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Plasmid#186542PurposeLSSmGFP fluorescent protein (ex/em = 400/510 nm): bacterial expression plasmidDepositorInsertLSSmGFP
UseTags6xHisExpressionBacterialMutationhfYFP-T43S/G65S/L68Q/H77N/K140N/Y203I/V206KPromoteraraBAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAJM.336
Plasmid#108528PurposeLacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.DepositorInsertLacIAM + PTac-YFP
UseTagsExpressionBacterialMutationLacI Q18M A47V F161YPromoterAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAHA
Plasmid#12517DepositorTypeEmpty backboneUseTagsDouble HA tagExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pNCS BFP
Plasmid#91757PurposeBacterial expression of a fluorescent protein, Blue Fluorescent Protein (BFP)DepositorInsertBlue Fluorescent Protein
UseTagsHexa-Histidine, Xpress epitope for detection with…ExpressionBacterialMutationGFP (Y66H)PromoterT7Available sinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST_6xUAS:mNeonGreen-Rab5a
Plasmid#192355PurposezfRabaa tagged with mNeonGreen, expressed under the UAS promoterDepositorInsertRab5a (rab5aa Zebrafish)
UseTagsmNeonGreenExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLCS104
Plasmid#209357PurposeGateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal HaloTag fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EFS-PCP-GFPnls
Plasmid#121938PurposeCRISPR-Sirius plasmidDepositorInsertPCP-GFPnls
UseLentiviralTagssfGFPExpressionMammalianMutationPromoterEFSAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
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CMV-HA-hTAF6
Plasmid#17988DepositorInsertTAF6 (TAF6 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJRA99
Plasmid#209334PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of untagged protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pGEMHE-mEGFP-mTrim21
Plasmid#105519PurposeT7 promotor drives in vitro transcription of mEGFP-tagged wildtype mouse TRIM21 mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmEGFPExpressionMutationPromoterT7Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGxxFP
Plasmid#50716Purpose5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.DepositorInsertsplit EGFP
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ140
Plasmid#99895PurposeEmpty vector with attL5-attL2 recombination sites for Gateway CloningDepositorTypeEmpty backboneUseCRISPR and TALENTagsExpressionMutationPromoterAvailable sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAJM.657
Plasmid#108525PurposeCymRAM + PCymRC-YFP Reporter. Cuma sensor for use in non-Marionette strain.DepositorInsertCymRAM + PCymRC-YFP
UseTagsExpressionBacterialMutationCymR: S110G A171VPromoterAvailable sinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD81-(TEV)-Cre
Plasmid#167939Purposehuman CD81-TEV cleavage site-CreDepositorInserthuman CD81 (CD81 Human)
UseTagsCre recombinase and TEV protease cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-FLAG-CHK1
Plasmid#86875PurposeTo express CHK1 with a FLAG tag at N-terminus.DepositorInsertCHEK1 (CHEK1 Human)
UseTagsFLAGExpressionMammalianMutationnonePromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHIV-H2B-eGFP
Plasmid#91776PurposeExpresses H2B protein tagged with eGFPDepositorInsertHuman Histone H2B / eGFP (H2BC11 Human)
UseLentiviralTagseGFPExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCFD5-frame_selector_0,1,2
Plasmid#131152PurposeDrosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-inDepositorInsertCRISPaint frame selector sgRNAs 0,1,2
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hc-MYC
Plasmid#17220PurposeRetroviral expression of human cMYCDepositorInsertc-MYC (MYC Human)
UseRetroviralTagsExpressionMammalianMutationNonePromoterAvailable sinceFeb. 8, 2008AvailabilityAcademic Institutions and Nonprofits only