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Plasmid#64799PurposeBacterial expression of His-tagged Grx1-roGFP2DepositorInsertGlutaredoxin-1 (GLRX Human)
UseTagsHis and roGFP2ExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-FLEx-axon-GCaMP6s
Plasmid#112010PurposeFor cre-dependent enriched expression of GCaMP6s in axonsDepositorHas ServiceAAV5 and AAV9Insertaxon-GCaMP6s
UseAAV and Cre/LoxTagsGAP43 palmitoylation domainExpressionMammalianMutationPromoterhSynapsin1Available sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tol2-kdrl:mCherry-caax
Plasmid#194287PurposeExpression plasmid for zebrafish or Danionella cerebrumDepositorInsertkdrl promoter from Addgene plasmid # 78687 (kdrl Zebrafish)
UseZebrafish or danionella cerebrum expressionTagsmCherryCAAX from the Tol2kit (Kwan et al., 2007)ExpressionMutationPromoterkdrl(flk1)Available sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUBC_HA_PP7-mVenus
Plasmid#111268PurposeTransient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tagDepositorInsertPP7-mVenus
UseSynthetic BiologyTagsHA, SV40 NLS, and mVenusExpressionMammalianMutationPP7: C68S, S69G [delta]70-75 (non-aggregating)PromoterhUBCAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_tKiMBI-T2A-caMEK
Plasmid#199578PurposeExpress tKiMBI and caMEK in an AAV vectorDepositorInsertsERK tdTomato-Kinase-Modulated Bioluminescent Indicator
constitutively active MEK
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG6f
Plasmid#62808PurposepPBC-LG6f expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6f under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP6f-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-Neuromodulin-IRES-Puromycin
Plasmid#134666PurposeExpresses EGFP-tagged plasma membrane markerDepositorInsertNeuromodulin (GAP43 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPBC-G3-tdT
Plasmid#62814PurposepPBC-G3-tdT expresses PiggyBac inverted terminal repeat-flanked GCaMP3 and tdTomato under the control of the CAG promoter.DepositorInsertITR-CAG-GCaMP3-IRES-tdTomato-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-IRES-puro-NLS-dRfxCas13d-EGFP-NLS-3xFlag
Plasmid#132411Purposeoverexpression in human cellsDepositorInsertdRfxCas13b
UseLentiviralTagsflagExpressionMammalianMutationR239A; H244A; R858A; H863APromoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
VN-beta Synuclein
Plasmid#89483Purposeexpresses human beta Synuclein fused to the N-terminal part of VenusDepositorInsertSNCB (SNCB Human)
UseTagsN-terminal part of VenusExpressionMammalianMutationPromoterCMVAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLight1.4
Plasmid#111057PurposeExpresses dLight1.4 in mammalian cellsDepositorInsertdLight1.4
UseTagsFlag tagExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
peGFP C1-SPG20 SC domain
Plasmid#218580PurposeExpresses GFP-tagged SC domain of human SPG20 in mammalian cellsDepositorInsertSPG20 (SPART Human)
UseTagseGFPExpressionMammalianMutationdeleted amino acids 1-417PromoterCMVAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m_tKiMBImut-T2A-AkaLuc-P2A-Blasticidin
Plasmid#199585PurposeExpress tKiMBImut(AA), AkaLuc and Blasticidin in a lentivirus vectorDepositorInsertsERK tdTomato-Kinase-Modulated Bioluminescent Indicator(mutant)
AkaLuc
Blasticidin
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-MAP1LC3B
Plasmid#175753PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-MAP1LC3BDepositorInsertMAP1LC3B (MAP1LC3B Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-AP-Sstr3-GFP-DEST
Plasmid#49098PurposeExpressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell linesDepositorInsertSstr3 (Sstr3 Mouse)
UseTagsGFP and acceptor peptide (AP)ExpressionMammalianMutationPromoterT7Available sinceNov. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
AY22_pgRNA.R5.1
Plasmid#100294PurposeCCR5-targeting gRNA expression plasmidDepositorInsertgRNA spacer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6 RNA Pol III promoter human snRNAAvailable sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FLAG-APEX2-LGALS1
Plasmid#175755PurposeLentiviral vector expressing N-terminally tagged APEX2-FLAG-LGALS1DepositorInsertLGALS1 (LGALS1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody
Plasmid#138128PurposeExpresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.DepositorInsertmClover3-anti-mouse-IgG(kappa)-nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody
Plasmid#138130PurposeExpresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.DepositorInsertmCherry-anti-mouse-IgG(kappa)-nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
tetO-PBX1
Plasmid#170699Purposedoxycycline-inducible overexpression of PBX1DepositorInsertPBX1 (PBX1 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only