Showing: 2201 - 2220 of 26766 results
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Plasmid#185087PurposeSwi6L-GFP with nuclear export signal mutated to alaninesDepositorInsertSWI6
UseTagsGFPExpressionYeastMutationSwi6 nuclear export signal L to A mutationsPromoterAvailabilityAcademic Institutions and Nonprofits only -
KBB418
Plasmid#185088PurposeSwi6L-GFP with nuclear export signal mutated to alaninesDepositorInsertSWI6
UseTagsGFPExpressionYeastMutationSwi6 nuclear export signal L to A mutationsPromoterAvailabilityAcademic Institutions and Nonprofits only -
pET21b-LaG9(S151C)
Plasmid#172490PurposeEncodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).DepositorInsertLaG9(S151C)
UseTags6xHis, PPX cleavage site, and pelB leader sequenc…ExpressionBacterialMutationS151CPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pQE80l BCB
Plasmid#196472PurposeProtein expression of SpyCatcher-ELP-CarHC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcherDepositorInsertBCB
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
U6-sgRNA(MS2)_EF1a-MS2-P65-HSF1
Plasmid#92120PurposeExpression plasmid for both MS2-P65-HSF1 activator helper complex and sgRNA with two MS2 loops at tetraloop and stemloop 2 contains BsaI sites for insertion of spacer sequences.DepositorInsertMS2-P65-HSF1 (HSF1 Human, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterEF1aAvailabilityAcademic Institutions and Nonprofits only -
pSH-Csy4-T2A-SpRFN
Plasmid#85754PurposeExpresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).DepositorInsertCsy4-T2A-FokI-dCas9
UseCRISPRTagsCsy4 and FokI fusion via 25 amino acid (GGGGS)5 l…ExpressionMammalianMutationD10A, H840APromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pSH-Csy4-T2A-SaRFN
Plasmid#85755PurposeExpresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).DepositorInsertCsy4-T2A-FokI-SadCas9
UseCRISPRTags3xHA tag, Csy4, and FokI fusion via 36 amino acid…ExpressionMammalianMutationD10A, N580APromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
FLT1-KDR
Plasmid#86034Purposechimera of human VEGFR1 extracellular domain with the TMD fused to the intracellular domain of VEGFR2DepositorUseTagsHA and t-GFPExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-Cas9/CD4-TK2
Plasmid#104397PurposeThe designed sgRNA cloned into this plasmid directs the specific DNA cleavage exerted by Cas9 nuclease in a region of exon 5 of the human TK2 gene. The plasmid also includes the Cas9 nuclease and CD4.DepositorInsertTK2 (TK2 Human)
UseTagsExpressionMammalianMutationPromoterU6AvailabilityAcademic Institutions and Nonprofits only -
pHOT-P2Y2R
Plasmid#159108Purposeentiviral vector encoding P2Y purinoceptor 2 (P2Y2R), a high affinity G-protein coupled receptor that mobilizes Ca2+ from intracellular stores upon binding extracellular ATP.48,49 By using the GibsonDepositorInsertP2Y2R
UseLentiviralTagsExpressionMutationPromoterUBCAvailabilityAcademic Institutions and Nonprofits only -
NLS-4LNDDE488Q-pCDNA3.1(+)
Plasmid#172314PurposeExpresses ADAR2 Deaminase Domain (DD) hyperactive mutant E488Q fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS)DepositorInsertLambda N peptide - human ADAR2 Deaminase Domain E488Q
UseTags6xHis, Flag, and Nuclear Localization Signal of S…ExpressionMammalianMutationchanged the Glutamic acid 488 in the Deaminase Do…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pENTR4 CDS A. thaliana PARP2
Plasmid#175810PurposepENTR4 plasmid with CDS of Arabidopsis thaliana POLY(ADP-RIBOSE) POLYMERASE 2 (PARP2, AT4G02390.1) without stop codon for C-terminal epitope tagsDepositorInsertPARP2 (PARP2 Mustard Weed)
UsePentr plasmid for gateway cloningTagsnoneExpressionMutationPromoternoneAvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-LZ_VNp-LZ-mNeongreen-hGH
Plasmid#182425PurposeBacterial expression of Vesicle Nucleating peptide-Leucine Zipper and Vesicle Nucleating peptide-Leucine Zipper-mNeongreen-human growth hormone fusion proteinsDepositorInsertsUseTagsVesicle Nucleating peptide (VNp) and Vesicle Nucl…ExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5L18-CtermCan
Plasmid#51438PurposeExpresses TALEN targeting left CCR5A site (L18) with canonical C-terminal domainDepositorInsertTALEN CCR5 Left (L18) Can C-term
UseTALENTags3xFLAG and Fok1 EL variantExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5R18-CtermCan
Plasmid#51439PurposeExpresses TALEN targeting right CCR5A site (R18) with canonical C-terminal domainDepositorInsertTALEN CCR5 Right (R18) Can C-term
UseTALENTags3xFLAG and Fok1 KK variantExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5L18-CtermQ3
Plasmid#51440PurposeExpresses TALEN targeting left CCR5A site (L18) with Q3 C-terminal domainDepositorInsertTALEN CCR5 Left (L18) Q3 C-term
UseTALENTags3xFLAG and Fok1 EL variantExpressionMammalianMutationK788Q, R792Q and R801QPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5R18-CtermQ3
Plasmid#51441PurposeExpresses TALEN targeting right CCR5A site (R18) with Q3 C-terminal domainDepositorInsertTALEN CCR5 Right (R18) Q3 C-term
UseTALENTags3xFLAG and Fok1 KK variantExpressionMammalianMutationK788Q, R792Q and R801QPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5L18-NtermN1
Plasmid#51444PurposeExpresses TALEN targeting left CCR5A site (L18) with N1 N-terminal domainDepositorInsertTALEN CCR5 Left (L18) N1 N-term
UseTALENTags3xFLAG and Fok1 EL variantExpressionMammalianMutationK150QPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5R18-NtermN1
Plasmid#51445PurposeExpresses TALEN targeting right CCR5A site (R18) with N1 N-terminal domainDepositorInsertTALEN CCR5 Right (R18) N1 N-term
UseTALENTags3xFLAG and Fok1 KK variantExpressionMammalianMutationK150QPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
TALEN-CCR5L18-NtermN2
Plasmid#51446PurposeExpresses TALEN targeting left CCR5A site (L18) with N2 N-terminal domainDepositorInsertTALEN CCR5 Left (L18) N1 N-term
UseTALENTags3xFLAG and Fok1 EL variantExpressionMammalianMutationK150Q and K153QPromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 2201 - 2220 of 26766 results