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Showing: 2721 - 2740 of 2891 results

  2. pCali2

    Plasmid
    #86119
    Purpose
    Multiplex temperature-gated expression of mWasabi and mCherry reporters
    Depositor
    Mikhail Shapiro
    Article
    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi
    Inserts
    TlpA36
    mWasabi
    TcI
    mCherry
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    A67T and D341A, P60L, D135V, K187R, K202I, L208Q
    Promoter
    pR/pL, pR/pL, pLacI, pTlpA, and pTlpA, pLacI
    Availability
    Academic Institutions and Nonprofits only
    86119-plasmid-map-sequence-id-173197

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  6. EcNGRfE

    Bacterial Strain
    #48935
    Purpose
    Base line strain is heat shock induce-able lambda red recombination system for MAGE (Multiplex Recombination Genome Engineering)
    Depositor
    George Church
    Bacterial Resistance
    Spectinomycin
    Availability
    Academic Institutions and Nonprofits only

  11. pSG4K5

    Plasmid
    #74492
    Purpose
    For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23119
    Availability
    Academic Institutions and Nonprofits only
    74492-plasmid-map-sequence-id-140493

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  14. EcNGRfC

    Bacterial Strain
    #48936
    Purpose
    Base line strain is heat shock induce-able lambda red recombination system for MAGE (Multiplex Recombination Genome Engineering)
    Depositor
    George Church
    Bacterial Resistance
    Spectinomycin
    Availability
    Academic Institutions and Nonprofits only

  15. EcNGRfEfC

    Bacterial Strain
    #48937
    Purpose
    Base line strain is heat shock induce-able lambda red recombination system for MAGE (Multiplex Recombination Genome Engineering)
    Depositor
    George Church
    Bacterial Resistance
    Spectinomycin
    Availability
    Academic Institutions and Nonprofits only

  20. pcDNA3-DrFLINC-AKAR-Kras

    Plasmid
    #182334
    Purpose
    Genetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.
    Depositor
    Jin Zhang
    Article
    Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951
    Insert
    DrFLINC-AKAR
    Use
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Dronpa contains H193T mutation.
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    182334-plasmid-map-sequence-id-363289

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Showing: 2721 - 2740 of 2891 results