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Plasmid#176225PurposeLambdaRed-expressing plasmid carrying the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phageDepositorInsertLambda Red operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaB and PLAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMDC123SB-AtMIR390a-B/c
Plasmid#51775PurposePlant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursorDepositorInsertAtMIR390a-B/c
UsePlant expressionTagsExpressionMutationA. thaliana MIR390a precursor sequence including …PromoterAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFK210B-AtMIR390a-B/c
Plasmid#51777PurposePlant expression vector (35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursorDepositorInsertAtMIR390a-B/c
UsePlant expressionTagsExpressionMutationA. thaliana MIR390a precursor sequence including …PromoterAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-AtMIR390a-B/c
Plasmid#51778PurposeGateway-compatible entry vector for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursorDepositorInsertAtMIR390a-B/c
UseGateway entry vectorTagsExpressionMutationA. thaliana MIR390a precursor sequence including …PromoterAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-AtTAS1c-B/c
Plasmid#51773PurposePlant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursorDepositorInsertAtTAS1c-B/c
UsePlant expressionTagsExpressionMutationA. thaliana TAS1c precursor sequence including a …PromoterAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTU2-A-RFP (p15A origin)
Plasmid#74090PurposepTU2-A backbone, with RFP negative selection and p15A (low-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 4 TU's with RFP and p15A origin
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterR0010 Const. Promoter controlling RFPAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNatA (pACYCduet-naa10-naa15)
Plasmid#72928PurposeAllows expression of a modified version of the fission yeast NatA complex - chloramphenicol markerDepositorUseTagsExpressionBacterialMutationCodon optomised for E.coli expressionPromoterT7Available sinceFeb. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMDC123SB-AtTAS1c-B/c
Plasmid#51772PurposePlant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursorDepositorInsertAtTAS1c-B/c
UsePlant expressionTagsExpressionMutationA. thaliana TAS1c precursor sequence including a …PromoterAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTU2S-b (p15A origin)
Plasmid#74089PurposeSecondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 3 TU's with secondary module and p15A origin
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-AtTAS1c-B/c
Plasmid#51774PurposeGateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursorDepositorInsertAtTAS1c-B/c
UseGateway-compatible entry cloneTagsExpressionMutationA. thaliana TAS1c precursor sequence including a …PromoterAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTU2-A-RFP (colE1 origin)
Plasmid#74093PurposepTU2-A backbone, with RFP negative selection and colE1 (medium-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 4 TU's with RFP and colE1 origin of replication
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterR0010 Const. Promoter controlling RFPAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKA397
Plasmid#80380Purposemini-Tn7 delivery plasmid with metE under the cpcG2Δ59 promoter.DepositorInsertscat
metE
UseSynthetic Biology; Mini-tn7 delivery vectorTagsExpressionBacterialMutationPromotercat promoter and cpcG2Δ59Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTU2S-b (colE1 origin)
Plasmid#74092PurposeSecondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replicationDepositorInsertGolden Gate destination vector - Level 2, 3 TU's with secondary module and colE1 origin
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterNoneAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
psbA2-Ptrc-T7-g10-PHLS (e)
Plasmid#52310PurposeReplaces the psbA2 gene in Synechocystis with the Ptrc promoter-T7-g10-PHLS geneDepositorInsertsbeta-phellandrene synthase
Chloramphenicol resistance
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtrc-T7Available sinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCK015
Plasmid#105347PurposeBacterial translocation fo proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionTagsExpressionMutationBsaI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTC3cIfm
Plasmid#193778PurposeExpresses frame-shifted cI under the control of PLTetO-1 promoter, p15A origin of replication, Chloramphenicol selectionDepositorInsertFrame-shifted CI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPLTetO-1Available sinceApril 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCK013
Plasmid#105345PurposeBacterial translocation fo proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionTagsExpressionMutationBsaI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK016
Plasmid#105348PurposeBacterial translocation for proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionTagsExpressionMutationBsaI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK014
Plasmid#105346PurposeBacterial translocation fo proteins into plant cellsDepositorInsertsecretion signal, ccdB cassette
UseBacterial secretionTagsExpressionMutationBsaI restriction sites removedPromoterAvailable sinceMay 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETDest
Plasmid#124245PurposeAllows Gateway cloning into the bacterial 6xHIS tagged protein expression vector pET26DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only