200,842 results

1. pHAGE-E-cadherin-RFP

   Plasmid

   #79603

   Purpose

   pHAGE lentiviral vector with dsRed driven by the human E-cadherin promoter. When used in combination with FUGW-d2GFP-ZEB1 3' UTR, comprises the Z-cad dual sensor. Use 2nd generation packaging system

   Depositor

   Jeffrey Rosen

   Article

   Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

   Insert

   CDH1 promoter region (CDH1 Human)
   

   Use

   Lentiviral

   Available Since

   July 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUW mCherry-GFP-LC3

   Plasmid

   #110060

   Purpose

   To visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)

   Depositor

   Anne Brunet

   Article

   Leeman et al Science. 2018 Mar 16;359(6381):1277-1283

   Insert

   mCherry-GFP-LC3
   

   Use

   Lentiviral

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Nb-gp41-GFP (MoonTag-Nb-GFP)

   Plasmid

   #128602

   Purpose

   Expresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

   Depositor

   Marvin Tanenbaum

   Article

   Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

   Insert

   Nanobody_gp41
   

   Use

   Lentiviral

   Tags

   GB1 and sfGFP

   Promoter

   SFFV

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

   Plasmid

   #185919

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

   Promoter

   CMV and T7

   Available Since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-SFPQ

   Plasmid

   #166960

   Purpose

   Expresses FLAG-tagged SFPQ protein in pcDNA3.1

   Depositor

   Rosalind Segal

   Article

   Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

   Insert

   SFPQ (SFPQ Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBAD-I-SceI

   Plasmid

   #60960

   Purpose

   Plasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesis

   Depositor

   Nikolaus Osterrieder

   Article

   Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

   Insert

   I-SceI restriction enzyme
   

   Available Since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV.rTH.PI.Cre.SV40

   Plasmid

   #107788

   Purpose

   AAV vector expressing Cre from rTH promoter

   Depositor

   James M. Wilson

   Has Service

   AAV Retrograde and AAV9

   Insert

   Cre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   rTH

   Available Since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+) eGFP

   Plasmid

   #129020

   Purpose

   Expresses eGFP

   Depositor

   Jeremy Wilusz

   Article

   Xiao et al Nucleic Acids Res. 2019 Jul 3. pii: 5527

   Insert

   eGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pML104

   Plasmid

   #67638

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   single guide RNA expression cassette
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   pSNR52 and pTDH3

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIRESpuroN3xFLAG-UFSP2

    Plasmid

    #204553

    Purpose

    Expresses 3xFLAG-tagged UFSP2 in mammalian cells

    Depositor

    Masaaki Komatsu

    Article

    Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

    Insert

    UFSP2 (UFSP2 Human)
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-ERK1

    Plasmid

    #14747

    Depositor

    Rony Seger

    Article

    Yung et al J Biol Chem. 2001 Sep 21. 276(38):35280-

    Insert

    ERK1 (MAPK3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-ERK1

    Plasmid

    #14747

    Depositor

    Rony Seger

    Article

    Yung et al J Biol Chem. 2001 Sep 21. 276(38):35280-

    Insert

    ERK1 (MAPK3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. CaMKAR

    Plasmid

    #205315

    Purpose

    Fluorescent Reporter for CaMKII Activity

    Depositor

    Mark Anderson, Elizabeth Luczak

    Article

    Reyes Gaido et al Sci Transl Med. 2023 Jun 21;15(701):eabq

    Insert

    CaMKAR
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFCIV-CEACAM6

    Plasmid

    #149303

    Purpose

    lentiviral packaging vector for CEACAM6 expression.

    Depositor

    James Fleckenstein

    Article

    Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

    Insert

    CEACAM6 (CEACAM6 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    ubiquitin C

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ131B2.0

    Plasmid

    #99885

    Purpose

    Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU3

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1 pegRNA2 with trimmed attP

    Plasmid

    #222347

    Purpose

    pegRNA 2 plasmid used for PASSIGE-mediated Bxb1 attP installation

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    AAVS1 attP-installation pegRNA2
    

    Use

    CRISPR

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. LBR-mCherry

    Plasmid

    #172446

    Purpose

    Expression of nuclear envelope protein LBR with a C-terminal mCherry tag

    Depositor

    Stephen Royle

    Article

    Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    lamin B receptor (LBR Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLEX307-TMPRSS2-blast

    Plasmid

    #158458

    Purpose

    Constitutive lentiviral expression of human TMPRSS2.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    TMPRSS2 (TMPRSS2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. mEmerald-Sec61b-C1

    Plasmid

    #90992

    Purpose

    Expresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

    Insert

    mEmerald-Sec61b (SEC61B Synthetic, Human)
    

    Tags

    mEmerald (green fluorescence)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ142

    Plasmid

    #69294

    Purpose

    Golden Gate recipient and Gateway entry vector; assembly of 2 gRNAs

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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