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Showing: 2181 - 2200 of 3228 results

  4. pX458-Dual-Guide-Donor-Cas9-H2B-mCherry

    Plasmid
    #175570
    Purpose
    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.
    Depositor
    Joseph Brzezinski
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    XbaI site added upstream of U6 promotor. G to C …
    Promoter
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    175570-plasmid-map-sequence-id-344992

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  8. pJB06

    Plasmid
    #190480
    Purpose
    Base Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis system
    Depositor
    Joseph Sorg
    Article
    Brehm et al Microbiol Resour Announc. 2022 Dec 15;11
    Inserts
    Cas9
    XylR
    Use
    E. coli / b. subtilis - c. difficile shuttle vect…
    Tags
    Expression
    Mutation
    Promoter
    xylR-driven promoter
    Available since
    Oct. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    190480-plasmid-map-sequence-id-374623

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  9. pJB07

    Plasmid
    #190481
    Purpose
    Targeting plasmid for 2-plasmid C. difficile mutagenesis system
    Depositor
    Joseph Sorg
    Article
    Brehm et al Microbiol Resour Announc. 2022 Dec 15;11
    Inserts
    Upstream & Downstream pyrE deletion region
    gRNA
    Use
    E. coli / b. subtilis - c. difficile shuttle vect…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190481-plasmid-map-sequence-id-372027

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  12. pKM197

    Plasmid
    #132665
    Purpose
    pyrE-targeted CRISPR-Cas9 control plasmid. Cas9 expression is controlled by the xylR promoter and results in improved conjugation efficiency. Induction with xylose results in a pyrE mutant.
    Depositor
    Joseph Sorg
    Article
    Bhattacharjee et al mSphere. 2020 Mar 4;5(2):e00941-19. doi:
    Insert
    Upstream & Downstream pyrE deletion region
    Use
    E. coli - c. difficile shuttle vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    132665-plasmid-map-sequence-id-259679

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  17. MK1283

    Plasmid
    #71428
    Purpose
    This E. coli DH10B strain harbors a shuttle vector plasmid that expresses the Mixed Feedback Loop version of the UBER system with a T7RNAP translation rate of 1535 and a TetR translation rate of 30709
    Depositor
    Howard Salis
    Article
    Kushwaha et al Nat Commun. 2015 Jul 17;6:7832. doi: 10.
    Insert
    Mixed Feedback Loop version of the UBER system
    Use
    Tags
    Expression
    Mutation
    T7 RBS: AACCGAGCCCAATATAGGACCTAGGGTGCCAAAAAA and …
    Promoter
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71428-plasmid-map-sequence-id-440608

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Showing: 2181 - 2200 of 3228 results