168,838 results

1. pAAV-CAG-miniGFP2-P2A-FusionRed

   Plasmid

   #188734

   Purpose

   Express miniGFP2 and FusionRed in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

   Insert

   miniGFP2
   

   Use

   AAV

   Tags

   FusionRed

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Halo-Rab6A

   Plasmid

   #190171

   Purpose

   Expresses HaloTag-Rab6A in mammalian cells.

   Depositor

   Anna Akhmanova

   Article

   Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

   Insert

   HaloTag-Rab6A
   

   Tags

   HaloTag

   Expression

   Mammalian

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHSS6hsILMi20

   Plasmid

   #102536

   Purpose

   Helper plasmid expressing Minos transposase mRNA (Drosophila hsp70 promoter)

   Depositor

   Michalis Averof, Charalambos Savakis

   Article

   Klinakis et al Insect Mol Biol. 2000 Jun;9(3):269-75.

   Insert

   Minos transposase
   

   Available Since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDY0216 PASTEv1 PE2-P2A-Bxb1

   Plasmid

   #179104

   Purpose

   PASTEv1 PE2-P2A-Bxb1

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   pCMV-PE2-P2A-Bxb1
   

   Expression

   Mammalian

   Available Since

   Feb. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Fucci(CA) hCdt1-iRFP hGeminin-TagBFP2

   Plasmid

   #190181

   Purpose

   Fluorescent reporter vector to visualize all of cell cycle phases.

   Depositor

   Toshiro Sato

   Article

   Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

   Inserts

   iRFP
   hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
   TagBFP2
   hGeminin(1/110)
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. LEGO-AP1x6-GM55

   Plasmid

   #217415

   Purpose

   Luciferase expression vector with 6 AP-1 sites and the minimal GM-CSF promoter. Alias: LEGO-GM55-AP-1x6

   Depositor

   Peter Cockerill

   Insert

   Luciferase, GFP
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   6 AP-1 sites, Human -55 to +28 minimal CSF2 promo…

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-mArg1 promoter/enhancer -31/-3810

   Plasmid

   #34571

   Depositor

   Peter Murray

   Article

   Pauleau et al J Immunol. 2004 Jun 15;172(12):7565-73.

   Insert

   Arg1 promoter/enhancer -31/-3810 (Arg1 Mouse)
   

   Use

   Luciferase

   Tags

   Firefly Luciferase

   Available Since

   Jan. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBAD42-BtuCEDFB

   Plasmid

   #237541

   Purpose

   plasmid encodes BtuC, BtuD, BtuE, BtuF, BtuB for enhanced solubilization of cobalamin proteins

   Depositor

   Squire Booker

   Article

   Lanz et al Biochemistry. 2018 Mar 6;57(9):1475-1490

   Inserts

   btuB
   btuF
   btuCED
   

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   Sept. 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-UCOE-EF1a-Zim3-dCas9-BFP

   Plasmid

   #188775

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9-mTagBFP2
   

   Use

   Lentiviral

   Tags

   HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

   Promoter

   EF1a

   Available Since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Mouse lipid metabolism knockout library

    Pooled Library

    #206248

    Purpose

    The lipid metabolism library contains guide RNAs against 295 genes targeting mouse genes encoding enzymes that directly impact lipid metabolism.

    Depositor

    Kivanc Birsoy

    Article

    Soula et al Nature. 2024 Aug 7. doi: 10.1038/s41586-

    Use

    CRISPR and Lentiviral

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

11. STARR-seq luciferase validation vector_ORI_empty

    Plasmid

    #99297

    Purpose

    Luciferase validation vector; empty vector without insert to determine baseline

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Luciferase; Starr-seq luciferase validation vector

    Expression

    Mammalian

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. CROPseq-iT7

    Plasmid

    #211699

    Purpose

    NIS-Seq-compatible lentiviral vector for expressing sgRNAs

    Depositor

    Jonathan Schmid-Burgk

    Article

    Fandrey et al Nat Biotechnol. 2024 Dec 19. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSN837

    Plasmid

    #184825

    Purpose

    Expresses human KIF5A in mammalian cells

    Depositor

    Kyoko Chiba

    Article

    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

    Insert

    KIF5A (KIF5A Human)
    

    Tags

    mScarlet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKS1

    Plasmid

    #84079

    Purpose

    Expresses the non-toxic fragment C of tetanus toxin

    Depositor

    Neil Fairweather

    Article

    Sinha et al Mol Microbiol. 2000 Sep;37(5):1041-51.

    Insert

    Tetanus neurotoxin fragment C
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-hSynapsin-Cre-WPRE

    Plasmid

    #86641

    Purpose

    Expresses Cre in mammalian cells

    Depositor

    Fan Wang

    Article

    Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

    Insert

    Cre
    

    Use

    Cre/Lox, Lentiviral, Mouse Targeting, and Synthet…

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDF-NtXNtS1R1R2B2

    Plasmid

    #160882

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and raf2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcS-S1, rbcX, raf1, raf2, bsd2
    

    Expression

    Bacterial

    Promoter

    rbcS-S1: T7, chaperones: T7

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid

    #175570

    Purpose

    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

    Depositor

    Joseph Brzezinski

    Type

    Empty backbone

    Use

    CRISPR

    Mutation

    XbaI site added upstream of U6 promotor. G to C …

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-C1-Ataxin3Q28

    Plasmid

    #22122

    Depositor

    Henry Paulson

    Article

    Chai et al Proc Natl Acad Sci U S A. 2002 Jul 9. 99

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Oct. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. mTagRFP-T-Lifeact-7

    Plasmid

    #54586

    Purpose

    Localization: Actin, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Insert

    Lifeact-mTagRFP-T
    

    Expression

    Mammalian

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pETNb-cALFA

    Plasmid

    #159989

    Purpose

    For the protein expression with ALFA tag at C-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Bacterial

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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