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Plasmid#14685DepositorInsertbeta-arrestin 2 (Arrb2 Rat)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceJune 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
The Expanded EasyClone-MarkerFree toolkit
Plasmid Kit#1000000190PurposeThe Expanded EasyClone-MarkerFree toolkit is a new set of 8 validated chromosomal loci for the stable integration of heterologous DNA into baker’s yeast Saccharomyces cerevisiae.DepositorAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/FLAG/HA-DEST TNRC6B
Plasmid#19884DepositorInserttrinucleotide repeat containing 6B (TNRC6B Human)
UseTagsFLAG/HAExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-SZT2
Plasmid#87035Purposemammalian expression of SZT2DepositorInsertSZT2 (SZT2 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
CD4-MKII-hLOV1-TEVcs(ENLYFQ/M)- Gal4
Plasmid#163026PurposeFLiCRE TF for HEK293T expressionDepositorInsertCD4-MKII-hLOV1-TEVcs-Gal4
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-tdTomato-WPRE
Plasmid#202610PurposeDouble floxed red fluorescent protein tdTomato in AAV production vector expressed under the mammalian promoter (EF1a)DepositorInserttdTomato
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HisYFP-Sp100
Plasmid#53141PurposeE.coli expression of HisYFP tagged human Sp100 fragmentDepositorInsertSp100A fragment (SP100 Human)
UseTagsHis-YFPExpressionBacterialMutationfragment (amino acids 71-480); deletion of L75PromoterT7Available sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-mSin1(1.1)-HA
Plasmid#73388PurposeExpresses mSin-HADepositorInsertmSin1 transcript variant protein isoform 1 (MAPKAP1 Human)
UseTagsHA tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-hSpCas9
Plasmid#162162PurposeExpresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)DepositorInsertshSpCas9
sgRNA
UseCRISPRTagsN/AExpressionInsectMutationPromoterAe. aegypti PUb and Ae. aegypti U6Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsDIS3_Z
Plasmid#148111PurposeMammalian Expression of HsDIS3DepositorInsertHsDIS3 (DIS3 Human)
UseTagsExpressionMammalianMutationL760F compared to the sequence given by isoform 2…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-3xFLAG-hSpCas9
Plasmid#162161PurposeExpresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)DepositorInsertshSpCas9
sgRNA
UseCRISPRTags3xFLAGExpressionInsectMutationPromoterAe. aegypti PUb and Ae. aegypti U6Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEMS2113
Plasmid#49138PurposeAAV plasmid with FEV (Ple67) promoter driving expression of EmGFP. Contains WPRE.DepositorInsertssAAV-Ple67-emGFP-WPRE
UseAAVTagsExpressionMammalianMutationPromoterFEVAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMXS-IRES-Blast mAtg7
Plasmid#173170PurposeRetroviral vector to express sgRNA resistant mouse Atg7DepositorInsertAutophagy Related 7 (Atg7 Mouse)
UseRetroviralTagsExpressionMutationSynonymous mutations at sgRNA sitesPromoterMMLVAvailable sinceFeb. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1
Plasmid#162164PurposeSelectable homology donor template for Aag2 cell AGO1DepositorInsertsRFP
3xFLAG
UseCRISPR and Cre/Lox; Homology donor templateTagsAGO1 homology and loxPExpressionInsectMutationPromoterAe. aegypti PUb and endogenous AGO1Available sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 RagA shRNA #1
Plasmid#30319DepositorInsertRagA shRNA (RRAGA Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
TFORF2923
Plasmid#143674PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNFIA (NFIA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-iCas9
Plasmid#160048Purposeinducible expression of Cas9DepositorInsertCas9
UseTagsExpressionMammalianMutationPromoterTREAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTALEN(Nd154C63) MND
Plasmid#50432PurposepTAL plasmid compatible with the Golden Gate TALEN cloning system, for cloning of TALENs and expression in mammalian cells.DepositorTypeEmpty backboneUseLentiviral and TALENTagsExpressionMammalianMutationPromoterMNDAvailable sinceMay 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 RagA shRNA #2
Plasmid#30320DepositorInsertRagA shRNA (RRAGA Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAMBIA-FLS2prom::FLS2-3xmyc-GFP
Plasmid#86157PurposeFLS2-GFP expresssionDepositorInsertFLS2 (FLS2 Mustard Weed)
UseTags3xmyc and GFPExpressionPlantMutationPromoterAvailable sinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only