SuExp His-Myc-mPLD3
(Plasmid #173853)

• Purpose: Protein expression plasmid for recombinant His-Myc tag mouse PLD3
• Depositing Lab: David Nemazee
• Publication: Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi: 10.1038/s41467-021-26150-w.

Backbone

• Vector backbone: SuExp
• Backbone manufacturer: Nemazee lab (Deli)
• Backbone size w/o insert (bp): 2000
• Total vector size (bp): 3600
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Bleocin (Zeocin), 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PLD3
• Species: M. musculus (mouse)
• Insert Size (bp): 1500
• Mutation: intracellular and transmembrane domain truncated, with only extracellular domain
• GenBank ID: 18807
• Entrez Gene: Pld3 (a.k.a. Sam-9)
• Promoter: CMV
• Tag / Fusion Protein:
  • 6xHis, Myc, Factor X cleavable (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40 pA-R

Resource Information

• Supplemental Documents:
  • SuExp-His-Myc-mPLD3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry