168,605 results

1. TriEx-4 DHX36

   Plasmid

   #68368

   Purpose

   Expresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.

   Depositor

   James Vaughn

   Article

   Vaughn et al J Biol Chem. 2005 Nov 18;280(46):38117-2

   Insert

   DHX36 (DHX36 Human)
   

   Tags

   Histidine

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV ie enhancer/promoter

   Available Since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pCHAC-mt-mKeima

   Plasmid

   #72342

   Purpose

   retrovirus construct for stable expressing mt-mKeima

   Depositor

   Richard Youle

   Article

   Lazarou et al Nature. 2015 Aug 20;524(7565):309-14. do

   Insert

   mt-mKeima
   

   Use

   Retroviral

   Available Since

   Jan. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-GRP78-Wild-Type

   Plasmid

   #235169

   Purpose

   Expresses Wild-Type GRP78 in mammalian cells

   Depositor

   Amy Shiu Lee

   Article

   Zhang et al J Biol Chem. 2010 May 14;285(20):15065-1

   Insert

   78-kDa glucose-regulated protein (HSPA5 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGreenFire1_E2F1RE

   Plasmid

   #112248

   Purpose

   expression of GFP and Luciferase under the control of E2F1 responsive element

   Depositor

   Simone Di Giovanni

   Insert

   E2F1RE (responsive element)
   

   Use

   Lentiviral

   Tags

   copGFP and luciferase

   Available Since

   March 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. 7M8

   Plasmid

   #64839

   Purpose

   This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.

   Depositor

   John Flannery, David Schaffer

   Article

   Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra

   Insert

   7M8 cap
   

   Use

   AAV

   Mutation

   7mer insertion in the AAV2 cap

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Syn-DIO-NaChBac-dTomato

   Plasmid

   #236252

   Purpose

   Cre dependent NaChBac-TdTomato expression

   Depositor

   Janelia Viral Tools

   Article

   Kosse et al Nature. 2024 Dec;636(8041):151-161. doi:

   Insert

   NaChBac-TdTomato
   

   Use

   AAV

   Tags

   TdTomato

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. gag-P3-pol

   Plasmid

   #211374

   Purpose

   Packaging plasmid for v3b PE-eVLP production

   Depositor

   David Liu

   Article

   An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

   Insert

   MMLVgag-P3-pol
   

   Expression

   Mammalian

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI D450A

   Plasmid

   #207953

   Purpose

   nuclease inactive control for TRF1FokI WT

   Depositor

   Roger Greenberg

   Article

   Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

   Insert

   TRF1 (TERF1 Human)
   

   Use

   Lentiviral

   Tags

   Flag, DD, ER, mCherry and FokI (D450A)

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. nuclear cpVenus control

   Plasmid

   #186790

   Purpose

   Identical to nuclear NAD+ biosensor, sans bipartite NAD+-binding domain modeled from bacterial DNA ligase

   Depositor

   Michael Cohen

   Article

   Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

   Insert

   nuclear cpVenus control
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)

    Plasmid

    #196703

    Purpose

    Plasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.

    Depositor

    Karlene Cimprich

    Article

    Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

    Insert

    human RNaseH1 D210N
    

    Expression

    Mammalian

    Mutation

    D210N, catalytically inactive

    Available Since

    Feb. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-MRLC1

    Plasmid

    #35680

    Depositor

    Tom Egelhoff

    Article

    Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

    Insert

    MRLC1 (MYL9 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

12. C1(1-29)-TurboID-V5_pCDNA3

    Plasmid

    #107173

    Purpose

    expresses V5-tagged TurboID on the mammalian ER membrane

    Depositor

    Alice Ting

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID (BirA mutant)
    

    Expression

    Mammalian

    Mutation

    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-dLight1.3b (AAV5)

    Viral Prep

    #125560-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-CAG-dLight1.3b (#125560). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.3b plasmid DNA. CAG-driven expression of dLight1.3b dopamine sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Lin Tian

    Article

    Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

    Promoter

    CAG

    Available Since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

14. pCutamp

    Plasmid

    #140632

    Purpose

    Plasmid-curing in Escherichia coli by targeting the AmpR promoter

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    SpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. lentiCRISPRv2 hygro

    Plasmid

    #98291

    Purpose

    Variant of lentiCRISPRv2 that confers hygromycin resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Promoter

    EF-1a

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. FLAG-SFPQ

    Plasmid

    #166960

    Purpose

    Expresses FLAG-tagged SFPQ protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    SFPQ (SFPQ Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-CMV-Puro-AAVR-FLAG

    Plasmid

    #166716

    Purpose

    Vector expressing AAVR (KIAA0319L) with a C-terminal Flag tag

    Depositor

    Jan Carette

    Article

    Pillay et al Nature. 2016 Feb 4;530(7588):108-12. doi

    Insert

    AAVR-FLAG (KIAA0319L Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas9-CR4

    Plasmid

    #62655

    Purpose

    Cas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetR

    Depositor

    Kristala Prather

    Article

    Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

    Insert

    Cas9 nuclease
    

    Expression

    Bacterial

    Available Since

    Oct. 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-flex-taCasp3-TEVp (AAV8)

    Viral Prep

    #45580-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-flex-taCasp3-TEVp (#45580). In addition to the viral particles, you will also receive purified pAAV-flex-taCasp3-TEVp plasmid DNA. EF1a-driven, Cre-dependent, bicicstronic expression of designer pro-taCasp3 and TEVp. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Nirao Shah, Jim Wells

    Article

    Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

    Promoter

    EF1a

    Available Since

    Nov. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

20. LHZ1494

    Plasmid

    #222104

    Purpose

    Triple sgRNAs expression plasmid in Kluyveromyces marxianus.

    Depositor

    Yongming Wang

    Insert

    sg1RNA, sg4RNA, sg7RNA
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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