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Plasmid#134345PurposeMammalian acoustic reporter gene 1 cassette 3 on the piggyBac trasposon plasmid.DepositorInsertmARG 1 cassete 3
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
V5-BS2-SENP2 (Nuclear Pore)
Plasmid#220345PurposeMammalian expression vector BS2 in nuclear poreDepositorInsertBS2
UseTagsSENP2 (nuclear pore localized protein) and V5 tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMT1002
Plasmid#8621DepositorInsertbarnase, barstar
UseTagsas pMT416ExpressionBacterialMutationCI157 lambda repressor gene has two HindIII sites…PromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCS-lpp0.2-K64A
Plasmid#201789Purposemutant of PadR; transcriptional regulator can respond to p-coumaric acidDepositorInsertPadR (padR Bacillus subtilis)
UseTagsExpressionBacterialMutationK64APromoterAvailable sinceJuly 12, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS-lpp0.2-H38A
Plasmid#201788Purposemutant of PadR; transcriptional regulator can respond to p-coumaric acidDepositorInsertPadR (padR Bacillus subtilis)
UseTagsExpressionBacterialMutationH38APromoterAvailable sinceJuly 12, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUAST-NachBac-EGFP
Plasmid#40282DepositorInsertNaChBac-EGFP
UseDrosophilaTagsEGFPExpressionMutationPromoterAvailable sinceOct. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMZ2007
Plasmid#204399PurposeA 3OC6-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsquorum sensing regulator LuxR
GFP
UseSynthetic BiologyTagsExpressionMutationPromoterPftsH and Synthetic promoter PLux0002Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWalium-ChtVis-Tomato
Plasmid#67756PurposeExpresses a fluorescent chitin reporter under Gal4 control in Drosophila.DepositorInsertdyl - chitin binding domain - tdTomato - dyl
UseTagstd-tomatoExpressionInsectMutationPromoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCG-D-SpyTag-His6-E
Plasmid#87375PurposeAn ORF part-containing vector harbouring the SpyTag sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF assemblyDepositorInsertSpyTag
UseSynthetic BiologyTagsHis6 tagExpressionMutationPromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCG-B-mSpyCatcher-C
Plasmid#87374PurposeAn ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assemblyDepositorInsertmSpyCatcher
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHT-trxA.ads
Plasmid#47383PurposeExpresses ads with trxA tag under Pgrac promoter (inducible by IPTG) in bacteriaDepositorInsertads
UseTags6xHis and trxAExpressionBacterialMutationPromoterPgracAvailable sinceOct. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBsaHFm
Plasmid#85103PurposeHeme-FMN domain of P450 BM3 variant (A74G)DepositorInsertHeme-FMN domain of P450 BM3
UseCloning vectorTagsExpressionMutationA74GPromoterAvailable sinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL0_115 [pBC1 ORI]
Plasmid#199015PurposeLevel 0 partDepositorInsertpBC1 ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBsaFa
Plasmid#85104PurposeFAD domain of P450 BM3 variant (C773S/C810S)DepositorInsertFADdomain of P450 BM3
UseCloning vectorTagsExpressionMutationC773S/C810SPromoterAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLIC.B4.BSU37660
Plasmid#11535DepositorInsertphosphotransacetylase
UseTagsHisTag-MBP, TEV cleavableExpressionBacterialMutationPromoterAvailable sinceApril 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMZ3012
Plasmid#204400PurposeA C4-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.DepositorInsertsUseSynthetic BiologyTagsExpressionMutationPromoterPftsH and Synthetic promoter PRhl0L0L12Available sinceDec. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDR111_Bgal no NLS
Plasmid#188397PurposeExpresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.DepositorInsertlacZ
UseTagsPhoD secretion peptideExpressionBacterialMutationPromoterPhyper spankAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pP3-GFP
Plasmid#53436PurposeControls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genomeDepositorInsertP3 promoter
UseSynthetic BiologyTagsnoneExpressionBacterialMutationnonePromoterP3Available sinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRBBm59
Plasmid#48115PurposeShuttle vector E. coli/B.meg.DepositorInsertpromoter sacB - gfp
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDR111_mannose_Bgal no NLS
Plasmid#188399PurposeExpresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.DepositorInsertlacZ
UseTagsPhoD secretion peptideExpressionBacterialMutationPromoterD-mannoseAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only