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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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175,412 results

Showing: 421 - 440 of 175412 results

  3. Reb_1 aka PAS619

    Plasmid
    #71224
    Purpose
    Expresses wild type R bodies from C. taeniospiralis in pETM11 backbone
    Depositor
    Pamela Silver
    Article
    Polka et al ACS Synth Biol. 2016 Feb 3.
    Insert
    Reb locus
    Tags
    none
    Expression
    Bacterial
    Mutation
    RebA G53A
    Promoter
    T7
    Available Since
    April 7, 2016
    Availability
    Academic Institutions and Nonprofits only

  6. eSpCas9(1.1)-Puro

    Plasmid
    #117686
    Purpose
    The plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.
    Depositor
    Luc Leyns
    Article
    Liu et al Biochem Biophys Res Commun. 2019 Feb 14.
    Insert
    eSpCas9(1.1)
    Use
    CRISPR
    Tags
    PuroR (R166H)
    Expression
    Mammalian
    Mutation
    K848A, K1003A, R1060A
    Promoter
    U6
    Available Since
    Feb. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    117686-plasmid-map-sequence-id-236350

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  10. AICSDP-80:UBTF-HaloTag

    Plasmid
    #133963
    Purpose
    Homology arms and HaloTag-linker sequence for N-terminus tagging of human UBTF
    Depositor
    Allen Institute for Cell Science
    Insert
    UBTF Homology Arms with HaloTag-linker (UBTF Human)
    Use
    CRISPR
    Tags
    HaloTag-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Nov. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    133963-plasmid-map-sequence-id-264347

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  11. TRE3GV-somBiPOLES-mKate2

    Plasmid
    #192579
    Purpose
    AAV-vector for bidirectional optogenetic manipulation of tTA-positive neurons
    Depositor
    Simon Wiegert
    Insert
    somBiPOLES
    Use
    AAV
    Tags
    soma-targeting motif from Kv2.1 channel
    Expression
    Mammalian
    Promoter
    TRE3GV
    Available Since
    Dec. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    192579-plasmid-map-sequence-id-379367

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  15. Dendra2-CathespinB-6

    Plasmid
    #57703
    Purpose
    Localization: Lysosomes, Excitation: 490 / 553, Emission: 507 / 573
    Depositor
    Michael Davidson
    Insert
    CathespinB
    Tags
    Dendra2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 16, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. TFORF3350

    Plasmid
    #144826
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TBX15 (TBX15 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144826-plasmid-map-sequence-id-404424

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  18. pcDNA3-Flag-Tet2 CD Mut

    Plasmid
    #72220
    Purpose
    Express flag-tagged mouse Tet2 catalytic domain - catalytic dead mutant.
    Depositor
    Yi Zhang
    Article
    Ito et al Science. 2011 Sep 2;333(6047):1300-3. do
    Insert
    Ten-Eleven Translocation 2 (Tet2 Mouse)
    Tags
    flag
    Expression
    Mammalian
    Mutation
    The plasmid expresses TET2 catalytic domain (cata…
    Promoter
    CMV
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only

  19. pB-TR-hSOD1

    Plasmid
    #232478
    Purpose
    Tetracycline inducible PiggyBac vector expressing human SOD1gene including flag-tag (DYKDDDDK) on the 3' end
    Depositor
    Lukas Trantirek
    Article
    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1
    Insert
    Homo sapiens superoxide dismutase 1 (SOD1 Human)
    Use
    Transposon system
    Tags
    DYKDDDDK (FLAG-tag)
    Expression
    Mammalian
    Promoter
    CMV-TetO
    Available Since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    232478-plasmid-map-sequence-id-471680

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Showing: 421 - 440 of 175412 results