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  2. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...F64L; S65T, Y66W; N146I; M153T; V163A; A206K Cerulean F64L, S65T, Y66W, S72A, Y145A, H148D, N149I, M153T... Y145A and H148D Stabilizes the structure of Cerulean derivatives F99S, M153T, V163A Improves folding...

  3. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    ... fused to the N-terminus of monomeric Cerulean mCerulean C1 Cyan Mammalian Express a gene of interest...interest fused to the C-terminus of monomeric Cerulean mTurquoise2 Cyan Mammalian Constructs to target mTurquoise2...been used since - including: mCerulean/mVenus, mCerulean/Amber, mCerulean/SYFP2A, mTurquoise/mVenus and...Mammalian Expresses mammalian optimized Aquamarine mCerulean N1 Cyan Mammalian Express a gene of interest fused...

  4. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...Plasmid FRET Pair C5V Cerulean attached to Venus via a 5 amino acid linker C17V Cerulean attached to Venus... acid linker C32V Cerulean attached to Venus via a 32 amino acid linker C5A Cerulean attached to Amber...acid linker C17A Cerulean attached to Amber via a 17 amino acid linker C32A Cerulean attached to Amber...linker-Cerulean-6aa linker-Amber ACV Heterotrimeric construct consisting of Amber-5aa linker-Cerulean-6aa...linker-Cerulean-6aa linker-Amber VCV Heterotrimeric construct consisting of Venus-5aa linker-Cerulean-6aa... fused to the N-terminus of monomeric Cerulean mCerulean C1 Cyan Mammalian Express a gene of interest ...interest fused to the C-terminus of monomeric Cerulean mTurquoise2 Cyan Mammalian Constructs to target mTurquoise2...

  6. MXS Chaining

    Type
    Blog Post
    ...TagBFP 399nm/ 456nm histone 2B (H2B) Chromatin 2 Cerulean 433nm/ 475nm Lyn-tag (derived from the tyrosine-protein...

  7. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression Cerulean 433 475 27 4.7 Monomer (A206K) Cerulean - Mammalian Expression pCS2+8CCerulean - Zebrafish... Plasmids - Set includes tagging with mCherry, Cerulean, Citrine, and eGFP pPD95_75 - C-terminal GFP for...cloning; includes tagging with ECFP, EYFP, DsRed, Cerulean Bacteria Gradia Lab Bacterial Vectors - Includes...Expression mCerulean C1 - Mammalian Expression mCerulean3 433 475 32 3.2 Monomer (A206K) mCerulean3-N1 - Mammalian...Expression mCerulean3-C1 - Mammalian Expression moxCerulean3 434 474 36 3.2 Monomer (A206K) moxCerulean3 - Mammalian...C.elegans/Sea urchin pCS2+8NCerulean - Zebrafish/Xenopus/C.elegans/Sea urchin mCerulean N1 - Mammalian Expression...Plasmids - Includes tagging with mCherry, mCitrine, mCerulean Davidson Lab Plasmids - Includes many N- and C-terminal...

  9. Cre-lox system

    Type
    Collection
    ...24334 hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean Heat-inducible reporter with Cre dependent H2B-RFP...

  13. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...C-terminal tags for mTagBFP, TagRFPt, EGFP, mVenus, mCerulean3, mKOFP2) and empty vectors that have standard...

  15. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...sequence and COX VIII signal peptide dL5 FAP; mCerulean3 Marcel Bruchez 44385 pLV-mitoGFP Mitochondria...2xG4S-mCer3 Nucleus NLS (from Mak16p protein) dL5 FAP; mCerulean3 Marcel Bruchez 73207 pcDNA3.1-KozATG-dL5-2XG4S-mCer3...2XG4S-mCer3 Cytosol, nucleoplasm None dL5 FAP; mCerulean3 Marcel Bruchez 36206 pmTurquoise2-NES Non-nucleus...Cell surface (mammalian) PDGFR-derived dL5 FAP; mCerulean3 Marcel Bruchez 45944 pTDpelB-C_sfYFPTwinStrep...tripeptide, peroxisome transport signal dL5 FAP; mCerulean3 Marcel Bruchez 85065 pmScarlet-I_peroxisome_C1...2XG4S-mCer3-KDEL Endoplasmic Reticulum KDEL dL5 FAP; mCerulean3 Marcel Bruchez 73209 pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3...2XG4S-mCer3-KDEL Endoplasmic Reticulum KDEL dL5 FAP; mCerulean3 Marcel Bruchez 85068 pCytERM_mScarlet-i_N1 Endoplasmic...

  16. Optogenetics AAV Preps

    Type
    Collection
    ... 154948 CaMKII-somBiPOLES-mCerulean CamKII BiPOLES (soma-targeted) mCerulean Constitutive 5, 9, rg* Wiegert...154951 hSyn-DIO-somBiPOLES-mCerulean Syn BiPOLES (soma-targeted) mCerulean Cre dependent 5, 9, rg* Wiegert...

  17. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pCS2plus-mCerulean 156-239-GGGS-TDP-43 TARDBP mCerulean CMV ALS Marco Morsch 162618 pCS2plus-mCerulean 156-...Parkinson's, FTD Michael Davidson 55431 mCerulean3-MAPTau-C-10 MAPT mCerulean3 CMV Parkinson's, FTD Michael Davidson...Sidhu 110061 pDUET-1-alpha-synuclein-mCerulean3-His6 SNCA His, mCerulean3 T7 Parkinson's Dan Mulvihill 110486...Lippincott-Schwartz 164214 PLEX-PGK-ANXA11-mCerulean ANXA11 mCerulean PGK ALS Jennifer Lippincott-Schwartz ...mRuby CMV ALS Jennifer Lee 216227 TDP-43 mCerulean TARDBP mCerulean CMV ALS Jennifer Lee 216843 pET-Duet_...156-239-GGGS-TDP-43 M337V TARDBP mCerulean CMV ALS Marco Morsch 163622 pDEST-eGFP-C1-VPS35 VPS35 GFP CMV...

  18. Control AAV Preps

    Type
    Collection
    ...Constitutive 9 Wilson 105557 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG CB7 mCerulean Constitutive 1, 9 Wilson 105598 pAAV.GfaABC1D.PI.Lck-GFP.SV40...

  19. Retrograde AAV viral preps

    Type
    Collection
    ...CaMKII-somBiPOLES-mCerulean CamKII Bidirectional Optogenetics Wiegert 154951 hSyn-DIO-somBiPOLES-mCerulean Syn Bidirectional...

  20. Penn Vector Core Partnership with Addgene

    Type
    Collection
    ...Deisseroth AV-1-PV3248 105557-AAV1 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG James M. Wilson AV-1-PV3508 98928-AAV1 pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40...Wickersham AV-9-PV3248 105557-AAV9 pENN.AAV.CB7.CI.mCerulean.WPRE.RBG James M. Wilson Cre Recombinase AV-1-PV1090...
Showing: 1 - 20 of 20 results