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Plasmid#82502PurposeVector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. G418 resistance is encoded by the Meo gene.DepositorInserteGFP-IRESMeo
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTORGm43GW
Plasmid#112902PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin.DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-IcaR-e
Plasmid#51176DepositorInsertIcaR
UseSynthetic BiologyTags6x-hisExpressionBacterialMutationPromoterT7Available sinceAug. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTORGm43GWR
Plasmid#112905PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of tdTomato.DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTORGm43GWY
Plasmid#112906PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mCitrine.DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTORGm43GWC
Plasmid#112903PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mTFP1.DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTORGm43GWF
Plasmid#112904PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mWasabi.DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRL-ubi63E
Plasmid#74280PurposeRenilla control plasmid driven by the ubiquitin 63E promoterDepositorInsertRenilla luciferase
UseTagsExpressionInsectMutationPromoterubiquitin 63E promoterAvailable sinceApril 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAMS366-4xCDRE-GFP
Plasmid#138657Purposestable GFP reporter to monitor calcineurin activity via calcineurin-dependent response element (CDRE) in S. cerevisiaeDepositorInsert4xCDRE-CYC1(PM)-yeGFP
UseYeast reporter plasmidTagsExpressionMutationPromoterCYC1Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRF-QacR
Plasmid#49369DepositorInsertQacR repressor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGGF001
Plasmid#48842PurposeProvides a BASTA resistance cassette as GreenGate module.DepositorInsertpMAS::BastaR::tMAS
UseGolden gate compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ClfB_N2N3-HIS-ybbr
Plasmid#101717PurposeClfB N2 and N3 domains with C-terminal ybbr tag for covalent immobilizationDepositorInsertClfB_N2N3
UseTagsH3V 3C, HIS, and ybbrExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRF-IcaR
Plasmid#49362DepositorInsertIcaR repressor
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
SaMscL (CDelta26)
Plasmid#79027PurposeStaphylococcus aureus MscL with C Terminal Truncation of 26 amino acidsDepositorInsertMscL
UseTags6x Histidine TagExpressionMutationStop codon placed before 26 amino acids at C Term…PromoterT7 PromoterAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pFBD-ESCRT 0
Plasmid#21499DepositorInsertpFastBac Dual-His/MBP/Hrs-GST/STAM1 (STAM Human, Mouse)
UseTagsGST and His-MBPExpressionInsectMutationnonePromoterAvailable sinceSept. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SdrG_N2N3-HIS-ybbr
Plasmid#101238PurposeSdrG N2 and N3 domains with C-terminal ybbr tag for covalent immobilizationDepositorInsertSdrG_N2N3
UseTagsH3V 3C, HIS, and ybbrExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAMS366-4xCDRE-GFP-PEST
Plasmid#138658Purposedestabilized GFP reporter to monitor calcineurin activity via calcineurin-dependent response element (CDRE) in S. cerevisiaeDepositorInsert4xCDRE-CYC1(PM)-yEGFP3-PEST
UseYeast reporter plasmidTagsExpressionMutationPromoterCYC1Available sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTH1-SaSrtA-Q
Plasmid#64980PurposeExpresses the E108Q mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
UseTagsHis6 tag, Mxe GyrA intein, and chitin-binding pro…ExpressionBacterialMutationdeleted amino acids 1-59, E108QPromoterAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-hChR2(C128S)-EYFP-WPRE
Plasmid#20294PurposeLentiviral expression of ChR2 (C128S) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsEYFPExpressionMammalianMutationchanged Cysteine 128 to SerinePromoterAvailable sinceMay 13, 2009AvailabilityAcademic Institutions and Nonprofits only