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Viral Prep#130998-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE (#130998). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE plasmid DNA. Ef1a-driven, Cre-dependent expression of ChRmine-mScarlet. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagsmScarletAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only
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EF1a-dCas9-KRAB-GFP backbone
Plasmid#194281PurposeVector for CRISPRi-GFP expression ready for gateway cloning of sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterEF1aAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pBHRSF184
Plasmid#89482PurposeExpress asparaginyl endopeptidase 1 from O. affinis (OaAEP1)DepositorInsertAsparaginyl endopeptidase from Oldenlandia affinis
UseTagsSmt3ExpressionBacterialMutationPromoterT7Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE16
Plasmid#90357PurposeCREB - Cyclic AMP gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterCREBAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
SIN40C.SFFV.Luciferase.IRES.GFP
Plasmid#169308PurposeConstitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent proteinDepositorInsertLuciferase
UseLentiviralTagsIRES-EGFPExpressionMutationPromoterSFFVAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-mt-mKeima-P2A-FRB-Fis1
Plasmid#135295Purposestably express both mKeima and FRB-Fis1 for CID induced mitophagy in mammalian cellsDepositorInsertmt-mKeima-P2A-FRB-Fis1 (FIS1 Human)
UseLentiviralTagsExpressionMammalianMutationmt-mKeima is fused with FRB-Fis1 via P2A seqeuncePromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-LC3B WT
Plasmid#123092PurposeExpresses 3xFLAG-LC3B wild-type in mammalian cells.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-10His-mStayGold (E138D)
Plasmid#211363PurposeMammalian expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Contains a N-terminal 10xHis tag.DepositorInsertmStayGold
UseTags10xHis-tagExpressionMammalianMutationE138DPromoterCMVAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRDA_550
Plasmid#203398PurposeAll-in-one lentiviral expression of gRNA and EnAsCas12aDepositorInsertEnhanced Acidaminococcus Cas12a
UseCRISPR and LentiviralTagsExpressionMutationPromoterEF1aAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NOD2-EGFP
Plasmid#131207Purposemammalian expression, tet RDepositorInsertNOD2 (NOD2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterpCMVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-IRES-Puro
Plasmid#85132Purposelentiviral backboneDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST (AAV9)
Viral Prep#105448-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST (#105448). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST plasmid DNA. hSyn-driven expression of ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmRuby2Available sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMP0.4-FHS-paCaMKII(SD)-WPRE3
Plasmid#165430PurposeExpresses light-insensitive photoactivatable CaMKII(SD: Super Dark) in mammalian cells as a negative control.DepositorInsertFHS-paCaMKII(SD)
UseAAVTagsFHS(Flag, HisX6, Strep) tagExpressionMammalianMutationC450A, L514K, G528A, L531E, N538E: Light-insensit…PromoterCaMKII0.4Available sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-R-iLACCO1.2
Plasmid#208101PurposeBiosensor for intracellular L-lactateDepositorInsertR-diLACCO1
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tet-on circRNA Expression Vector
Plasmid#92351PurposeThe vector can ef_ciently produce a circRNA (circmCherry) from a split EGFP gene after Dox treatment.DepositorInsertmCherry and split EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterpTetAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-tdTomato (codon diversified)
Plasmid#59462PurposeAAV-mediated expression of tdTomato (codon diversified) under the CAG promoterDepositorHas ServiceAAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InserttdTomato
UseAAVTagsExpressionMammalianMutationcodon diversifiedPromoterCAGAvailable sinceSept. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV-polysite
Plasmid#122843PurposeGateway destination vector for mammalian expression without tag, or for adding custom tags by conventional cloning. Distinct multiple cloning sites 5' and 3' of gateway cassette.DepositorTypeEmpty backboneUseGateway destinationTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH014_AAVS1_puro-pA-HS4-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry
Plasmid#179436PurposeDual fluorescent reporter construct with double HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locusDepositorInsertHS4-TRE-cit-HS4-mch (DH)
UseTagsExpressionMammalianMutationPromoterpEF alpha, pRSVAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-R-diLACCO1
Plasmid#208102PurposeControl biosensor for intracellular L-lactateDepositorInserteLACCO2.1
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only