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Showing: 261 - 280 of 6309 results

  1. pAAV_EF1a_DIO_jGCaMP8f_nano_ribo_WPRE

    Plasmid
    #205283
    Purpose
    GCaMP8f fusion to ribosome nanobody to restrict expression to soma (similar to Chen et al. 2020)
    Depositor
    Matthias Prigge
    Insert
    GCaMP8f
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    205283-plasmid-map-sequence-id-412570

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  4. pENN.AAV.TBG.PI.ffLuciferase.RBG

    Plasmid
    #105538
    Purpose
    AAV expression of firefly luciferase from TBG promoter
    Depositor
    James M. Wilson
    Insert
    Luciferase
    Use
    AAV and Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TBG
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105538-plasmid-map-sequence-id-202429

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  5. pAAV.synP.DIO.EGFP.WPRE.hGH

    Plasmid
    #100043
    Purpose
    An AAV vector that expresses EGFP in a Cre-dependent manner (DIO, double floxed inverted open reading frame).
    Depositor
    Ian Wickersham
    Has Service
    AAV9
    Insert
    EGFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    synapsin-1
    Available since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    100043-plasmid-map-sequence-id-193613

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  6. pENN.AAV.GFAP.iGluSnFr.WPRE.SV40

    Plasmid
    #98930
    Purpose
    AAV expression of glutamate sensor from GFAP promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:
    Has Service
    AAV1, AAV5, and AAV9
    Insert
    iGluSnFr
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    GFAP
    Available since
    Jan. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    98930-plasmid-map-sequence-id-189881

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  9. pENN.AAV.hSyn.TurboRFP.WPRE.RBG

    Plasmid
    #105552
    Purpose
    AAV expression of TurboRFP from hSyn promoter
    Depositor
    James M. Wilson
    Has Service
    AAV1
    Insert
    TurboRFP
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105552-plasmid-map-sequence-id-202449

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  10. AAVS1_puro_CAG_HexaPro

    Plasmid
    #164077
    Purpose
    Targeted integration of SARS-CoV-2 spike HexaPro variant into the AAVS1 genomic safe harbor locus
    Depositor
    Yannick Doyon
    Article
    Rivest et al protocols.io 2021
    Insert
    SARS-CoV-2 S HexaPro (S Severe acute respiratory syndrome coronavirus 2)
    Use
    CRISPR
    Tags
    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CAG
    Available since
    Jan. 4, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    164077-plasmid-map-sequence-id-320680

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  11. pAAV.CAG.Flex.GCaMP6m.WPRE.SV40

    Plasmid
    #100839
    Purpose
    AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter
    Depositor
    GENIE Project, Douglas Kim
    Article
    Chen et al Nature. 2013 Jul 18;499(7458):295-300. d
    Has Service
    AAV1, AAV5, and AAV9
    Insert
    GCaMP6m
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    GCaMP3-T302L R303P M378G K379S D380Y T381R S383T …
    Promoter
    CAG
    Available since
    Nov. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    100839-plasmid-map-sequence-id-189733

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  17. pAAV_hSyn_GCaMP6m_sWPRE_spA_mDLX_nls_mRuby3_ssWPRE_spA

    Plasmid
    #205281
    Purpose
    CaMP used to measuring calcium changes in neurons, while nls-mRuby is employed to specifically identify cortical GABAergic neurons. This dual labeling approach allows for the simultaneous observation of neuronal activity using GCaMP and the identification of a specific subset of neurons using nls-mRuby, focusing specifically on cortical GABAergic neurons.
    Depositor
    Matthias Prigge
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    205281-plasmid-map-sequence-id-412571

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  19. pEH_AAV_TERT_prom_C7C_1.8kb

    Plasmid
    #183079
    Purpose
    rAAV transfer plasmid with ITRs flanking: (1) TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.
    Depositor
    Aviv Regev
    Article
    Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.
    Insert
    TERT wildtype promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)
    Use
    AAV; Homologous recombination donor template
    Tags
    Expression
    Mutation
    Wildtype promoter, Exon 1 C7C (C21>T21) silent…
    Promoter
    None for primary insert (Though partial TERT prom…
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    183079-plasmid-map-sequence-id-363414

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Showing: 261 - 280 of 6309 results