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Showing: 2761 - 2780 of 152518 results

  6. pEAT8-137 M1V

    Plasmid
    #173807
    Purpose
    Expression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cells
    Depositor
    Anne Gershenson
    Article
    Krishnan et al Methods Mol Biol. 2017;1639:195-209. doi
    Insert
    SERPiNA1 (SERPINA1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E1M Otherwise this plasmid encodes the most commo…
    Promoter
    T7
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    173807-plasmid-map-sequence-id-341159

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  7. pDAC565

    Plasmid
    #195242
    Purpose
    Mammalian expression of Csm-GFP complex (RNase mut) from separate promoters (Used for RNA imaging)
    Depositor
    Jennifer Doudna
    Article
    Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103
    Insert
    FLAG-NLS-Csm1; FLAG-NLS-Csm2, FLAG-NLS-Csm3(RNase mut)-GFP; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195242-plasmid-map-sequence-id-382950

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  14. pYPQ230-RRV

    Plasmid
    #188543
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editing
    Depositor
    Yiping Qi
    Article
    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:
    Insert
    LbCas12a-RRV
    Use
    CRISPR; Gateway compatible lbcas12a-rrv entry clo…
    Tags
    NLS
    Expression
    Plant
    Mutation
    G146R, D156R, R182V
    Promoter
    Available since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    188543-plasmid-map-sequence-id-369473

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  15. R777-E111 Hs.IRS2

    Plasmid
    #70395
    Purpose
    Gateway ORF clone of codon optimized human IRS2 [NM_003749] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    IRS2 (IRS2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70395-plasmid-map-sequence-id-125844

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  16. MR015-H11-L2-TALEN

    Plasmid
    #51554
    Purpose
    Expresses the H11-L2 TALEN in mammalian cells. This TALEN was coexpressed with H11-R2 (plasmid #51555) to cleave the H11 locus.
    Depositor
    Michele Calos
    Article
    Zhu et al Nucleic Acids Res. 2013 Dec 4.
    Insert
    H11-L2 TALEN
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51554-plasmid-map-sequence-id-78782

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  17. MR015-H11-R2-TALEN

    Plasmid
    #51555
    Purpose
    Expresses the H11-R2 TALEN in mammalian cells. To cleave the H11 locus, this TALEN was coexpressed with H11-L2 (plasmid #51554).
    Depositor
    Michele Calos
    Article
    Zhu et al Nucleic Acids Res. 2013 Dec 4.
    Insert
    H11-R2 TALEN
    Use
    TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    51555-plasmid-map-sequence-id-78783

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  18. TFORF0706

    Plasmid
    #143432
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NCOR1 (NCOR1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143432-plasmid-map-sequence-id-397919

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  20. pACEBac1-CoRESTfl

    Plasmid
    #109156
    Purpose
    Encodes human full-length CoREST with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system
    Depositor
    Monika Golas
    Article
    Inui et al Protein Sci. 2017 May;26(5):997-1011. do
    Insert
    human full-length CoREST, sequence-optimized for insect cells (RCOR1 Human)
    Use
    Tags
    3xFLAG
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available since
    Jan. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    109156-plasmid-map-sequence-id-232884

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Showing: 2761 - 2780 of 152518 results