Skip to main content
Addgene
Showing: 1981 - 2000 of 14448 results

  1. pAAV-EF1α1.1-FLEX-rc [Jaws-KGC-GFP-ER2]

    Plasmid
    #108274
    Purpose
    AAV-mediated expression of Jaws-KGC-GFP-ER2 under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.
    Depositor
    Edward Boyden
    Article
    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi
    Insert
    Jaws-KGC-GFP-ER2
    Use
    AAV
    Tags
    ER2, GFP, and KGC
    Expression
    Mammalian
    Mutation
    K200R W214F
    Promoter
    EF1α promoter (1.1kb short version)
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    108274-plasmid-map-sequence-id-262430

    Enlarge

  2. pEXSISERS_mGreenLantern_FRT-PuroR-HSV-TK

    Plasmid
    #177868
    Purpose
    Cloning Backbone for mGreenLantern-based EXSISERS containing FRT-sites flanked PuroR-HSV-TK cassette; clone homology arms via BbsI (or BpiI).
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2021 Jun;23(6):652-663. d
    Insert
    mGreenLantern-based EXSISERS
    Use
    CRISPR, Synthetic Biology, and Unspecified
    Tags
    OLLAS
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    177868-plasmid-map-sequence-id-358838

    Enlarge

  3. pLXSN p110 CUX1

    Plasmid
    #90471
    Purpose
    Retroviral vector expressing human p110 CUX1 (amino acids 747-1505) with a Myc and HA tag at the N- and C-terminue, respectively
    Depositor
    Alain Nepveu
    Article
    Kedinger et al J Biol Chem. 2009 Oct 2;284(40):27701-11
    Insert
    CUX1 (amino acids 747-1505) (CUX1 Human)
    Use
    Retroviral
    Tags
    HA and Myc
    Expression
    Mammalian
    Mutation
    Promoter
    Moloney murine leukemia virus long terminal repeat
    Available since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    90471-plasmid-map-sequence-id-205963

    Enlarge

  5. pGL4.23-hs737.3

    Plasmid
    #222475
    Purpose
    Luciferase vector containing the hs737 enhancer sequence (sequence containing variant 3). Variant 3 is chr10:128569437-G-A where the information is chromosome:positionInB38-RefAllele-AltAllele.
    Depositor
    Tychele Turner
    Article
    Padhi et al Hum Genomics. 2021 Jul 13;15(1):44. doi:
    Insert
    hs737 enhancer sequence (sequence containing variant 3) (LOC110120928 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Variant 3 is chr10:128569437-G-A where the inform…
    Promoter
    Available since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    222475-plasmid-map-sequence-id-442952

    Enlarge

  6. pGL4.23-hs737.2

    Plasmid
    #222474
    Purpose
    Luciferase vector containing the hs737 enhancer sequence (sequence containing variant 2). Variant 2 is chr10:128569418-T-C where the information is chromosome:positionInB38-RefAllele-AltAllele.
    Depositor
    Tychele Turner
    Article
    Padhi et al Hum Genomics. 2021 Jul 13;15(1):44. doi:
    Insert
    hs737 enhancer sequence (sequence containing variant 2) (LOC110120928 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Variant 2 is chr10:128569418-T-C where the inform…
    Promoter
    Available since
    Aug. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    222474-plasmid-map-sequence-id-442951

    Enlarge

  7. pRP[CRISPR]-EGFP/Puro-hCas9-U6>(long left)-U6>(long right)

    Plasmid
    #216871
    Purpose
    This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23
    Depositor
    Ori Bar-Nur
    Article
    Lenardic et al J Clin Invest. 2024 May 7:e166998. doi:
    Insert
    Cas9, gRNA Long.L, gRNA Long.R (Dmd Mouse)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    mdx mutation in mouse dystrophin
    Promoter
    Available since
    June 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    216871-plasmid-map-sequence-id-435357

    Enlarge

  9. pUt-mNF-BACH1

    Plasmid
    #199219
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with GFP-BACH1 Negative-Feedback circuit (mNF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    hTetR::P2A::EGFP::BACH1 (BACH1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV D2ir promoter
    Available since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    199219-plasmid-map-sequence-id-396467

    Enlarge

  10. pUt-mPF-BACH1 Cas9-Resistant_AmpR

    Plasmid
    #199221
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Positive-Feedback circuit (mPF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    rtTA-Advanced::P2A::EGFP::BACH1 (BACH1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    silient mutation at nucleic acid 177 C changed to…
    Promoter
    tight TRE promoter
    Available since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199221-plasmid-map-sequence-id-396541

    Enlarge

  11. pUt-mNF-BACH1 Cas9-Resistant

    Plasmid
    #199218
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Negative-Feedback circuit (mNF-BACH1 Cas9- resistant). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    hTetR::P2A::EGFP::BACH1 (BACH1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    silient mutation at nucleic acid 177 C changed to…
    Promoter
    CMV D2ir promoter
    Available since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199218-plasmid-map-sequence-id-396470

    Enlarge

  12. pCR2.1-myco

    Plasmid
    #36870
    Depositor
    Mario Chin
    Insert
    myco
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    36870-plasmid-map-sequence-id-46090

    Enlarge

  13. pENTR4-SNAPf (w878-1)

    Plasmid
    #29652
    Depositor
    Eric Campeau
    Type
    Empty backbone
    Use
    Entry vector
    Tags
    SNAPf
    Expression
    Mutation
    Promoter
    Available since
    May 11, 2011
    Availability
    Academic Institutions and Nonprofits only
    29652-plasmid-map-sequence-id-220455

    Enlarge

  14. pENTR4-CLIPf (w877-2)

    Plasmid
    #29650
    Depositor
    Eric Campeau
    Type
    Empty backbone
    Use
    Entry vector
    Tags
    CLIPf
    Expression
    Mutation
    Promoter
    Available since
    July 6, 2011
    Availability
    Academic Institutions and Nonprofits only
    29650-plasmid-map-sequence-id-13765

    Enlarge

  15. pCR4-myco

    Plasmid
    #36872
    Depositor
    Mario Chin
    Insert
    myco
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 20, 2012
    Availability
    Academic Institutions and Nonprofits only
    36872-plasmid-map-sequence-id-46092

    Enlarge

  17. pgLAP1

    Plasmid
    #19702
    Depositor
    Peter Jackson
    Type
    Empty backbone
    Use
    Tags
    EGFP, S peptide, and TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2009
    Availability
    Academic Institutions and Nonprofits only
    19702-plasmid-map-sequence-id-9330

    Enlarge

  18. pgLAP2

    Plasmid
    #19703
    Depositor
    Peter Jackson
    Type
    Empty backbone
    Use
    Tags
    Flag, S peptide, and TEV
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 19, 2009
    Availability
    Academic Institutions and Nonprofits only
    19703-plasmid-map-sequence-id-9331

    Enlarge

  19. pCEFL

    Plasmid
    #38121
    Depositor
    JS Gutkind
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1
    Available since
    Sept. 7, 2012
    Availability
    Academic Institutions and Nonprofits only
    38121-plasmid-map-sequence-id-48259

    Enlarge

  20. pENTR4-HaloTag (w876-1)

    Plasmid
    #29644
    Depositor
    Eric Campeau
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Entry vector
    Tags
    HaloTag
    Expression
    Mutation
    Promoter
    Available since
    May 11, 2011
    Availability
    Academic Institutions and Nonprofits only
    29644-plasmid-map-sequence-id-220459

    Enlarge

Showing: 1981 - 2000 of 14448 results