGuide-Puro

Pooled Library

#73633

Purpose

Mouse sgRNA library in backbone lentiGuide-Puro containing 78,637 unique sgRNAs targeting 19,674 genes along with 1000 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Has Service

Lentiviral Prep

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only
LentiSAMPHv2

Plasmid

#167934

Purpose

All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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LentiSAMPHv2

Plasmid

#167934

Purpose

All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 LIC cloning vector (6A)

Plasmid

#30124

Depositor

Scott Gradia

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3 LIC cloning vector (6A)

Plasmid

#30124

Depositor

Scott Gradia

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-human-Caspase-4

Plasmid

#183356

Purpose

Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-4 (CASP4 Human)

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-GFP-human-Caspase-4

Plasmid

#183356

Purpose

Expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

Insert

Caspase-4 (CASP4 Human)

Use

Retroviral

Tags

Myc

Promoter

MESV

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK793

Plasmid

#8827

Depositor

David Waugh

Article

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

TEV protease, S219V mutant

Tags

His, MBP (with TEV), and polyarginine

Expression

Bacterial

Mutation

S219V mutation (improves stability)

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pRK793

Plasmid

#8827

Depositor

David Waugh

Article

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

TEV protease, S219V mutant

Tags

His, MBP (with TEV), and polyarginine

Expression

Bacterial

Mutation

S219V mutation (improves stability)

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pDG1-PHP.eB

Plasmid

#220799

Purpose

AAV helper plasmid expressing E4, E2A, VA, AAV2 rep and PHP.eB cap genes

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

E4, E2A, VA, AAV2 rep and PHP.eB cap genes

Use

AAV; Aav helper vector

Expression

Mammalian

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDG1-PHP.eB

Plasmid

#220799

Purpose

AAV helper plasmid expressing E4, E2A, VA, AAV2 rep and PHP.eB cap genes

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

E4, E2A, VA, AAV2 rep and PHP.eB cap genes

Use

AAV; Aav helper vector

Expression

Mammalian

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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TFORF3054

Plasmid

#144530

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SALL4 (SALL4 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3054

Plasmid

#144530

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SALL4 (SALL4 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-gDA3m

Plasmid

#208698

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Has Service

AAV1

Insert

GPCR activation based dopamine (DA) sensor GRAB_gDA3m

Use

AAV

Promoter

hSyn

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-gDA3m

Plasmid

#208698

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3m in neurons

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Has Service

AAV1

Insert

GPCR activation based dopamine (DA) sensor GRAB_gDA3m

Use

AAV

Promoter

hSyn

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pSAM_Bt

Plasmid

#112497

Purpose

Transposon mutagenesis vector for Bacteroides thetaiotaomicron. Compatible with Insertion Sequencing (INSeq).

Depositor

Andrew Goodman, Jeffrey Gordon

Article

Goodman et al Cell Host Microbe. 2009 Sep 17;6(3):279-

Inserts

INSeq-adapted mariner transposon
Himar1C9

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pSAM_Bt

Plasmid

#112497

Purpose

Transposon mutagenesis vector for Bacteroides thetaiotaomicron. Compatible with Insertion Sequencing (INSeq).

Depositor

Andrew Goodman, Jeffrey Gordon

Article

Goodman et al Cell Host Microbe. 2009 Sep 17;6(3):279-

Inserts

INSeq-adapted mariner transposon
Himar1C9

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge