Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

254,009 results

Showing: 421 - 440 of 254009 results

  3. pcDNA3-mRuby2

    Plasmid
    #40260
    Purpose
    RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap
    Depositor
    Michael Lin
    Publication
    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mRuby2
    Tags
    His6 and Xpress
    Expression
    Mammalian
    Promoter
    CMV IE
    Available since
    Sept. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    40260-plasmid-map-sequence-id-243381

    Enlarge

  4. pcDNA3-mRuby2

    Plasmid
    #40260
    Purpose
    RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap
    Depositor
    Michael Lin
    Publication
    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mRuby2
    Tags
    His6 and Xpress
    Expression
    Mammalian
    Promoter
    CMV IE
    Available since
    Sept. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    40260-plasmid-map-sequence-id-243381

    Enlarge

  11. pAAV-hSyn-DIO-mCherry (AAV2)

    Viral prep
    #50459-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    Nov. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  12. Human sgRNA library Brunello in lentiCRISPRv2 (Lentiviral Prep)

    Viral prep
    #73179-LV
    Purpose
    Ready-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiCRISPRv2 (#73179). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2 plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. This backbone contains SpCas9 and unique gRNAs, and can be used to make edits across 19,114 genes in the human genome. In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2.
    Depositor
    John Doench, David Root
    Publication
    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103
    Available since
    June 14, 2017
    Availability
    Academic Institutions and Nonprofits only

  13. pAAV-hSyn-DIO-mCherry (AAV9)

    Viral prep
    #50459-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-DIO-mCherry (#50459). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-mCherry plasmid DNA. hSyn-driven, Cre-dependent mCherry-expression control. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry (Cre-dependent)
    Available since
    April 25, 2019
    Availability
    Academic Institutions and Nonprofits only

  16. Golden Gate TALEN and TAL effector kit 2.0

    Plasmid kit
    #1000000024
    Purpose
    Assemble TALEN constructs with custom repeat arrays between 12 and 31 repeats via Golden Gate cloning for expression in multiple systems.
    Depositor
    Adam Bogdanove, Daniel Voytas
    Application
    Cloning and Synthetic Biology, Genome Editing
    Vector type
    Mammalian Expression, Plant Expression, Yeast Expression
    Editing type
    TALEN
    Cloning type
    Golden Gate
    Available since
    Jan. 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  17. pAAV-hSyn-EGFP (AAV9)

    Viral prep
    #50465-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-EGFP (#50465). In addition to the viral particles, you will also receive purified pAAV-hSyn-EGFP plasmid DNA. hSyn-driven EGFP expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    EGFP
    Available since
    May 28, 2019
    Availability
    Academic Institutions and Nonprofits only

  20. pEVOL-pAzF

    Plasmid
    #31186
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG
    Depositor
    Peter Schultz
    Publication
    Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7
    Insert
    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    Expression
    Bacterial
    Mutation
    Y32T, E107N, D158P, I159L, L162Q, D286R
    Available since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    31186-plasmid-map-sequence-id-334781

    Enlarge

Showing: 421 - 440 of 254009 results