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PurposePlant expression vector (2x35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursor
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 51776 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMDC32
- Backbone size w/o insert (bp) 10133
- Total vector size (bp) 12044
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Modifications to backboneA BsaI site of the backbone was mutated to block the site.
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Vector typePlant Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Kanamycin, 25 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Growth instructionsE. coli DB3.1 (or other strain resistant to ccdB) at 37C
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAtMIR390a-B/c
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SpeciesA. thaliana (mustard weed)
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Insert Size (bp)1911
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MutationA. thaliana MIR390a precursor sequence including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites. The BsaI site in the ccdB gene was mutated to block the site.
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13-Rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMDC32B-AtMIR390a-B/c was a gift from James Carrington (Addgene plasmid # 51776 ; http://n2t.net/addgene:51776 ; RRID:Addgene_51776) -
For your References section:
New Generation of Artificial MicroRNA and Synthetic Trans-Acting Small Interfering RNA Vectors for Efficient Gene Silencing in Arabidopsis. Carbonell A, Takeda A, Fahlgren N, Johnson SC, Cuperus JT, Carrington JC. Plant Physiol. 2014 Mar 19. 10.1104/pp.113.234989 PubMed 24647477