pCMV-mGold-Actin-C-18
(Plasmid
#158009)
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PurposeVisualization of actin in mammalian cells using mGold
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 158009 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV
- Total vector size (bp) 5778
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameActin
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SpeciesH. sapiens (human)
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GenBank IDNM_001101.3
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Entrez GeneACTB (a.k.a. BKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8)
- Promoter CMV
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Tag
/ Fusion Protein
- mGold
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Existing fluorescent protein in plasmid Adggene# 56395 was replaced with mGold.
"C-18" of the name of the plasmid indicates the position of the tag (N- or C-terminal with respect to the fluorescent protein) and the number of residues in the linker between the two proteins. Please note that the mVenus annotation in the plasmid map is incorrect, as this plasmid encodes mGold.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-mGold-Actin-C-18 was a gift from Francois St-Pierre (Addgene plasmid # 158009 ; http://n2t.net/addgene:158009 ; RRID:Addgene_158009) -
For your References section:
Versatile phenotype-activated cell sorting. Lee J, Liu Z, Suzuki PH, Ahrens JF, Lai S, Lu X, Guan S, St-Pierre F. Sci Adv. 2020 Oct 23;6(43). pii: 6/43/eabb7438. doi: 10.1126/sciadv.abb7438. Print 2020 Oct. 10.1126/sciadv.abb7438 PubMed 33097540