pET21-10XHis-GST-HRV-Her1-dL5
(Plasmid
#73216)
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PurposeBacterial expression vector for Z1907-dL5(E52D) affibody fusion protein (AF) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)
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Depositing Lab
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Sequence Information
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Sequences (3) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 73216 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET21a
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5387
- Total vector size (bp) 7046
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Modifications to backbonenone
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsFor expression use a strain that contains T7 RNA polymerase and trxB/gor double mutant such as Rosetta-gami(DE3)
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Copy numberLow Copy
Gene/Insert
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Gene/Insert name10XHis-GST-HRV-Her1-dL5
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Alt nameHL
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Alt nameAF
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SpeciesH. sapiens (human), Synthetic; Schistosoma japonicum
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Insert Size (bp)1662
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MutationThe dL5** FAP is E50D, L89S, also known as E52D/L91S and NP138
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Entrez GeneEGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)
- Promoter T7
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Tags
/ Fusion Proteins
- 10XHis-GST-HRV (N terminal on insert)
- The Her1 Affibody and dL5 FAP are fused with 8 amino acids between
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI and HindIII for Affibody-FAP (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 and GSTSeqF
- 3′ sequencing primer T7term (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit the Molecular Biosensor and Imaging Center website for current protocols and information about dyes to be used in combination with the proteins expressed from this plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET21-10XHis-GST-HRV-Her1-dL5 was a gift from Marcel Bruchez (Addgene plasmid # 73216 ; http://n2t.net/addgene:73216 ; RRID:Addgene_73216) -
For your References section:
Fluorogen activating protein-affibody probes: modular, no-wash measurement of epidermal growth factor receptors. Wang Y, Telmer CA, Schmidt BF, Franke JD, Ort S, Arndt-Jovin DJ, Bruchez MP. Bioconjug Chem. 2015 Jan 21;26(1):137-44. doi: 10.1021/bc500525b. Epub 2014 Dec 19. 10.1021/bc500525b PubMed 25490520