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pHAGE-IRES-puro-NLS-dRfxCas13d-EGFP-NLS-3xFlag

Plasmid

#132411

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dRfxCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R239A; H244A; R858A; H863A

Promoter

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pDule2-Acd82

Plasmid

#174100

Purpose

Acridone82 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.

Depositor

Ryan Mehl

Article

Jones et al Chem Sci. 2021 Aug 3;12(36):11955-11964.

Insert

Acridone 82 tRNA synthetase and tRNA

Use

Tags

Expression

Bacterial

Mutation

N311S, C313G, V366A, W382T

Promoter

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody

Plasmid

#138128

Purpose

Expresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mClover3-anti-mouse-IgG(kappa)-nanobody

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pEF5B-FRT-AP-Sstr3-GFP-DEST

Plasmid

#49098

Purpose

Expressing AP-Sstr3-GFP in mammalian cells, can be used for establishing Flp-In stable cell lines

Depositor

Maxence Nachury

Article

Ye et al Elife. 2013 Aug 6;2:e00654. doi: 10.7554

Insert

Sstr3 (Sstr3 Mouse)

Use

Tags

GFP and acceptor peptide (AP)

Expression

Mammalian

Mutation

Promoter

T7

Available since

Nov. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag Yap1 Y357F

Plasmid

#18882

Depositor

Yosef Shaul

Article

Levy et al Mol Cell. 2008 Feb 15. 29(3):350-61.

Insert

Yap1 Y357F (YAP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Y357F

Promoter

Available since

Aug. 14, 2008

Availability

Academic Institutions and Nonprofits only

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pFH2.94_PB-TRE3GS-Gag-MCP-T2A-eGFP-PGK-Tet3G-T2A-Blast

Plasmid

#205540

Purpose

Express HIV Gag-MCP-T2A-eGFP in doxycycline-inducible manner, and constitutively express Tet3G transactivator and blasticidin resistance marker

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Gag-MCP

Use

Piggybac

Tags

Expression

Mammalian

Mutation

WT

Promoter

TRE3GS

Available since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pmiRFP703-Clathrin

Plasmid

#79997

Purpose

Clathrin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

Clathrin (CLTB Human)

Use

Tags

miRFP703

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody

Plasmid

#138130

Purpose

Expresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

Depositor

Kazuhisa Nakayama

Article

Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

Insert

mCherry-anti-mouse-IgG(kappa)-nanobody

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-FlaviA-GFP-puro

Plasmid

#140088

Purpose

Fluorescence activatable reporter of flavivirus NS2B-NS3 protease activity

Depositor

Jorge Luis Arias-Arias

Article

Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

Insert

FlaviA-GFP

Use

Lentiviral; Low expression

Tags

6XHis tag and GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-VARNAM2-Kv2.1PR

Plasmid

#195530

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV

Tags

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

CaMKII

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.Syn.NES-jRGECO1b.WPRE.SV40

Plasmid

#100857

Purpose

AAV expression of jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

Has Service

AAV1

Insert

jRGECO1b

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-mCherry-WPRE

Plasmid

#202620

Purpose

Double floxed red fluorescent protein mCherry in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

mCherry

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pFUSEss-CHIg-mG2b_M18

Plasmid

#105931

Purpose

Expression plasmid coding for heavy chain of mouse IgG2b antibody specific to antigen B of the ABO blood group system, clone M18.

Depositor

Joanna Bereta

Article

Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

Insert

IgG2b (Ighg2b Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

hEF1-HTLV prom

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pSW003-Pc-mNeonGreen

Plasmid

#205018

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mNeonGreen (codon optimized for P. fluorescens)

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

mNeonGreen

Use

Tags

Expression

Bacterial

Mutation

mNeonGreen is codon optimized for expression in P…

Promoter

Pc

Available since

Sept. 15, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor

Plasmid

#86843

Purpose

HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.

Depositor

Thomas Cech

Article

Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d

Insert

FLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)

Use

Tags

FLAG-HaloTag

Expression

Mammalian

Mutation

Promoter

Endogenous TERT promoter

Available since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD-eLACCO2.1

Plasmid

#208017

Purpose

Biosensor for extracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

eLACCO2.1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-VARNAM2-Kv2.1PR

Plasmid

#195524

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

Depositor

Vincent Pieribone

Article

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

VARNAM2-Kv2.1 proximal restriction sequence

Use

AAV

Tags

Expression

Mammalian

Mutation

VARNAM SI linker

Promoter

Synapsin

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_eCB2.0mut

Plasmid

#164607

Purpose

Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in Cre positive neurons

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid non-responsive sensor GRAB_eCBmut

Use

AAV

Tags

Expression

Mutation

S383T, F177A

Promoter

hSyn

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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tetO-SMAD6

Plasmid

#170700

Purpose

doxycycline-inducible overexpression of SMAD6

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

SMAD6 (SMAD6 Human)

Use

Lentiviral; Doxycycline inducible

Tags

Expression

Mammalian

Mutation

Promoter

TRE promoter, Tet-ON

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dAdmCas13d-EGFP-NLS-3xFlag

Plasmid

#132412

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dAdmCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R269A; H274A; R837A; H842A

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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