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Plasmid#11151PurposeMammalian expression vector for expression of DsRedDepositorHas ServiceCloning Grade DNAInsertDsRed2 from Discosoma sp
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G
Plasmid#167935PurposeInducible CRISPRi expressionDepositorInsertKRAB-dCas9-DHFR
UseLentiviralTagsExpressionMutationPromotertetONAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX458-Ef1a-dCas9-VPR-H2B-mCherry (CRISPRa)
Plasmid#175572PurposeInactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activationDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Cas9-gRNA-GFP
Plasmid#124770Purpose3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backboneDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamKIIa-jGCaMP8s-WPRE (AAV1)
Viral Prep#176752-AAV1PurposeReady-to-use AAV1 particles produced from AAV-CamKIIa-jGCaMP8s-WPRE (#176752). In addition to the viral particles, you will also receive purified AAV-CamKIIa-jGCaMP8s-WPRE plasmid DNA. CamKIIa-driven expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterCamKIIalphaTagsNoneAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
hSyn-DIO-somBiPOLES-mCerulean (AAV9)
Viral Prep#154951-AAV9PurposeReady-to-use AAV9 particles produced from hSyn-DIO-somBiPOLES-mCerulean (#154951). In addition to the viral particles, you will also receive purified hSyn-DIO-somBiPOLES-mCerulean plasmid DNA. Synapsin-driven, Cre-dependent expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmCerulean (Cre-dependent)Available sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV1)
Viral Prep#162381-AAV1PurposeReady-to-use AAV1 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (#162381). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8m (faster, more sensitive). These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
P4 cosmid
Plasmid#196335PurposeP4 cosmid with CRISPR-Cas9 systemDepositorInsertgenes from phage P4, CRISPR-Cas9 system
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (AAV5)
Viral Prep#100833-AAV5PurposeReady-to-use AAV5 particles produced from pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 (#100833). In addition to the viral particles, you will also receive purified pAAV.Syn.Flex.GCaMP6f.WPRE.SV40 plasmid DNA. Syn-driven, Cre-dependent GCaMP6f calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)
Plasmid#208942PurposeThe CE1 construct, expressed from CMV or T7 promoters.DepositorInsertPCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); EcKlenow(-exo;D355A/E357A)PromoterCMV and T7Available sinceNov. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-VSP4-E228Q
Plasmid#80351PurposeExpression of GFP-tagged, dominant negative VPS4 mutantDepositorInsertVPS4-E228Q (VPS4A Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
Su9-EGFP
Plasmid#23214DepositorInsertSubunit 9 of the F0-ATPase
UseTagsEGFPExpressionMammalianMutationcontains AA 1-69 of subunit 9PromoterAvailable sinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-hM3D(Gq)-mCherry (AAV5)
Viral Prep#50478-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-GFAP-hM3D(Gq)-mCherry (#50478). In addition to the viral particles, you will also receive purified pAAV-GFAP-hM3D(Gq)-mCherry plasmid DNA. GFAP-driven hM4D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterGFAPTagsmCherryAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
NGFR
Plasmid#27489DepositorTypeEmpty backboneUseRetroviralTagsECMV, IRES, and NGFRExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-FLAG-GFP-Tmem192
Plasmid#134630Purposelysosomal markerDepositorInsertTMEM192 (TMEM192 Human)
UseLentiviralTagsFLAG and GFPExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
CaMKII-somBiPOLES-mCerulean (AAV9)
Viral Prep#154948-AAV9PurposeReady-to-use AAV9 particles produced from CaMKII-somBiPOLES-mCerulean (#154948). In addition to the viral particles, you will also receive purified CaMKII-somBiPOLES-mCerulean plasmid DNA. CamKII-driven expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIIa(0.4)TagsmCeruleanAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICH47742::p35S-ePEmax3::tEURb7
Plasmid#227692PurposeGolden Gate level 1, position 2, ePEmax3 expression cassette.DepositorInsertePEmax3 being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
UseCRISPRTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVB3011
Plasmid#113389PurposeGalactose-inducible L-BC gag: cure killerDepositorInsertL-BC Gag
UseTagsExpressionYeastMutationPlease see depositor commentsPromoterAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCW-Cas9-2A-EGFP
Plasmid#167928PurposeDoxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.DepositorInsertT2A-EGFP
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotertTRE, hPGKAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only