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Plasmid#73208PurposeExpresses dL5(E52D)-mCer3 fusion protein in mitochondria of mammalian cells. (MBIC5, dL5**, FAP)DepositorInsertCOXIV-COX8-dL5-2XG4S-mCer3 (COX8A Synthetic, Human)
UseTagsThe FAP and mCerulean3 are fused with 2 copies of…ExpressionMammalianMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterCMVAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL
Plasmid#73209PurposeExpresses myc-dL5(E52D)-mCer3 fusion protein in endoplasmic reticulum of mammalian cells with an Igk-leader. (MBIC5, dL5**, FAP)DepositorInsertkappa-myc-dL5-2XG4S-mCer3-KDEL (MYC Synthetic, Human)
UseTagsThe FAP and mCerulean3 are fused with 2 copies of…ExpressionMammalianMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterCMVAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21-10XHis-GST-HRV-dL5-Her1
Plasmid#73215PurposeBacterial expression vector for dL5(E52D)-Z1907 affibody fusion protein (FA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)DepositorInsertEGFR (EGFR Synthetic, Human, Schistosoma japonicum)
UseTags10XHis-GST-HRV and The dL5 FAP and Her1 affibody …ExpressionBacterialMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterT7Available sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL
Plasmid#73210PurposeExpresses myc-dL5(E52D)-mCer3 fusion protein in peroxisomes of mammalian cells with a C-terminal PTS (SKL). (MBIC5, dL5**, FAP)DepositorInsertKozATG-myc-dL5-2XG4S-mCer3-SKL (MYC Synthetic, Human)
UseTagsThe FAP and mCerulean3 are fused with 2 copies of…ExpressionMammalianMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterCMVAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21-10XHis-GST-HRV-Her1-dL5-Her1
Plasmid#73217PurposeBacterial expression vector for Z1907-dL5(E52D)-Z1907 affibody fusion protein (AFA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP, AffiFAP)DepositorInsert10XHis-GST-HRV-Her1-dL5-Her1 (EGFR Synthetic, Human, Schistosoma japonicum)
UseTags10XHis-GST-HRV and The Her1 Affibody and dL5 FAP …ExpressionBacterialMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterT7Available sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21-10XHis-GST-HRV-Her1-dL5
Plasmid#73216PurposeBacterial expression vector for Z1907-dL5(E52D) affibody fusion protein (AF) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)DepositorInsert10XHis-GST-HRV-Her1-dL5 (EGFR Synthetic, Human, Schistosoma japonicum)
UseTags10XHis-GST-HRV and The Her1 Affibody and dL5 FAP …ExpressionBacterialMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterT7Available sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-IgKappa-myc-dL5- 2XG4S-GPI
Plasmid#153308PurposeExpresses GPI-anchored dL5** in mammalian cells. (FAP-GPI)DepositorInsertIgKappa-myc-dL5-2XG4S-GPI
UseTagsmyc-dL5ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-dL5**-mCER-TRF1
Plasmid#168176PurposeExpresses FAP (dL5**) fused to mCerulean and the telomere binding protein TRF1DepositorInsertTRF1 (TERF1 Human)
UseLentiviralTagsFAP (dL5**) and mCeruleanExpressionMammalianMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-10XHis-GST-HRV-dL5
Plasmid#73214PurposeBacterial expression vector for dL5(E52D) protein with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)DepositorInsertEGFR (EGFR Human, Schistosoma japonicum)
UseTags10XHis-GST-HRVExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe-kappa-HA-dL5-myc-ADRB2
Plasmid#101253PurposeExpresses HA-dL5(E52D)-cMyc N-terminal fusion of beta-2 adrenergic receptor in mammalian cells, MMLV retroviral plasmid (MBIC5, dL5**, FAP, ADRB2)DepositorInsertkappa-HA-dL5-myc-ADRB2 (ADRB2 Budding Yeast, Human)
UseRetroviralTagsFAP-dL5 and HA epitopeExpressionMammalianMutationPromoterMMLV LTRAvailable sinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a- hPTP1e-PDZ2
Plasmid#20377DepositorInserthPTP1e PDZ2 (PTPN13 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
EGFP-PH-dL5**
Plasmid#96947PurposeExpresses EGFP-PLC pleckstrin homology domain linked to FAP for CALI validationDepositorInsertPleckstrin homology domain
UseTagsEGFP and dL5ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
PH-dL5**
Plasmid#96946PurposePLC pleckstrin homology domain linked to FAP for CALI validationDepositorInsertPleckstrin homology domain
UseTagsdL5 and mCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ MBIC5-mCer3
Plasmid#74116PurposeFor generating mRNA for zebrafish injection of cytoplasmic fluorogen-activating protein (FAP)DepositorInsertMBIC5-mCer3
UseTransgenic constructTagsmCerulean 3ExpressionMutationPromoterMyl7 (Cmlc2)Available sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD-DiB2
Plasmid#168801PurposeFluorogen activating protein (FAP) DiB2DepositorInsertFluorogen activating protein DiB2
UseTagsHis-tagExpressionBacterialMutationPromoteraraBADAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-DiB1
Plasmid#168800PurposeFluorogen activating protein (FAP) DiB1DepositorInsertFluorogen activating protein DiB1
UseTagsHis-tagExpressionBacterialMutationPromoteraraBADAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-DiB3
Plasmid#168802PurposeFluorogen activating protein (FAP) DiB3DepositorInsertFluorogen activating protein DiB3
UseTagsHis-tagExpressionBacterialMutationPromoteraraBADAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-TagBFP-DiB-RM
Plasmid#168782PurposeActin filament reporter, LifeAct, fused with blue fluorescent protein TagBFP and fluorogen activating protein (FAP) DiB-RMDepositorInsertLifeAct-TagBFP-DiB-RM
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSYC-201
Plasmid#178963PurposeHuman GFAP fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombinationDepositorInsertsUseTol2 mediated recombinationTags3xFLAGExpressionMutationPromoterzebrafish gfap promoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSYC-204
Plasmid#178964PurposeHuman GFAP (R79C) mutant fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombinationDepositorInsertsUseTol2 mediated recombinationTags3xFLAGExpressionMutationR79CPromoterZebrafish gfap promoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only