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Plasmid#114045PurposeExpression of exogenous LAP tagged MPS1 Kinase-deadDepositorInsertMPS1-KD (TTK Human)
UseTagsExpressionMammalianMutationD664A kinase deadPromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
yHS1-M7A
Plasmid#159151PurposeCytosolic labile heme reporterDepositorInsertyHS1-M7A
UseTagsExpressionYeastMutationMutated Met 7 of the cyt b562 module of yHS1 to A…PromoterGPDAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
GPR78-Tango
Plasmid#66374PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGPR78 (GPR78 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
shPBRM1 #122-Tet-pLKO-puro
Plasmid#107401PurposeLentiviral expression of shRNA targeting PBRMDepositorInsertshRNA targeting PBRM1 (PBRM1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO shGATA6 puro
Plasmid#72615PurposeExpresses an inducible short hairpin targeting human GATA6 sequenceDepositorInsertshort hairpin GATA binding protein 6
UseLentiviralTagsNoneExpressionMammalianMutationPromoterTRE TightAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNAIIIB HA JNK
Plasmid#61630Purposemammalian expression of JNKDepositorInsertJNK1 beta1 isoform (MAPK8 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
sgPBRM1-1- LentiCRISPRv2
Plasmid#107403PurposeLentiviral expression of Cas9 and gRNA targeting PBRM1DepositorInsertgRNA targeting PBRM1 (PBRM1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MfPylRS (human-opti)
Plasmid#164081PurposeTo express a Methanosarcina flavescens derived PylRS in mammalian cells for genetic code expansionDepositorInsertMfPylRS(human-opti)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgPBRM1-2- LentiCRISPRv2
Plasmid#107404PurposeLentiviral expression of Cas9 and gRNA targeting PBRM1DepositorInsertgRNA targeting PBRM1 (PBRM1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5 CASTOR2-Flag Cterm
Plasmid#84490Purposeoverexpression of CASTOR2 with Flag tagDepositorInsertCASTOR2 (GATSL2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-Ef1a-DIO-hBFP-2A-RVG-WPRE
Plasmid#120268PurposePermits retrograde transsynaptic spread by driving Cre-dependent co-expression of the RbG plus a nuclear reporterDepositorInsertEF1α-DIO-H2B-tagBFP-Flagx3-T2Am-cB19G
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-OSF-BECN1
Plasmid#99328PurposeComponent for mammalian expression of recombinant PI3KC3-C1 complexDepositorInsertBeclin 1 (BECN1 Human)
UseTagsStrep-Strep-FlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T-Actin-7
Plasmid#58001PurposeLocalization: Actin, Excitation: 555, Emission: 584DepositorInsertActin (ACTB Human)
UseTagsmTagRFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
GFP-Golgin-84-TEV #709#243
Plasmid#42108DepositorInsertGolgin-84 (GOLGA5 Human)
UseTagsGFPExpressionMammalianMutationinserted ENLYFQG b/w 300 and 301PromoterCMVAvailable sinceFeb. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBabe(puro) mouse Egr3
Plasmid#108102PurposeRetroviral vector for expression of mouse Egr3DepositorInsertEgr3 (Egr3 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterSV40Available sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLPC-mCh-ACTB-P2A-EGFP
Plasmid#165158PurposeExpresses an ACTB gene fragment with P2A, flanked by mCherry and EGFP, in Mammalian cellsDepositorInsertmCherry-ACTB-P2A-EGFP (ACTB Human)
UseRetroviralTagsEGFP and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceApril 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1 3xFlag-JNK1a1
Plasmid#47574PurposeBacterial expression plasmid for GST-3xFlag-tagged JNK1a1DepositorInsertJNK1a1 (MAPK8 Human)
UseTags3xFlag and GSTExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKnockinDonor-humanCTCF-miniAID-mClover3
Plasmid#136883Purposeknockin donor vector of miniAID-mClover3 to C-terminus of endogenous human CTCFDepositorInsertCTCF-knockin-homology arm-miniAID-mClover3
UseTagsminiAID-mClover3ExpressionMammalianMutationPromoterno promoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mGold-Actin-C-18
Plasmid#158009PurposeVisualization of actin in mammalian cells using mGoldDepositorInsertActin (ACTB Human)
UseTagsmGoldExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only