pET28a-INTS3-C
(Plasmid #128642)

• Purpose: Express INTS3 C-termini (514-1042 aa) in E. coli with N-terminal and C-terminal His tag
• Depositing Lab: Yuliang Wu
• Publication: Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi: 10.1042/BCJ20170351.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 6889
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: INTS3 C-termini
• Alt name: Integrator Complex Subunit 3 (INTS3)
• Alt name: SOSS-A
• Alt name: C1orf60
• Species: H. sapiens (human)
• Insert Size (bp): 1587
• Mutation: INTS3 C-termini (514–1042 aa)
• GenBank ID: NM_023015.5
• Entrez Gene: INTS3 (a.k.a. C1orf193, C1orf60, INT3, SOSS-A, SOSSA)
• Promoter: T7
• Tag / Fusion Protein:
  • His tag on N and C-terminal

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nhe I (not destroyed)
• 3′ cloning site: Xho I (not destroyed)
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry