169,069 results

1. pWUR790

   Plasmid

   #101723

   Purpose

   Synthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgo

   Depositor

   John van der Oost

   Article

   Swarts et al Nucleic Acids Res. 2015 May 26;43(10):51

   Insert

   PfAgo
   

   Tags

   Strep(II)-tag

   Expression

   Bacterial

   Promoter

   T7 Promotor

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC0043-PspCas13b crRNA backbone

   Plasmid

   #103854

   Purpose

   For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Has Service

   Cloning Grade DNA

   Insert

   U6-BbsI-BbsI-PspCas13b DR-polyT
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 28, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCRIS-PITChv2-dTAG-Puro (BRD4)

   Plasmid

   #91796

   Purpose

   PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.

   Depositor

   James Bradner, Behnam Nabet

   Article

   Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

   Insert

   FKBP_F36V-2xHA-P2A-Puro
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Phenylalanine 36 to valine in FKBP12

   Promoter

   Promotorless

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221_SLC16A3

   Plasmid

   #131899

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC16A3 (SLC16A3 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 11, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pTKEI-Tre-C04

   Plasmid

   #79754

   Purpose

   Single fluorescent protein biosensor for trehalose

   Depositor

   David Savage

   Article

   Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

   Insert

   Tre-C04
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   pTrc

   Available Since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-DIO-GRAB_ACh3.0

   Plasmid

   #121923

   Purpose

   express the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0 in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Jing et al Nat Methods. 2020 Sep 28. pii: 10.1038/s

   Insert

   GPCR activation based ACh sensor GRAB-ACh3.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. miReporter-CAG

   Plasmid

   #82478

   Purpose

   microRNA reporter relying on a bidirectional CAG-based promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

   Insert

   H2B-mCherry and H2B-Citrine. HygroR
   

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX-SRRM2-mCherry

   Plasmid

   #174089

   Purpose

   Inducibly expresses SRRM2-mCherry in mammalian cells with the Tet-on system

   Depositor

   Wesley Sundquist, Katie Ullman

   Article

   Williams et al Elife. 2021 Aug 4;10. pii: 63743. doi: 1

   Insert

   SRRM2 (SRRM2 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   TREtight

   Available Since

   Sept. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE

   Plasmid

   #60906

   Purpose

   The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP and a NLS for imaging

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   scFv-GCN4
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. hSyn-DIO-somBiPOLES-mCerulean (AAV9)

    Viral Prep

    #154951-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from hSyn-DIO-somBiPOLES-mCerulean (#154951). In addition to the viral particles, you will also receive purified hSyn-DIO-somBiPOLES-mCerulean plasmid DNA. Synapsin-driven, Cre-dependent expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Simon Wiegert

    Article

    Vierock et al Nat Commun. 2021 Jul 26;12(1):4527. doi:

    Promoter

    Syn

    Tags

    mCerulean (Cre-dependent)

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

11. pYTK072

    Plasmid

    #65179

    Purpose

    Encodes ConRE as a Type 5 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    ConRE
    

    Expression

    Bacterial

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV h c-MYC IRES GFP

    Plasmid

    #18119

    Depositor

    John Cleveland

    Insert

    c-MYC (MYC Human)
    

    Use

    Retroviral

    Tags

    GFP

    Mutation

    The CUG which is the start site for Myc I is pres…

    Available Since

    June 9, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti-RfxCas13d-Blast-BFP

    Plasmid

    #196726

    Purpose

    Sortable and selectable RfxCas13d.

    Depositor

    Bernhard Schmierer

    Insert

    RfxCas13d-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    SV40 NLS

    Promoter

    EF1a

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-hEAAT3

    Plasmid

    #32815

    Depositor

    Susan Amara

    Insert

    hEAAT3 (SLC1A1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-PE6d

    Plasmid

    #207854

    Purpose

    Mammalian expression of PE6d prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6d
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    M-MLVRTDRNAseHrt(T128N, V223Y, D200C)

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFX-mCherry-EEA1

    Plasmid

    #174452

    Purpose

    Express mCherry with early endosome-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mCherry with early endosome-targeting sequence
    

    Tags

    early endosomal antigen 1 (EEA1)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3/mStayGold(c4)=UtrCH

    Plasmid

    #212020

    Purpose

    For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    UtrCH
    

    Tags

    mStayGold(c4)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-CAG-jGCaMP8f-WPRE

    Plasmid

    #179254

    Purpose

    AAV-mediated expression of GCaMP8f under the CAG promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8f
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti TurboRFP BlastR

    Plasmid

    #102343

    Purpose

    Lentiviral expression of cytoplasmic TurboRFP with Blasticidin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Parker et al eNeuro. 2018 Sep 27;5(5). pii: eN-MNT-01

    Insert

    TurboRFP
    

    Use

    Lentiviral

    Tags

    N/A

    Promoter

    CMV immediate early

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pmRFP-N1 human cofilin WT

    Plasmid

    #50856

    Purpose

    expresses human cofilin fused to mRFP in mammalian cells

    Depositor

    James Bamburg

    Article

    Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

    Insert

    cofilin 1 (CFL1 Human)
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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