Skip to main content
Addgene
Showing: 301 - 320 of 3027 results

  1. pET-hMICU1EF

    Plasmid
    #199180
    Purpose
    This plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.
    Depositor
    Ming-Feng Tsai
    Article
    Tsai et al Proc Natl Acad Sci U S A. 2023 Apr 18;12
    Insert
    MICU1 (MICU1 Human)
    Use
    Tags
    His6 and MBP, Thrombin site, TEV site
    Expression
    Mutation
    D421A, E432K, F433W, F453W
    Promoter
    Available since
    Aug. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    199180-plasmid-map-sequence-id-403144

    Enlarge

  3. pRSET-RCaMP1h

    Plasmid
    #42874
    Purpose
    a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby
    Depositor
    Loren Looger
    Article
    Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33
    Insert
    Red Fluorescent Calcium binding Protein
    Use
    Tags
    EK (Enterokinase) Recognition Site, His Tag (6x),…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 22, 2013
    Availability
    Academic Institutions and Nonprofits only
    42874-plasmid-map-sequence-id-58020

    Enlarge

  6. elavl3:SomaGCaMP7f

    Plasmid
    #158760
    Purpose
    Cell body-targeted GCaMP7f, under HuC promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Insert
    SomaGCaMP7
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    HuC promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158760-plasmid-map-sequence-id-310667

    Enlarge

  9. pAAV-Syn-SomaGCaMP7

    Plasmid
    #158759
    Purpose
    Cell body-targeted GCaMP7f, under synapsin promoter: GCaMP7f followed by a linker, and the EE-RR coiled coil motif (GCaMP7f-27-EE-RR). As bright as conventional GCaMP7f.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Insert
    SomaGCaMP7
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    synapsin promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158759-plasmid-map-sequence-id-310665

    Enlarge

  10. pAAV-Syn-SomaGCaMP6f2

    Plasmid
    #158756
    Purpose
    Cell body-targeted GCaMP6f, under synapsin promoter: GCaMP6f followed by a linker, and the EE-RR coiled-coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Insert
    SomaGCaMP6f2
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    synapsin promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158756-plasmid-map-sequence-id-310660

    Enlarge

  11. pAAV-CAG-SomaGCaMP6f2

    Plasmid
    #158757
    Purpose
    Cell body-targeted GCaMP6f, under CAG promoter: GCaMP6f followed by a linker, and the EE-RR coiled coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Has Service
    AAV8 and AAV9
    Insert
    SomaGCaMP6f2
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158757-plasmid-map-sequence-id-310662

    Enlarge

  12. pAAV-Syn-SomaGCaMP6f1

    Plasmid
    #158755
    Purpose
    Cell body-targeted GCaMP6f under synapsin promoter: GCaMP6f followed by a linker, the tail region of AnkyrinG, and an ER export motif (GCaMP6f-27-AnkTail-motif-ER2). Not as bright as SomaGCaMP6f2.
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Insert
    SomaGCaMP6f1
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    synapsin promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158755-plasmid-map-sequence-id-310657

    Enlarge

  13. pAAV-Syn-nullCoChR-GCaMP6f-Kv2.1

    Plasmid
    #158758
    Purpose
    Cell body-targeted GCaMP6f, under synapsin promoter: nullCoChR (the optogenetic protein CoChR mutated to have 0 current), followed by GCaMP6f and the KV2.1 motif (nullCoChR-GCaMP6f-Kv2.1).
    Depositor
    Edward Boyden
    Article
    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)
    Insert
    nullCoChR-GCaMP6f-Kv2.1
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    synapsin promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158758-plasmid-map-sequence-id-310664

    Enlarge

  15. pMJA149

    Plasmid
    #128524
    Purpose
    Expresses mutant Ber e 1 in Pichia
    Depositor
    Marcos Alcocer
    Insert
    mutant Ber e 1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    AOX1
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    128524-plasmid-map-sequence-id-251308

    Enlarge

  16. pMJA151

    Plasmid
    #128526
    Purpose
    Expresses mutant Ber e 1 in Pichia
    Depositor
    Marcos Alcocer
    Insert
    mutant Ber e 1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    AOX1
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    128526-plasmid-map-sequence-id-251271

    Enlarge

  17. pMJA159

    Plasmid
    #128534
    Purpose
    Expresses mutant Ber e 1 in Pichia
    Depositor
    Marcos Alcocer
    Insert
    mutant Ber e 1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    AOX1
    Available since
    Aug. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    128534-plasmid-map-sequence-id-251620

    Enlarge

  18. pMJA157

    Plasmid
    #128532
    Purpose
    Expresses mutant Ber e 1 in Pichia
    Depositor
    Marcos Alcocer
    Insert
    mutant Ber e 1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    AOX1
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    128532-plasmid-map-sequence-id-251881

    Enlarge

  19. pMJA156

    Plasmid
    #128531
    Purpose
    Expresses mutant Ber e 1 in Pichia
    Depositor
    Marcos Alcocer
    Insert
    mutant Ber e 1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    AOX1
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128531-plasmid-map-sequence-id-251737

    Enlarge

  20. pMJA155

    Plasmid
    #128530
    Purpose
    Expresses mutant Ber e 1 in Pichia
    Depositor
    Marcos Alcocer
    Insert
    mutant Ber e 1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    AOX1
    Available since
    July 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128530-plasmid-map-sequence-id-251733

    Enlarge

Showing: 301 - 320 of 3027 results