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Showing: 1 - 20 of 2851 results

  2. pCJH026_proD_Cas12c-multiplexing_CAM

    Plasmid
    #183073
    Purpose
    Plasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repression
    Depositor
    Jennifer Doudna
    Article
    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4
    Insert
    Expressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    183073-plasmid-map-sequence-id-358157

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  3. pVD1 Multiplexing Edit En-1 (GB2242)

    Plasmid
    #160564
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the position [5_(n-1)] of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Insert
    Multiplexing Edit (En-1)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160564-plasmid-map-sequence-id-319675

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  6. pVD1 Multiplexing Dicot Edit E1_NTERM (GB2245)

    Plasmid
    #160567
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for position [D1_n] of a monocistronic tRNA-gRNA
    Depositor
    Diego Orzaez
    Article
    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d
    Insert
    Multiplexing Dicot Edit E1_NTERM (Multiplexing Dicot Edit)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    160567-plasmid-map-sequence-id-319681

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  7. Multiplex-CRISPR-miR-200FAM-T2A-GFP

    Plasmid
    #179856
    Purpose
    to delete miR200 family genes including miR-141, miR-200c, miR-429 and miR-200b
    Depositor
    Brendan Jenkins
    Article
    Yu et al Oncogene. 2021 Oct 19. pii: 10.1038/s413
    Insert
    hsa-miR-200 family (miR-200b, miR-200a, miR-429, miR-141 and miR-200c ) (MIR141, MIR200B, MIR429, MIR200C, MIR200A Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    179856-plasmid-map-sequence-id-357409

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  8. pECNUS4_MP

    Plasmid
    #228358
    Purpose
    ABE8e-SpCas9-NG Multiplex Acceptor clone for insertion of up to six gRNA cassettes via Golden Gate with BpiI and T4 ligase, blue-white screening
    Depositor
    Eunyoung Chae
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    RPS5A
    Available since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    228358-plasmid-map-sequence-id-455680

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  16. pEE491

    Plasmid
    #149292
    Purpose
    T-DNA encoding TRV2 with tRNA multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2
    Depositor
    Daniel Voytas
    Article
    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41
    Insert
    p35S:TRV2_tRNA_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    July 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    149292-plasmid-map-sequence-id-305826

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  18. pEE531

    Plasmid
    #149290
    Purpose
    T-DNA encoding TRV2 with spacer multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2
    Depositor
    Daniel Voytas
    Article
    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41
    Insert
    p35S:TRV2_Spacer_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    July 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    149290-plasmid-map-sequence-id-305881

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  20. pEE495

    Plasmid
    #149291
    Purpose
    T-DNA encoding TRV2 with direct repeat multiplexed truncated FT augmented gRNAs targeting NbAGsgRNA1, NbPDS3, NbAGsgRNA2
    Depositor
    Daniel Voytas
    Article
    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41
    Insert
    p35S:TRV2_Direct_NbAGsgRNA1_NbPDS3sgRNA_NbAGsgRNA2_TrunFT:tNos
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    35S
    Available since
    July 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    149291-plasmid-map-sequence-id-305898

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Showing: 1 - 20 of 2851 results