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Plasmid#125032PurposeStorage plasmid for K.marxianus left homology arm; type 1 partDepositorInsertLeft homology arm for targeting integration to SWF1/ARO1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACYCT2
Plasmid#45799DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTH103
Plasmid#84461PurposepJB38-NWMN29-30 + SarA_P1-YFP-TermDepositorInsertSarA_P1-YFP from pTH3
UseTagsExpressionMutationPromoterAvailable sinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSAP
Plasmid#206429PurposeA vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCas9CyMutL
Plasmid#166981PurposeEncodes aTc-inducible spCas9 and cumate-inducible MutLE32K for transient suppression of mismatch repairDepositorInsertsUseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationE32KPromoterpCymRC and pLacIAvailable sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
p15A-OptoCre-knt-P-R
Plasmid#189849PurposeCre-inducible activation of knt for kanamycin resistance.DepositorInsertP-loxP-TT-loxP-R-knt
UseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterP (T7 phage gene 10)Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKm-HCBE
Plasmid#170333Purposea modularized single-plasmid cytidine base editing (C-to-T) tool for Sinorhizobium melilotiDepositorInsertRhizobium codon-optimized CBEs
UseCRISPRTagsExpressionMutationCas9n-D10APromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVoPo-01
Plasmid#187836PurposeBackbone for transcriptional reporter system in Fusobacterium nucleatumDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAA3
Plasmid#128280Purposeasymmetric plasmid partitioning E. coli - target plasmidDepositorInsertPiq - mRFP, parS
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCC28
Plasmid#128284Purposeasymmetric plasmid partitioning E. coli - target plasmidDepositorInsertPiq - mRFP, sopC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKm-HGBE
Plasmid#170334Purposea modularized single-plasmid glycosylase base editing (C-to-G) tool for Sinorhizobium melilotiDepositorInsertRhizobium codon-optimized GBEs
UseCRISPRTagsExpressionMutationCas9n-D10APromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTD103aiiA(Cm)
Plasmid#48886PurposeNegative feedback plasmid from oscillatorDepositorInsertaiiA and LuxR
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialMutationPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
p2-39
Plasmid#118086PurposeZB-linker-split C-terminal T7 RNAP expression plasmidDepositorInsertZB-linker-split C-terminal T7 RNAP expression plasmid
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDest-623 (attR4-attR3)
Plasmid#161878PurposeMultisite Gateway (attR4-attR3) vector for expression of multiple proteins using the Bac-to-Bac insect cell systemDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRosa26-DEST
Plasmid#21189PurposeRosa26 knock-in vector for Cre-regulated cDNA expression and RNAi in transgenic miceDepositorInsertGateWay conversion cassette
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 2, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEZY19
Plasmid#18668PurposepET19b converted Gateway Destination vector for expressing His tagged fusion proteins in bacteria. See note in comments section below regarding the mutated enterokinase site.DepositorTypeEmpty backboneUseGateway destination vectorTagsHisExpressionBacterialMutationPromoterT7Available sinceJuly 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
MSCV-N-Flag-HA-IRES-PURO
Plasmid#41033DepositorTypeEmpty backboneUseRetroviral; Gateway destination vectorTagsFlag, HA, and IRES PuroExpressionMammalianMutationPromoterLTR-driven expressionAvailable sinceOct. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pJF093
Plasmid#113323PurposeSp.pCas9-dCas9, pTet-MCP-SoxS_R93ADepositorInsertdCas9 and MCP-SoxS_R93A
UseCRISPRTagsExpressionBacterialMutationR93APromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDest-eGFP-N1
Plasmid#31796DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pINTO_7/8
Plasmid#206431PurposeA vector backbone (pCC1BAC-based) containing a single SapI recognition site. When digested, it exposes overhangs that allow integration of the vector into the P. tricornutum chloroplast genome.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only