-
Plasmid#110076PurposeAnalogous to pMINTNH3 but with a C-terminal 3C cleavage site and a GFP-His10 tag fusion.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only
-
pGL4-Gateway-SCP1
Plasmid#71510PurposehumanSTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the SCP1 core promoterDepositorTypeEmpty backboneUseHumanstarr-seq validation vectorTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCRISP_IS
Plasmid#120425PurposeThis plasmid encodes the complete CRISPR/dCas9 machinery for repressing transposition of the following bacterial Insertion Sequences: IS1, IS3 and IS5.DepositorInsertCRISPR spacers targeting IS1, IS5, IS3
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterconstitutiveAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB_init_Sb92
Plasmid#159424PurposeExpression of the anti-TGP (thermostable GFP) synthetic nanobody Sb92DepositorInsertSb92
UseTagsc-Myc and poly-HisExpressionBacterialMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL4.23-SCP1-ccdB
Plasmid#131628PurposeModified STARR-seq plasmid to measure enhancer activity (SCP1 promoter; ccdb gene at cloning site)DepositorTypeEmpty backboneUseLuciferaseTagsExpressionBacterial and MammalianMutationPromoterSCP1Available sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
p15aC-1D-5
Plasmid#107865PurposeBacterial expression of PIS and PI4P5KDepositorInsertsPIS
phosphatidylinositol 4-phosphate 5-kinase
UseTagsmycExpressionBacterialMutationPromoterproD and proD (in operon)Available sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pdestMB14
Plasmid#26415DepositorInsertattR4-ccdB-attR2
UseTagsExpressionBacterial and WormMutationPromoterAvailable sinceApril 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pOPO635
Plasmid#195297PurposepACYC-plac-MycaTnsABC, lac promoter expressing transposase genes TnsABC of McCAST (Tn7575).DepositorInsertTnsABC operon of McCAST (Tn7575)
UseTagsExpressionBacterialMutationPromoterIPTG inducible lac promoterAvailable sinceMay 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046959
Plasmid#145722PurposeBacterial Expression plasmid for SARS-CoV-2 ORF3a proteinDepositorInsertSARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQCXIZ CMV/TO DEST (w321-2)
Plasmid#17401PurposeRetroviral Gateway destination vector, Expression, CMV/TO promoter, ZeoDepositorTypeEmpty backboneUseRetroviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLENTI_RPS10_K138/139R
Plasmid#127138DepositorInsertRPS10 (RPS10 Human)
UseLentiviralTagsExpressionMutationK138R/K139RPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046879
Plasmid#149238PurposeBacterial expression plasmid for SARS-CoV-2 nsp2DepositorInsertSARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJB089
Plasmid#98914PurposeExpress Dronpa tagged ZapA and pAmCherry1 tagged FtsZ in E coliDepositorInsertsFtsZ
ZapA
UseTagsDronpa and pAmCherry1ExpressionBacterialMutationPromoterT5-lacAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
CDS12_mTurquoise2
Plasmid#136089PurposeFP for N-term fusion with a CTAGDepositorInsertCDS12_mTurquoise2(noStop)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTU2-a-RFP
Plasmid#72958PurposeLevel 2 Destination vector - Position A - Assembly of 2 TU's from Level 1-ABDepositorInsertLevel 2 - a site
UseSynthetic BiologyTagsExpressionMutationCAT gene - C435G (nucleotide) - silent mutagenesi…PromoterAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
CDS_LUC
Plasmid#136083PurposeFirefly luciferaseDepositorInsertCDS_LUC
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046942
Plasmid#145757PurposeBacterial Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046764
Plasmid#145673PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;StrepIIExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pWS1837
Plasmid#123058Purpose_-factor pheromoneDepositorInsert_-factor [3-4a]
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR-P2rP4
Plasmid#141015PurposeMutisite Gateway mediated vector constructionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only