Addgene

We narrowed to 6,237 results for: mag

Showing: 3241 - 3260 of 6237 results

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pKanCMV-CX4.3-7aa-mClover3

Plasmid

#74260

Purpose

Mammalian expression of C-terminal fusion of mClover3 to CX43

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

Connexin 43

Use

Tags

mClover3

Expression

Mammalian

Mutation

Promoter

CAG and CMV

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dPspCas13b-mNeongreen-NLS-3xFlag

Plasmid

#132401

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dPspCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

H133A; H1059A

Promoter

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-TorA(sp)

Plasmid

#111253

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorA signal peptide

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

TorA signal peptide

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pc

Available since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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KTK_251

Plasmid

#180546

Purpose

Entry vector containing mScarlet

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

mScarlet

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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piGECI-NES-N1

Plasmid

#160422

Purpose

Expression of a near-infrared genetically encoded calcium indicator iGECI with a nuclear export signal sequence

Depositor

Vladislav Verkhusha

Article

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI-NES

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-GCaMP6s-P2A-mKate2

Plasmid

#112006

Purpose

For co-expression of GCaMP6s with mKate2

Depositor

Lin Tian

Article

Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

Has Service

AAV9

Insert

GCaMP6s-P2A-mKate2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSynapsin1

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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csr-1_iRFP670_son-1_GpdToxA_bml_mTagBFP2

Plasmid

#191751

Purpose

Microtubule marker together with nuclear envelope marker driven by bidirectional promoter

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Inserts

NCU04288 (NCU04288 Neurospora crassa)
bml (Bml Neurospora crassa)

Use

Tags

Gdp1 promoter, ToxA promoter, iRFP670, and mTagBF…

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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SL2-sgTelo-MTSa/EGFP/pdCas9-C1

Plasmid

#162759

Purpose

Expressing dCas9 and sgRNA containg MTSa targeting telomeres

Depositor

Antony K Chen

Article

Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

Insert

dCas9 and sgRNA(SL2-sgTelo-MTSa)

Use

Tags

Expression

Mammalian

Mutation

dCas9(nuclease deactivated Cas9)

Promoter

U6/CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6015nes

Plasmid

#108657

Purpose

A S6 Kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

hyBRET-S6K-EV

Use

Tags

XFP

Expression

Mammalian

Mutation

Promoter

pCAGGS

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pBEST_OR2-OR1-Pr-UTR1_ClpP-T500

Plasmid

#153302

Purpose

Expresses ClpP protease in the cell-free TX-TL system

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

clpP (clpP Escherichia coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6017nes

Plasmid

#108655

Purpose

A PKA biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

hyBRET-PKA-EV

Use

Tags

XFP

Expression

Mammalian

Mutation

Promoter

pCAGGS

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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mOrange2-Actin-7

Plasmid

#57943

Purpose

Localization: Actin, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

Actin (ACTB Human)

Use

Tags

mOrange2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 22, 2014

Availability

Academic Institutions and Nonprofits only
pvimentin-emiRFP703

Plasmid

#136566

Purpose

Vimentin labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

vimentin-emiRFP703

Use

Tags

emiRFP703

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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BAS285 C terminal mKO2 hygBR

Plasmid

#74080

Purpose

S.pombe recoded mKO2 in universal C-terminal tagging plasmid, hygB resistance.

Depositor

Bassem Al-Sady

Article

Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

Insert

mKO2

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only
pICE-EGFP-FLAG-Ku70siR-WT

Plasmid

#46961

Purpose

Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

human Ku70 (XRCC6 Human)

Use

Tags

GFP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV Tet

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pJR2956

Plasmid

#122533

Purpose

Expression of a C. elegan codon optimized fluorescent protein (CemOrange2) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_CemOrange2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pnhx-2

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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SL2-sgSat-MTSb/BFP/pdCas9-C1

Plasmid

#162761

Purpose

Expressing dCas9 and sgRNA containg MTSa targeting centromeres

Depositor

Antony K Chen

Article

Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

Insert

dCas9 and sgRNA(SL2-sgSat-MTSb)

Use

Tags

Expression

Mammalian

Mutation

dCas9(nuclease deactivated Cas9)

Promoter

U6/CMV

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-Pc-DsRed-Express2

Plasmid

#111260

Purpose

mini-Tn7 vector for insertion of DsRed-Express2 at bacterial attTn7 site

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

DsRed-Express2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Pc

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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Stx3-pmCitrine

Plasmid

#92421

Purpose

Expression of syntaxin-3 C-terminally conjugated to mCitrine.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

Stx3 (Stx3 Rat)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pUC18T-mini-Tn7T-Gm-Pc-mTurquoise2

Plasmid

#111259

Purpose

mini-Tn7 vector for insertion of mTurquoise2 (codon optimized for P. fluorescens) at bacterial attTn7 site

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

mTurquoise2

Use

Tags

Expression

Bacterial

Mutation

mTurquoise2 is codon optimized for expression in …

Promoter

Pc

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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