pdestMB14
(Plasmid
#26415)
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 26415 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDP#MM016b
- Backbone size w/o insert (bp) 8623
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Vector typeBacterial Expression, Worm Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Growth instructionsccdB resistant bacteria Ex: DB3.1 E. coli
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameattR4-ccdB-attR2
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Insert Size (bp)3479
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (unknown if destroyed)
- 3′ cloning site KpnI (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A KpnI-ApaI fragment containing the GFP encoding sequence and the unc-54 3′ UTR from pPD95.79 was cloned into pDP#MM016b. An EcoRV fragment of the attR4-ccdB-attR2 cassette from pDEST-6 (Invitrogen) was cloned into this new construct digested with Acc65I and filled in with Klenow.
The unc-119 rescuing fragment present in these vectors can be used as a marker to select transgenic worms.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pdestMB14 was a gift from Marc Vidal (Addgene plasmid # 26415 ; http://n2t.net/addgene:26415 ; RRID:Addgene_26415) -
For your References section:
A first version of the Caenorhabditis elegans Promoterome. Dupuy D, Li QR, Deplancke B, Boxem M, Hao T, Lamesch P, Sequerra R, Bosak S, Doucette-Stamm L, Hope IA, Hill DE, Walhout AJ, Vidal M.. Genome Res. 2004 Oct;14(10B):2169-75. 10.1101/gr.2497604 PubMed 15489340