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Plasmid#68044PurposeLgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in body wall muscle, for monitoring autophagyDepositorInsertpMyo-3::oxCerulean-oxVenus::Lgg-1
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSB700 Cerulean-Weissman scaffold
Plasmid#79377PurposeCas9 gRNA variant to recruit ms2 proteinsDepositorInsertscaffold
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSB700-mouse-TTN-Cerulean
Plasmid#128325PurposeSP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)DepositorInsertgRNA against mouse TTN for activation
UseTagsExpressionMammalianMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK Hygro DEST H2B-mCerulean3
Plasmid#90234PurposeLentiviral vector to express H2B-mCerulean3 under PGK promoter (With Hygromycin Resistance)DepositorInsertH2B
UseLentiviralTagsmCerulean3ExpressionMammalianMutationPromoterPGKAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
paavCAG-JxON-pre-mGRASP-mCerulean
Plasmid#51903PurposeJxOn-preDepositorInsertpresynaptic mGRASP
UseAAV and Cre/LoxTagsExpressionMutationPromoterCAGAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
PM-FRB-Cerulean-T2A-FKBP-5-ptase
Plasmid#40897DepositorInsertPM-FRB-Cerulean-T2A-FKBP-5-ptase
UseTagsExpressionMutationPromoterAvailable sinceJan. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
raACR-TS-Kv-P2A-mCerulean3-pAAV-CaMKII
Plasmid#204971PurposeSoma-targeted neuronal expression of the MsACR1 mutant raACR and mCerulean3 separated by a P2A site.DepositorInsertraACR
UseAAVTagsmCerulean3ExpressionMutationPromoterCaMKIIAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUSE-Src-YF-UniRapR-mCerulean-myc
Plasmid#45381DepositorInsertSrc-YF-UniRapR-mCerulean-myc
UseTagsMyc, Myr, and mCeruleanExpressionMammalianMutationchanged mSrc Tyrosine 529 to PhenylalaninePromoterCMVAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
PhOTO-N (pMTB:memb-Cerulean-2A-H2B-Dendra2)
Plasmid#92400PurposeConstitutive expression of nuclear green-to-red photoconvertible Dendra2 and the membrane blue fluorescent protein Cerulean.DepositorInsertsDendra2
Cerulean
UseZebrafish expressionTagsH2B tag and membrane tagExpressionMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDUET-1-alpha-synuclein-mCerulean3-His6
Plasmid#110061PurposeFor bacterial expression of human alpha-synuclein, carboxyl terminally tagged with mCerulean3 and poly-histidinesDepositorInsertalpha-synuclein (SNCA Human)
UseTags6 x His and mCerulean3ExpressionBacterialMutationPromoterT7Available sinceMay 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
paavCAG-JxOFF-pre-mGRASP-mCerulean
Plasmid#51900PurposeJxOFF-preDepositorInsertpresynaptic mGRASP
UseAAV and Cre/LoxTagsExpressionMutationPromoterAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-ubb:MCP-p65-HSF1-2a-Cerulean-Ds
Plasmid#119074PurposeZebrafish ubiquitin promoter driving MCP-p65-HSF1 with Cerulean reporterDepositorInsertubb:MCP-p65-HSF1-2a-Cerulean
UseTagsExpressionBacterialMutationPromoterZebrafish ubiquitin promoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2plus-mCerulean 156-239-GGGS
Plasmid#162616PurposeAllows for transcription of control mCerulean fluorophore half for injection into zebrafish embryos for BiFC assaysDepositorInsertmCerulean
UseTagsExpressionMammalianMutation156-239PromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 mCerulean LIC cloning vector (6E)
Plasmid#30128DepositorTypeEmpty backboneUseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
PhOTO-M (pMTB:memb-Dendra2-2A-H2B-Cerulean)
Plasmid#92401PurposeConstitutive expression of membrane green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.DepositorInsertsDendra2
Cerulean
UseZebrafish expressionTagsH2B tag and membrane tagExpressionMutationPromoterbactin2Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUSE-Src-KD-UniRapR-mCerulean-myc
Plasmid#45382DepositorInsertSrc-KD-UniRapR-mCerulean-myc
UseTagsmCerulean and mycExpressionMammalianMutationmSrc R388D (kinase dead), Y529FPromoterCMVAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
Ef1a-somBiPOLES-mCerulean-IRES-Puro
Plasmid#192585Purposelentiviral vector for bidirectional optogenetic manipulation of Cre-positive neuronsDepositorInsertsomBiPOLES
UseLentiviralTagssoma-targeting motif from Kv2.1 channelExpressionMammalianMutationPromoterEf1-alphaAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-mCerulean3-cpVenus[E172]
Plasmid#125203PurposeSB-transposon plasmid for stable expression of mCerulean3-cpVenus[E172] FRET high controlDepositorInsertmCerulean3-cpVenus[E172]
UseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT-ubb-Avi-Cerulean-Rpl10a
Plasmid#79882PurposeUbiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
UseTagsAviExpressionBacterialMutationPromoterUbiquitinAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mCerulean-PA-Rac1
Plasmid#22031DepositorInsertmCerulean-PA-Rac1 (RAC1 Avena sativa (oat), Human)
UseRetroviralTagsExpressionMammalianMutationRac1 starts at I4 and contains mutations Q61L, E9…PromoterAvailable sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only