Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

236,784 results

Showing: 421 - 440 of 236784 results

  1. Golden Gate TALEN and TAL effector kit 2.0

    Plasmid kit
    #1000000024
    Purpose
    Assemble TALEN constructs with custom repeat arrays between 12 and 31 repeats via Golden Gate cloning for expression in multiple systems.
    Depositor
    Adam Bogdanove, Daniel Voytas
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector type
    Mammalian Expression, Plant Expression, Yeast Expression
    Editing type
    TALEN
    Cloning type
    Golden Gate
    Available since
    Jan. 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  2. Human sgRNA library Brunello in lentiCRISPRv2 (Lentiviral Prep)

    Viral prep
    #73179-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiCRISPRv2 (#73179). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2 plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. This backbone contains SpCas9 and unique gRNAs, and can be used to make edits across 19,114 genes in the human genome. In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2.
    Depositor
    John Doench, David Root
    Publication
    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103
    Available since
    June 14, 2017
    Availability
    Academic Institutions and Nonprofits only

  5. MCS-13X Linker-BioID2-HA

    Plasmid
    #80899
    Purpose
    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.
    Depositor
    Kyle Roux
    Publication
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Type
    Empty backbone
    Tags
    HA and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80899-plasmid-map-sequence-id-181185

    Enlarge

  6. MCS-13X Linker-BioID2-HA

    Plasmid
    #80899
    Purpose
    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.
    Depositor
    Kyle Roux
    Publication
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Type
    Empty backbone
    Tags
    HA and humanized A. aeolicus BioID2 with R40G
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80899-plasmid-map-sequence-id-181185

    Enlarge

  11. pAAV-hSyn-EGFP (AAV9)

    Viral prep
    #50465-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP
    Available since
    May 28, 2019
    Availability
    Academic Institutions and Nonprofits only

  12. pAAV-hSyn-DIO-mCherry (AAV9)

    Viral prep
    #50459-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    April 25, 2019
    Availability
    Academic Institutions and Nonprofits only

  14. pEVOL-pAzF

    Plasmid
    #31186
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG
    Depositor
    Peter Schultz
    Publication
    Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7
    Insert
    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    Expression
    Bacterial
    Mutation
    Y32T, E107N, D158P, I159L, L162Q, D286R
    Available since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    31186-plasmid-map-sequence-id-334781

    Enlarge

  15. pEVOL-pAzF

    Plasmid
    #31186
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG
    Depositor
    Peter Schultz
    Publication
    Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7
    Insert
    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    Expression
    Bacterial
    Mutation
    Y32T, E107N, D158P, I159L, L162Q, D286R
    Available since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    31186-plasmid-map-sequence-id-334781

    Enlarge

  16. MLM3636

    Plasmid
    #43860
    Purpose
    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter
    Depositor
    Keith Joung
    Insert
    None
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    March 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    43860-plasmid-map-sequence-id-194368

    Enlarge

  17. MLM3636

    Plasmid
    #43860
    Purpose
    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence, uses U6 promoter
    Depositor
    Keith Joung
    Insert
    None
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    March 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    43860-plasmid-map-sequence-id-194368

    Enlarge

  20. pPSU1

    Plasmid
    #89439
    Purpose
    High copy number plasmid 1 to prepare Penn State DNA molecular weight ladders
    Depositor
    Song Tan
    Publication
    Henrici et al Sci Rep. 2017 May 26;7(1):2438. doi: 10.
    Inserts
    500 bp EcoRV fragment
    1000 bp EcoRV fragment
    1500 bp EcoRV fragment
    2000 bp EcoRV fragment
    500 bp PstI fragment
    700 bp PstI fragment
    800 bp PstI fragment (EHMT2 Human)
    900 bp PstI fragment
    1000 bp PstI fragment (BMI1 Human)
    2000 bp PstI fragment
    Expression
    Bacterial
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    89439-plasmid-map-sequence-id-190157

    Enlarge

Showing: 421 - 440 of 236784 results