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Plasmid#146072PurposeInsect Expression of DmTral-LSm-R21EE23KS13AI15ADepositorInsertDmTral-LSm-R21EE23KS13AI15A (tral Fly)
UseTagsExpressionInsectMutationone silent mutation A198C compared to the sequenc…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmTral-DFDF-KFKF_C
Plasmid#146038PurposeInsect Expression of DmTral-DFDF-KFKFDepositorInsertDmTral-DFDF-KFKF (tral Fly)
UseTagsExpressionInsectMutationthree silent mutations A198C, A387G, A1437G and 4…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-D30KT35A_C
Plasmid#146042PurposeInsect Expression of DmTral-D30KT35ADepositorInsertDmTral-D30KT35A (tral Fly)
UseTagsExpressionInsectMutationtwo silent mutations A198C, A387G and a mutation …PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-del46-60_C
Plasmid#146043PurposeInsect Expression of DmTral-del46-60DepositorInsertDmTral-del46-60 (tral Fly)
UseTagsExpressionInsectMutationtwo silent mutations A198C, A387G and a mutation …PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-F44A_C
Plasmid#146044PurposeInsect Expression of DmTral-F44ADepositorInsertDmTral-F44A (tral Fly)
UseTagsExpressionInsectMutationtwo silent mutations A198C, A387G and a mutation …PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-K73AD74A_C
Plasmid#146045PurposeInsect Expression of DmTral-K73AD74ADepositorInsertDmTral-K73AD74A (tral Fly)
UseTagsExpressionInsectMutationtwo silent mutations A198C, A387G and a mutation …PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmTral-R42E_B
Plasmid#145988PurposeInsect Expression of DmTral-R42EDepositorInsertDmTral-R42E (tral Fly)
UseTagsExpressionInsectMutationtwo silent mutations A198C, A387G and a mutation …PromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-INTS3-C
Plasmid#128642PurposeExpress INTS3 C-termini (514-1042 aa) in E. coli with N-terminal and C-terminal His tagDepositorInsertINTS3 C-termini (INTS3 Human)
UseTagsHis tag on N and C-terminalExpressionBacterialMutationINTS3 C-termini (514–1042 aa)PromoterT7Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIVM_26
Plasmid#204501PurposeExpression of EloB (1-104) and EloC (17-112) for production of VCB complexes in conjunction with plasmid pIVM_02 (VHL 54-213)DepositorUseTagsExpressionBacterialMutation0PromoterAmpRAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
MS2-P65-HSF1_GFP
Plasmid#61423PurposeExpresses the MS2-P65-HSF1 activator helper complex with a 2A GFP. 3rd generation lentiviral vector.DepositorInsertMS2(N55K)-P65-HSF1_2A_GFP (HSF1 Synthetic, Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationN55K in MS2PromoterEF1AAvailable sinceDec. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1(nsp8-nsp7)(nsp12)
Plasmid#165451PurposeExpresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia ColiDepositorInsertsnsp12
nsp8
nsp7
UseTags14Histidine-TEVExpressionBacterialMutationPromoterT7Available sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO {ChETA-mRuby2}on-W3SL
Plasmid#111389PurposeAAV vector with hSynapsin promoter, Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)DepositorInsertsChETA-mRuby2
W3SL
UseAAVTagsmRuby2ExpressionMammalianMutationW3SL is built from a shortened WPRE, an upstream …PromoterhSynapsinAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
p2R3a-VenusYFP-3AT
Plasmid#71269PurposeEntry clone containing Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertVenusYFP
UseGatewayTags4xGly linker and T3A pea Pisum sativum ribulose-1…ExpressionMutationPromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
RP1B FUS 1-163 QQ4xSS #1
Plasmid#127194PurposeProtein expression for affinity purificationDepositorInsertFUS 1-163 QQ4xSS #1 (FUS Human)
UseTagshexahistidineExpressionBacterialMutationQQ4xSS #1: Q27S, Q28S, Q93S, Q94S, Q132S, Q133S, …PromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.2EmGFP-cDAXX-0SB
Plasmid#169728PurposeMammalian expression construct of GFP tagged cDAXX-0SBDepositorInsertcDAXX-0SB (DAXX Human)
UseTagsGFPExpressionMammalianMutationComprises residues 495-741 and has the major SB m…PromoterCMVAvailable sinceJune 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NDUFV2-mVenus
Plasmid#185653PurposeNDUFV2 Complex I sub-unit with C-terminus mVenus tagDepositorInsertNDUFV2 (NDUFV2 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NDUFS6-mVenus
Plasmid#185806PurposeNDUFS6 Complex I sub-unit with C-terminus mVenus tagDepositorInsertNDUFS6 (NDUFS6 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN-T2dMFot
Plasmid#44558DepositorInsertsPTETREG promoter
rtetR-M2::FFF
UseSynthetic Biology; Expression regulator/reporterTagsExpressionYeastMutationCompared to rtTA, rtTA-M2 has S12G, E19G, A56P, D…PromoterPTETREGAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NDUFA7-mVenus
Plasmid#185647PurposeNDUFA7 Complex I sub-unit with C-terminus mVenus tagDepositorInsertNDUFA7 (NDUFA7 Human)
UseTagsmVenusExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
RP1B FUS 1-163 QQ4xSS #2
Plasmid#127195PurposeProtein expression for affinity purificationDepositorInsertFUS 1-163 QQ4xSS #2 (FUS Human)
UseTagshexahistidineExpressionBacterialMutationQQ4xSS #2:Q8S, Q9S, Q60S, Q62S, Q102, Q103, Q139S…PromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only