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pAAV-hSyn-GRAB_g5-HT3.0

Plasmid

#208717

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Has Service

AAV8

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0

Use

AAV

Promoter

hSyn

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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hGli1 flag3x

Plasmid

#84922

Purpose

mammalian expression of Gli1

Depositor

Martin Fernandez-Zapico

Insert

Gli1 (GLI1 Human)

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pAGT9569

Plasmid

#219431

Purpose

Transcriptional unit (MoClo-position 2): pAtRPS5a_PapE-4LF2-N-SpCas9i-N_tNOS

Depositor

Tom Schreiber

Article

Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

Insert

LB_AtRPS5a:PapE-4xLF2-NLS-SpCas9i-NLS:tNOS_RB (Position 2)

Use

CRISPR and Synthetic Biology; Moclo compatible le…

Tags

PapE-4LF2

Expression

Plant

Available Since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-pCMV-dSaCas9-VP64-pU6-sgRNA

Plasmid

#158990

Purpose

Vector G encodes pAAV-pCMV-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in mammalian cells

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP64

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pCMV

Available Since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pMRX-hSTING (N154S)-EGFP

Plasmid

#214150

Purpose

Retroviral transduction of STING

Depositor

Nan Yan

Article

Chu et al Nature. 2021 Aug;596(7873):570-575. doi:

Insert

STING (STING1 Human)

Use

Retroviral

Tags

EGFP

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-puro /BRAF-V600E

Plasmid

#131724

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

Insert

BRAF (BRAF Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

V600E

Promoter

CMV

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pUbiGUSPlus

Plasmid

#64402

Purpose

Ubiquitin promoter driving synthetic optimised GUSPlus gene. Can be used as a reporter of promoter activity in plants by replacing Ubi promoter with sequence of interest.

Depositor

Claudia Vickers

Article

Vickers et al Plant Cell Rep. 2003 Sep;22(2):135-40. E

Insert

ubiquitin (Ubi-1) promoter

Tags

GUSPlus reporter

Expression

Plant

Promoter

Ubi-1

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pUbiGUSPlus

Plasmid

#64402

Purpose

Ubiquitin promoter driving synthetic optimised GUSPlus gene. Can be used as a reporter of promoter activity in plants by replacing Ubi promoter with sequence of interest.

Depositor

Claudia Vickers

Article

Vickers et al Plant Cell Rep. 2003 Sep;22(2):135-40. E

Insert

ubiquitin (Ubi-1) promoter

Tags

GUSPlus reporter

Expression

Plant

Promoter

Ubi-1

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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AP1muA-Halo-ALFA-polyA-G418 HR

Plasmid

#229681

Purpose

Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.

Depositor

Francesca Bottanelli

Article

Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

Insert

genomic sequence AP1M1 locus (AP1M1 Human)

Use

CRISPR

Tags

Halo, ALFA

Available Since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1 mCherry-NLS

Plasmid

#58476

Purpose

Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

Depositor

Dyche Mullins

Article

Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

Insert

3X NLS with 3X stop codons

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1 mCherry-NLS

Plasmid

#58476

Purpose

Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

Depositor

Dyche Mullins

Article

Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

Insert

3X NLS with 3X stop codons

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt5A

Plasmid

#35911

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5A (WNT5A Human)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt5A

Plasmid

#35911

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5A (WNT5A Human)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pNP3396 [pNP2922 - ISCro4 DBL1 (WT) + ISCro4 Recombinase (WT)]

Plasmid

#247259

Purpose

Express ISCro4 recombinase and bridge RNA

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Insert

pNP2922 - ISCro4 bRNA112-179 (DBL1)

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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NanoBRET Assay Vector, CRAF(FL)-HaloTag

Plasmid

#236874

Purpose

Express CRAF(FL)-HaloTag(R) in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

CRAF (RAF1 Human)

Tags

HaloTag (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV.CAG.(cyto).cpSFGFP.HaloTag

Plasmid

#209654

Purpose

Non-responsive control

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

cpSFGFP.HaloTag

Use

AAV

Promoter

CAG

Available Since

Nov. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-NBid-PhoCl2c-CBid

Plasmid

#164051

Purpose

Optogenetic manipulation of cell apoptosis using PhoCl2c

Depositor

Robert Campbell

Article

Lu et al Chem Sci. 2021 May 31;12(28):9658-9672.

Insert

NBid-PhoCl2c-CBid

Tags

kozak sequence

Expression

Mammalian

Promoter

CMV promotor

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAM5505

Plasmid

#132664

Purpose

pRL623-based helper plasmid for conjugations, with RSF1010 mobA gene

Depositor

Susan Golden

Article

Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

Insert

MobA

Expression

Bacterial

Mutation

please see depositor comments below

Promoter

p1,p3

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-A2UCOE-EF1a-mCR1-F2A-neo-AAVS1

Plasmid

#205447

Purpose

human AAVS1-targeting plasmid for expression of mouse CR1

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCR1

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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mNeon-Vamp2

Plasmid

#186619

Purpose

Encodes Vamp 2 fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Vesicle Associated Membrane Protein 2 (VAMP2 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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